TY - JOUR N2 - The aim of the study was to verify the quality of ram semen, frozen in 1982-1983, from the historical collection of the Bank of Biological Material of the National Research Institute of Animal Production. A total of 18 ejaculates from 3 Świniarka type rams were analyzed to assess sperm motility (subjectively), total motility, progressive motility, sperm concentration (CASA), membrane integrity (SYBR-14/PI) and chromatin structure (SCSA). In order to determine sperm fertilizing ability 49 ewes were intracervically inseminated (200×106 sperm per AI) with frozen- thawed semen 12 and 24 hours after detection of estrus. Sperm motility parameters, membrane intact spermatozoa and DFI did not differ among the analyzed rams. Spermatozoa concentration was significantly higher for ram no. 2 than for rams no. 1 and 3. The lambing rates (27.3 to 36.0%) did not differ significantly for individual rams. The ram semen, which had been stored for around 40 years, showed satisfactory quality and fertilizing capacity, allowing for its use in artificial insemination. L1 - http://journals.pan.pl/Content/125617/PDF-MASTER/17%20_%20Gogol%20_%20Short.pdf L2 - http://journals.pan.pl/Content/125617 PY - 2022 IS - No 4 EP - 633 DO - 10.24425/pjvs.2022.143544 KW - ram semen KW - ex situ storage KW - gene banks KW - cryopreservation KW - sperm quality KW - fertility A1 - Gogol, P. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 25 DA - 2023.01.05 T1 - The quality and fertilizing ability of the ram semen cryopreserved around 40 years ago SP - 631 UR - http://journals.pan.pl/dlibra/publication/edition/125617 T2 - Polish Journal of Veterinary Sciences ER -