TY - JOUR N2 - The aims of this study were to evaluate the prevalence of Coxiella burnetii on both herd and animal level based on ELISA and PCR tests. Antibodies to C. burnetii were detected in 22 out of the 24 bulk tank milk samples (91.6%) tested by ELISA and the IS1111 element of C. burnetii was detected in 10 out of the 24 samples (41.6%) by real-time polymerase chain reaction (PCR). ELISA testing showed individual seropositivity in 67 out of the 165 cows (40.6%) examined in 24 dairy cattle farms in different parts of the Czech Republic. Our study revealed that the prevalence of C. burnetii has increased substantially in the Czech Republic over the past 30 years, and that the causative agent is a potential risk factor for some reproductive problems in dairy farms and a possible risk factor for human infection. L1 - http://journals.pan.pl/Content/123687/PDF-MASTER/5%20_%20Dobos.pdf L2 - http://journals.pan.pl/Content/123687 PY - 2022 IS - No 2 EP - 235 DO - 10.24425/pjvs.2022.141807 KW - Q fever KW - surveillance KW - ELISA KW - real-time PCR KW - bulk tank milk A1 - Dobos, A. A1 - Fodor, I. A1 - Tekin, T. A1 - Đuričić, D. A1 - Samardzija, M. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 25 DA - 2022.07.10 T1 - Presence of Coxiella burnetii in dairy cattle and farms in the Czech Republic SP - 231 UR - http://journals.pan.pl/dlibra/publication/edition/123687 T2 - Polish Journal of Veterinary Sciences ER -