The use of Allium cepa L. assay as bioindicator for the investigation of genotoxic effects of industrial waste water

Journal title

Archives of Environmental Protection




vol. 47


No 4


Rasgele, Pinar Goc : Duzce University, Faculty of Agriculture, Department of Biosystems Engineering, Duzce, Turkey



Allium cepa assay ; mitotic index ; waste water

Divisions of PAS

Nauki Techniczne




Polish Academy of Sciences


  1. Aksoy, O., Erbulucu, T. & Vatan, E. (2011). Effects of wastewater from olive oil and milk industry on growth and mitosis in Allium cepa root apical meristem. Journal of Applied Biological Sciences, 5,3, pp. 75-78.
  2. Anonymous, (2015). Regulation on the Amendments to the Surface Water Quality Management Regulation (Yüzeysel Su Kalitesi Yönetimi Yönetmeliğinde Değişiklik Yapilmasina Dair Yönetmelik), date of access: 22.05.2021
  3. Aybeke, M., Olgun, G., Sidal, U. & Kolankaya, D. (2000). The effect of olive oil mill effluent on the mitotic cell division and total protein amount of the root tips of Triticum aestivum L. Turkish Journal of Biology, 24, pp. 127-140.
  4. Babic, S., Barisic, J., Visic, H., Klobucar, R.S., Popovic, N.T., Strunjak-Perovic, I., Coz-Rakovac, R. & Klobucar, G. (2017). Embryotoxic and genotoxic effects of sewage effluents in zebrafish embryo using multiple endpoint testing. Water Research, 115, pp. 9-21. DOI: 10.1016/j.watres.2017.02.049
  5. Beyersmann, D. & Hartwig, A. (2008). Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms, Archieves of Toxicology, 82, pp. 493-512. DOI: 10.1007/s00204-008-0313-y
  6. Bianchi, E., Goldoni, A., Trintinaglia, L., Lessing, G., Silva, C.E.M., Nascimento, C.A., Ziulkoski, A.L., Spilki, F.R. & Silva, L.B. (2015). Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil. Brazilian Journal of Biology, 75,2, pp. 68-74. DOI: 10.1590/1519-6984.1913
  7. Butt, M.S., Sharif, K., Bajwa, B.E. & Aziz, A. (2005). Hazardous effects of sewage water on the environment: Focus on heavy metals and chemical composition of soil and vegetables. Management of Environmental Quality: An International Journal, 16, pp. 338-346. DOI 10.1108/14777830510601217
  8. Cavusoglu, K., Yapar, K., Kinalioglu, K., Turkmen, Z., Cavusoglu, K. & Yalcin, E. (2010). Protective role of Ginkgo biloba on petroleum wastewater-induced toxicity in Vicia faba L. (Fabaceae) root tip cells. Journal of Environmental Biology, 31, pp. 319-324.
  9. Darlington, C.A. & La Cour L.E. (1979). The Handling of Chromosomes. 6th ed. Allen and Unwin, London 1979.
  10. El Hajjouji, H., Pinelli, E., Guiresse, M., Merlina, G., Revel, J.C. & Hafidi, M. (2007). Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test. Mutation Research. 634, pp. 25-31. DOI:10.1016/j.mrgentox.2007.05.015
  11. El-Shahaby, O.A., Abdel Migid, H.M., Soliman, M.I. & Mashaly, I.A. (2003). Genotoxicity screening of industrial wastewater using the Allium cepa chromosome aberration assay. Pakistan Journal of Biological Sciences, 6,1, pp. 23-28. DOI: 10.3923/pjbs.2003.23.28
  12. Fiskesjö, G. (1985). The Allium test as a standard in environmental monitoring. Hereditas, 102,1, pp. 99-112. DOI:10.1111/j.1601-5223
  13. Grant, W.F. (1994). The present status of higher plant bioassays for the detection of environmental mutagens. Mutation Research, 310, pp. 175-185. DOI:10.1016/0027-5107(94)90112-0
