@ARTICLE{Jaworska_Małgorzata_M._Enzymatic_2024,
 author={Jaworska, Małgorzata M. and Biniaś, Dorota and Dąbkowska-Susfał, Katarzyna and Górak, Andrzej},
 volume={vol. 45},
 number={No 1},
 journal={Chemical and Process Engineering: New Frontiers},
 pages={e53},
 howpublished={online},
 year={2024},
 publisher={Polish Academy of Sciences Committee of Chemical and Process Engineering},
 abstract={Despite its unique properties (biocompatibility and nontoxicity), chitin itself has limited application. Chitin is completely insoluble in most organic or inorganic solvents what can be beneficial when chitin is investigated as a support for chromatography or enzyme immobilization. These applications require the particles to have an extensive outer surface with a large number of reactive ligands. The increase in specific surface area of chitin particles can be performed by dissolution in ionic liquid and precipitation with water. To increase the number of reactive ligands (amine groups), deacetylation of the surface of chitin particles is necessary. The deacetylation process can be carried out by an enzymatic process with the enzyme, chitin deacetylase. In our investigation, 21 ionic liquids were used for chitin particle structure modification followed by enzymatic deacetylation. Results proved positive effect of modifications with ionic liquid on enzymatic deacetylation of the chitin surface with chitin deacetylase. For 12 samples the deacetylation gave an increase in number of active ligands in comparison to natural chitin. The best results were observed for [Bmim][Br], [Emim][Cl] and [MPpip][Ac]. That could be correlated with an increase in outer surface area by increasing porosity of particles or by structural changes in chitin particles.},
 type={Article},
 title={Enzymatic deacetylation of chitin treated with ionic liquids},
 URL={http://journals.pan.pl/Content/129353/PDF/e53_CPE-00122-2023-02-Accepted%20Article.pdf},
 doi={10.24425/cpe.2023.147412},
 keywords={chitin, chitin deacetylase, ionic liquids, enzymatic deacetylation},
}