TY - JOUR N2 - Antibiotics are a group of substances potentially harmful to the environment. They can play a role in bacterial resistance transfer among pathogenic and non-pathogenic bacteria. In this experiment three representatives of medically important chemotherapeutics, confirmed to be present in high concentrations in wastewater treatment plants with HPLC analysis were used: erythromycin, sulfamethoxazole and trimethoprim. Erythromycin concentration in activated sludge was not higher than 20 ng L−1. N-acetylo-sulfamethoxazole concentration was 3349 ± 719 in winter and 2933 ± 429 ng L−1 in summer. Trimethoprim was present in wastewater at concentrations 400 ± 22 and 364 ± 60 ng L−1, respectively in winter and summer. Due to a wide variety of PCR-detectable resistance mechanisms towards these substances, the most common found in literature was chosen. For erythromycin: erm and mef genes, for sulfamethoxazole: sul1, sul2, sul3 genes, in the case of trimethoprim resistance dhfrA1 and dhfr14 were used in this study. The presence of resistance genes were analyzed in pure strains isolated from activated sludge and in the activated sludge sample itself. The research revealed that the value of minimal inhibitory concentration (MIC) did not correspond with the expected presence of more than one resistance mechanisms. Most of the isolates possessed only one of the genes responsible for a particular chemotherapeutic resistance. It was confirmed that it is possible to monitor the presence of resistance genes directly in activated sludge using PCR. Due to the limited isolates number used in the experiment these results should be regarded as preliminary. L1 - http://journals.pan.pl/Content/86102/PDF/10265-Volume41-Issue4_paper_03.pdf L2 - http://journals.pan.pl/Content/86102 PY - 2015 IS - No 4 DO - 10.1515/aep-2015-0035 KW - DGGE KW - PCR KW - sulfamethoxazole KW - trimethoprim and erythromycin resistance KW - WWTP A1 - Ziembińska-Buczyńska, Aleksandra A1 - Felis, Ewa A1 - Folkert, Justyna A1 - Meresta, Anna A1 - Stawicka, Dominika A1 - Gnida, Anna A1 - Surmacz-Górska, Joanna PB - Polish Academy of Sciences VL - vol. 41 DA - 2015[2015.01.01 AD - 2015.12.31 AD] T1 - Detection of antibiotic resistance genes in wastewater treatment plant – molecular and classical approach UR - http://journals.pan.pl/dlibra/publication/edition/86102 T2 - Archives of Environmental Protection ER -