@ARTICLE{Jańczak_Dawid_PCR-based_2023,
 author={Jańczak, Dawid and Górecki, Piotr and Maj, Aleksandra Kornelia},
 volume={vol. 26},
 number={No 4},
 journal={Polish Journal of Veterinary Sciences},
 pages={629-634},
 howpublished={online},
 year={2023},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={Dermatophytes from Microsporum, Trichophyton and Epidermophyton genera are divided into geophilic, zoophilic and anthropophilic species which cause skin infection in humans and wide group of animals, mainly mammals. Main species causing dermatophytosis in dogs and cats are Microsporum and Trichophyton. Conventional mycological diagnostic technique includes Saburaud Dextrose Agar (SAD) and others medium cultures, 10% KOH mount and direct microscopy of hairs and scraping. Molecular diagnostic become more frequent in veterinary practice due to shortening of waiting time. In this study we based on two PCR methods. The nested PCR amplified CHS1 gene for dermatophytes detection, and multiplex PCR coding ITS1 and ITS2 fragments for species identification of detected derpatophytes. Most frequently detected species was Microsporum canis, mainly in young cats. Geophilic Microsporum gypseum and anthropophilic Trichophyton rubrum was found primarily in dogs. Molecular methods in dermatophytosis identification are rapid in contrast to routinely, long lasting culture.},
 type={Article},
 title={PCR-based methods in detection and identification of dermatophytes in dogs and cats with suspected dermatophytosis in 2021 in Poland},
 URL={http://journals.pan.pl/Content/129586/PDF/10%20_%20Janczak.pdf},
 doi={10.24425/pjvs.2023.148282},
 keywords={dermatophytes, dogs, cats, Poland, nested PCR, multiplex PCR},
}