TY - JOUR N2 - In flowering plants, seeds are produced both sexually (double fertilization is required) and asexually via apomixis (meiotic reduction and egg fertilization are omitted). An apomictic-like pattern of endosperm development in planta is followed by fis mutants of sexual Arabidopsis thaliana. In our experiments in planta, autonomous endosperm (AE) developed in met1 mutants. Furthermore we obtained autonomous endosperm formation in vitro not only in unfertilized ovules of fie mutants but also in wild genotypes (Col-0, MET1/MET1, FIE/FIE) and met1 mutants. AE induction and development occurred in all genotypes on the each of the media used and in every trial. The frequency of AE was relatively high (51.2% ovaries) and genotype-dependent. AE induced in vitro represents a more advanced stage of development than AE induced in fie mutants in planta. This was manifested by a high number of nuclei surrounded by cytoplasm and organized in nuclear cytoplasmic domains (NCDs), nodule formation, division into characteristic regions, and cellularization. The high frequency of AE observed in homozygous met1 (met1/met1) mutants probably is due to accumulation of hypomethylation as an effect of the met1 mutation and the in vitro conditions. AE development was most advanced in FIE/fie mutants. We suggest that changes in the methylation of one or several genes in the DNA of Arabidopsis genotypes caused by in vitro conditions resulted in AE induction and/or further AE development. L1 - http://journals.pan.pl/Content/81808/PDF/15_rojek.pdf L2 - http://journals.pan.pl/Content/81808 PY - 2013 IS - No 2 DO - 10.2478/abcsb-2013-0032 KW - Arabidopsis KW - fie and met1 mutants KW - apomixis KW - autonomous endosperm KW - in vitro culture KW - methylation A1 - Rojek, Joanna A1 - Kuta, Elżbieta A1 - Kapusta, Małgorzata A1 - Ihnatowicz, Anna A1 - Bohdanowicz, Jerzy PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 55 DA - 2013 T1 - THE INFLUENCE OF fie AND met1 MUTATIONS AND IN VITRO CULTURE CONDITIONS ON AUTONOMOUS ENDOSPERM DEVELOPMENT IN UNFERTILIZED OVULES OF ARABIDOPSIS THALIANA UR - http://journals.pan.pl/dlibra/publication/edition/81808 T2 - Acta Biologica Cracoviensia s. Botanica ER -