TY - JOUR N2 - Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein. L1 - http://journals.pan.pl/Content/125608/PDF-MASTER/8%20_%20Uslu.pdf L2 - http://journals.pan.pl/Content/125608 PY - 2022 IS - No 4 EP - 569 KW - rOMP19 antigen KW - Brucella melitensis KW - recombinant protein A1 - Uslu, A. A1 - Sanioglu Golen, G. A1 - Agah Tekindal, M. A1 - Sakmanoglu, A. A1 - Sayın, Z. A1 - Denizli, O. A1 - Gok, A. A1 - Erganis, O. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 25 DA - 2023.01.05 T1 - Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19 SP - 561 UR - http://journals.pan.pl/dlibra/publication/edition/125608 T2 - Polish Journal of Veterinary Sciences ER -