  14. Kanev, M.O., Ozdemir, K. & Gokalp, F.D. (2017). Evaluation of genotoxic effects on onion (Allium cepa L.) root tip cell of ergene river water. Marmara Journal of Pure and Applied Sciences, 3, pp. 111-117. DOI:10.7240/marufbd.311079
  15. Khan, S., Anas, M. & Malik, A. (2019). Mutagenicity and genotoxicity evaluation of textile industry wastewater using bacterial and plant bioassays. Toxicology Reports, 6, pp. 193-201. DOI:10.1016/j.toxrep.2019.02.002
  16. Levan, A. (1938). The effect of colchicine on root mitoses in Allium. Hereditas, 24,4, pp. 471- 486. DOI:10.1111/j.1601-5223.1938.tb03221.x
  17. Liu, D., Jiang, W., Wang, W. & Zhai, L. (1995). Evaluation of metal ion toxicity on root tip cells by the Allium test, Israel Journal of Plant Sciences, 43: 125-133. DOI:10.1080/07 929978.1995.10676598
  18. Majer, B.J., Grummt, T., Uhi, M. & Knasmuller, S. (2005). Use of plant assays for the detection of genotoxins in the aquatic environment. Acta of Hydrochemistry and Hydrobiology, 33, pp. 45-55. DOI:10.1002/aheh.200300557
  19. Oliveira Júnior, H.M., Sales, P.T.F., Oliveira, D.B., Schimidt, F., Santiago, M.F. & Campos, L.C. (2013). Characterization and genotoxicity evaluation of effluent from a pharmacy industry. AmbiAgua, Taubaté, 8,2, pp. 34-45. DOI:10.4136/ambi-agua1107
  20. Olorunfemi, D., Ogieseri, U.M. & Akinboro, A. (2011). Genotoxicity screening of industrial effluents using onion bulbs (Allium cepa L.). J. Appl. Sci. Environ. Manage, 15,1, pp. 211-216.
  21. Rank, J. & Nielsen, M.H. (1994). Evaluation of the Allium anaphase-telophase test in relation to genotoxicity screening of industrial wastewater. Mutation Research, 312,1, pp. 17-24. DOI:10.1016/0165-1161(94)90004-3
  22. Rasgele, P.G., Kekecoglu, M. & Muranli, F.D.G. (2013). Induction of micronuclei in mice bone marrow cells by cobalt and copper chlorides. Archives of Environmental Protection, 39,1, pp. 75-82. DOI:10.2478/aep-2013-0007.
  23. Solange, B.T. & Haywood, D.L. (2012). Bioindicator of Genotoxicity: The Allium cepa Test, Environmental Contamination, Jatin Kumar Srivastava, IntechOpen, Available from: DOI:10.5772/31371.
  24. Squibb, K.S. & Fowler, B.A. (1981). Relationship between metal toxicity to subcellular systems and the carcinogenic response. Environmental Health Perspectives, 40, pp. 181-188. DOI:10.1289/ehp.8140181
  25. Şık, L., Acar, O. & Aki, C. (2009). Genotoxic effects of industrial wastewater on Allium cepa L. African Journal of Biotechnology, 8,9, pp. 1919-1923.
  26. Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K. & Sutton, D.J. (2012). Heavy metal toxicity and the environment. Molecular, Clinical and Environmental Toxicology, [In:] Luch, A. (ed.), Vol 1: Molecular Toxicology (Experientia Supplementum), Birkhäuser, Berlin, 2012. 133-164. DOI:10.1007/978-3-7643-8340-4_6
  27. Turkez, H., Sisman, T., Incekara, U., Geyikoglu, F., Tatar, A. & Keles, M.S. (2009). The genotoxic and biochemical effects of wastewater samples from a fat plant in Erzurum. Journal of Balıkesir University Institute of Science and Technology, 11,2, pp. 55-63.
  28. Turkmen, Z., Cavusoglu, K., Cavusoglu, K., Yapar, K. & Yalçin, E. (2009). Protective role of Royal Jelly (honeybee) on genotoxicity and lipid peroxidation, induced by petroleum wastewater, in Allium cepa L. root tips. Environmental Technology, 30,11, pp. 1205-1214. DOI:10.1080/09593330903179757






DOI: 10.24425/aep.2021.139497

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