Reduced deformed wing virus of Apis mellifera L. nurses by high fat diets under laboratory conditions

Journal title

Journal of Plant Protection Research




vol. 61


No 1


Alshukri, Baida Mohsen : Plant Protection Department, University of Kufa, Najaf Governorate, Iraq ; Al-Esawy, Mushtaq Talib : Plant Protection Department, University of Kufa, Najaf Governorate, Iraq ; Al-Esawy, Mushtaq Talib : Biosciences Institute, Newcastle University, Newcastle upon Tyne, United Kingdom



deformed wing virus ; fat diets ; honey bee ; nutrition ; protein

Divisions of PAS

Nauki Biologiczne i Rolnicze




Committee of Plant Protection PAS ; Institute of Plant Protection – National Research Institute


1. Alaux C., Dantec C., Parrinello H., Le Conte Y. 2011. Nutrigenomics in honey bees: digital gene expression analysis of pollen's nutritive effects on healthy and varroa-parasitized bees. BMC genomics 12 (1): 496. DOI:
2. Alaux C., Ducloz F., Crauser D., Le Conte Y. 2010. Diet effects on honeybee immunocompetence. Biology Letters: rsbl20090986. DOI: https://doi: 10.1186/1471-2164-12-496.
3. Basualdo M., Barragan S., Vanagas L., Garcia C., Solana H., Rodriguez E., Bedascarrasbure E. 2013. Conversion of high and low pollen protein diets into protein in worker honey bees (Hymenoptera: Apidae). Journal of Economic Entomology 106 (4): 1553–1558. DOI:
4. Benaets K., Van Geystelen A., Cardoen D., De Smet L., de Graaf D. C., Schoofs L., Larmuseau M.H., Brettell L.E., Martin S.J., Wenseleers T. 2017. Covert deformed wing virus infections have long-term deleterious effects on honeybee foraging and survival. Proceedings of the Royal Society B: Biological Sciences 284 (1848), 25 pp. DOI:
5. Branchiccela B., Castelli L., Corona M., Díaz-Cetti S., Invernizzi C., de la Escalera G.M., Mendoza Y., Santos E., Silva C., Zunino P. 2019. Impact of nutritional stress on the honeybee colony health. Scientific Reports 9 (1): 1–11. DOI:
6. Brodschneider R., Crailsheim K. 2010. Nutrition and health in honey bees. Apidologie 41 (3): 278–294. DOI:
7. Crailsheim K. 1991. Interadult feeding of jelly in honeybee (Apis mellifera L.) colonies. Journal of Comparative Physiology B 161 (1): 55–60. DOI:
8. Dainat B., Evans J.D., Chen Y.P., Gauthier L., Neumann P. 2012. Predictive markers of honey bee colony collapse. PLoS one 7 (2): e32151. DOI: https://
9. DeGrandi-Hoffman G., Chen Y., Huang E., Huang M.H. 2010. The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.). Journal of Insect Physiology 56: 1184–1191. DOI:
10. deGroot A. 1953. Protein and amino acid requirements of the honey bee (Apis mellifera L.). Phys Comp Oec 3: 197–285. DOI:
11. Di Pasquale G., Salignon M., Le Conte Y., Belzunces L.P., Decourtye A., Kretzschmar A., Suchail S., Brunet J.-L., Alaux C. 2013. Influence of pollen nutrition on honey bee health: do pollen quality and diversity matter? PloS One 8 (8): e72016. DOI:
12. Di Prisco G., Annoscia D., Margiotta M., Ferrara R., Varricchio P., Zanni V., Caprio E., Nazzi F., Pennacchio F. 2016. A mutualistic symbiosis between a parasitic mite and a pathogenic virus undermines honey bee immunity and health. Proceedings of the National Academy of Sciences 113 (12): 3203–3208. DOI:
13. Forzan M., Felicioli A., Sagona S., Bandecchi P., Mazzei M. 2017. Complete genome sequence of deformed wing virus isolated from Vespa crabro in Italy. Genome Announc 5 (40): e00961–00917. DOI:
14. Goodman W.G., Cusson M. 2012. The juvenile hormones p. 310–365. In: "Insect Endocrinology" (L.I. Gilbert, ed.). San Diego, Academic Press. CA, USA.
15. Goulson D., Nicholls E., Botías C., Rotheray E. L. 2015. Bee declines driven by combined stress from parasites, pesticides, and lack of flowers. Science 347 (6229): 1–16. DOI: 10.1126/science.1255957
16. Highfield A.C., El Nagar A., Mackinder L.C., Noel L.M., Hall M.J., Martin S.J., Schroeder D.C. 2009. Deformed wing virus implicated in overwintering honeybee colony losses. Applied Environmental Microbiology 75 (22): 7212–7220. DOI:
17. Im S.-S., Yousef L., Blaschitz C., Liu J.Z., Edwards R.A., Young S.G., Raffatellu M., Osborne T.F. 2011. Linking lipid metabolism to the innate immune response in macrophages through sterol regulatory element binding protein-1a. Cell Metabolism 13 (5): 540–549. DOI: https://doi.10.1016/j.cmet.2011.04.001
18. Jackman J.A., Cho N.-J. 2020. Supported lipid bilayer formation: beyond vesicle fusion. Langmuir 36 (6): 1387–1400. DOI: 10.1021/acs.langmuir.9b03706
19. Martin S.J., Brettell L.E. 2019. Deformed wing virus in honeybees and other insects. Annual Review of Virology 6: 49–69. DOI:
20. Moore J., Jironkin A., Chandler D., Burroughs N., Evans D.J., Ryabov E.V. 2011. Recombinants between Deformed wing virus and Varroa destructor virus-1 may prevail in Varroa destructor-infested honeybee colonies. Journal of General Virology 92 (1): 156–161. DOI: 10.1099/vir.0.025965-0
21. Ponton F., Wilson K., Cotter S.C., Raubenheimer D., Simpson S.J. 2011. Nutritional immunology: a multi-dimensional approach. PLoS Pathogens 7 (12): e1002223. DOI:
22. Ponton F., Wilson K., Holmes A.J., Cotter S.C., Raubenheimer D., Simpson S.J. 2013. Integrating nutrition and immunology: a new frontier. Journal of Insect Physiology 59 (2): 130–137. DOI:
23. Roulston T.A.H., Cane J.H., Buchmann S.L. 2000. What governs protein content of pollen: pollinator preferences, pollen-pistil interactions, or phylogeny? Ecological Monographs 70 (4): 617–643. DOI:[0617:WGPCOP]2.0.CO;2
24. Smilanich A.M., Mason P.A., Singer M.S. 2014. Ecological immunology mediated by diet in herbivorous insects. Integrative and Comparative Biology 54 (5): 913–921. DOI: https://
25. Staroscik A. 2004. Calculator for determining the number of copies of a template. URI Genomics and Sequencing Center.
26. Tantillo G., Bottaro M., Di Pinto A., Martella V., Di Pinto P., Terio V. 2015. Virus Infections of honeybees Apis mellifera. Italian Journal of Food Safety 4 (3): 5364–5364. DOI:
27. Vaudo A.D., Stabler D., Patch H.M., Tooker J.F., Grozinger C.M., Wright G.A. 2016. Bumble bees regulate their intake of essential protein and lipid pollen macronutrients. Journal of Experimental Biology 219 (24): 3962–3970. DOI:
28. Winston M.L. 1991. The Biology of the Honey Bee. Harvard University Press, Cambridge, USA. 281 pp.






DOI: 10.24425/jppr.2021.136269


Journal of Plant Protection Research; 2021; vol. 61; No 1; 57-62