@ARTICLE{Antanaitis_R._Impact_2022, author={Antanaitis, R. and Anskienė, L. and Televičius, M. and Malašauskienė, D. and Šertvytytė, G. and Stoškus, R. and Meškinytė, E. and Rous, J. and Helm, B.}, volume={vol. 25}, number={No 4}, journal={Polish Journal of Veterinary Sciences}, pages={535-546}, howpublished={online}, year={2022}, publisher={Polish Academy of Sciences Committee of Veterinary Sciences}, publisher={University of Warmia and Mazury in Olsztyn}, abstract={The aim of this study was to investigate the impact of feed supplements with alfa-amylase and beta-glucanase (Optipartum C+ 200) on ingestive-related behaviour biomarkers registered with real-time sensors: rumination behaviours and reticulorumen parameters (pH and temperature). Cows (n=20) in the treatment group (TG) were fed with Optipartum C+ 200 (Enzymes feed supplement: Alfa-Amylase 57 Units; Beta-Glucanase 107 Units) from 21 days before calving until 30 days after calving with a feeding rate of 200 g/cow/day. Cows (n=22) in the control group (CG) were fed a feed ration without feed supplement. Measurements started from 6 days before calving and continued until 21 days after calving. The following indicators were registered: with the RumiWatch System: Rumination time; Eating time; Drinking time; Rumination chews; Eating chews; Drinking gulps; Bolus; Chews per minute; Chews per bolus. With the SmaXtec system: the temperature, pH of the contents of the cows’ reticulorumens, and cows’ walking activity. According to our results, feed supplementation with alfa-amylase and beta-glucanase (Optipartum C+ 200) in the TG group resulted in increases in the following parameters: 9% rumination time and eating time, 19% drinking time, 11% rumination chews, 16% eating chews, 13% number of boluses per rumination, 5% chews per minute and 16% chews per bolus. The rumination time showed a strong, positive relation with rumination chews and bolus indicators in both groups (TG and CG) (p<0.001); while the rumination time in both groups of cows showed an opposite direction and was negatively related to eating time and eating chews (p<0.05). We found a 1.28 % lower reticulorumen pH and a 0.64 % lower reticulorumen temperature in cows fed with the supplement compared with cows in the control group. Cows in TG were 8.80% more active than those in the CG group. For improvement of ingestive-related behaviour we suggest adding a feed supplement with alfa-amylase and beta-glucanase (Optipartum C+ 200).}, type={Article}, title={Impact of feed supplement with alfa-amylase and beta-glucanase on ingestive-related biomarkers registered with real-time sensors}, URL={http://journals.pan.pl/Content/125605/PDF-MASTER/5%20_%20Antanaitis.pdf}, doi={10.24425/pjvs.2022.142041}, keywords={alfa-amylase, beta-glucanase, reticulorumen, rumination}, }