Identification of bacterial species in milk by MALDI-TOF and assessment of some oxidant-antioxidant parameters in blood and milk from cows with different health status of the udder

Journal title

Polish Journal of Veterinary Sciences




vol. 25


No 2


Ozbey, G. : Department of Medical Services and Techniques, Vocational School of Health Services, Firat University, Rectorate Campus, 23119, Elazig, Turkey ; Cambay, Z. : Department of Medical Services and Techniques, Vocational School of Health Services, Firat University, Rectorate Campus, 23119, Elazig, Turkey ; Yilmaz, S. : Department of Biochemistry, Faculty of Veterinary Medicine, Firat University, Rectorate Campus, 23119, Elazig, Turkey ; Aytekin, O. : Department of Medical Services and Techniques, Vocational School of Health Services, Firat University, Rectorate Campus, 23119, Elazig, Turkey ; Zigo, F. : Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 04001, Slovakia ; Ozçelik, M. : Department of Medical Services and Techniques, Vocational School of Health Services, Firat University, Rectorate Campus, 23119, Elazig, Turkey ; Otlu, B. : Department of Medical Microbiology, Faculty of Medicine, Inonu University, Main Campus, 44280, Battalgazi, Malatya, Turkey



dairy cows ; CMT ; mastitis ; malondialdehyde ; glutathione ; MALDI-TOF

Divisions of PAS

Nauki Biologiczne i Rolnicze




Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn


Abdel-Hamied E, Mahmoud MM (2020) Antioxidants profile, oxidative stress status, leukogram and selected biochemical indicators in dairy cows affected with mastitis. J Anim Health Product 8: 183-188.
Amer S, Gálvez FL, Fukuda Y, Tada C, Jimenez, IL, Valle WF, Nakai Y (2018) Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador. J Vet Med Sci 80: 861-868.
Andrei S, Matei S, Fit N, Cernea C, Ciupe S, Bogdan S, Groza IS (2011) Glutathione peroxidase activity and its relationship with somatic cell count, number of colony forming units and protein content in subclinical mastitis cow’s milk. Romanian Biotechnological Letters 16: 6209-6217.
Bexiga R, Rato MG, Lemsaddek A, Semedo-Lemsaddek T, Carneiro C, Pereira H, Mellor DJ, Ellis KA, Vilela CL (2014) Dynamics of bovine intramammary infections due to coagulase-negative staphylococci on four farms. J Dairy Res 81: 208-214.
Cameron M, Perry J, Middleton JR, Chaffer M, Lewis J, Keefe GP (2018) Short communication: Evaluation of MALDI-TOF mass spectrometry and a custom reference spectra expanded database for the identification of bovine-associated coagulase-negative staphylococci. J Dairy Sci 101: 590-595.
Carvalho-Sombra TC, Fernandes DD, Bezerra BM, Nunes-Pinheiro DC (2021) Systemic inflammatory biomarkers and somatic cell count in dairy cows with subclinical mastitis. Vet Anim Sci 11: 100165.
Castillo C, Hernández J, Valverde I, Pereira V, Sotillo J, López Alonso ML, Benedito JL (2006) Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Res Vet Sci 80: 133-139.
Chakraborty S, Dhama K, Tiwari R, Yatoo MI, Khurana SK, Khandia R (2019) Technological interventions and advances in the diagnosis of intramammary infections in animals with emphasis on bovine population – a review. Vet Q 39: 76-94.
Celi P (2011) Biomarkers of oxidative stress in ruminant medicine. Immunopharmacol Immunotoxicol 33: 233-240.
Condas LA, De Buck J, Nobrega DB, Carson DA, Roy JP, Keefe GP, DeVries TJ, Middleton JR, Dufour S, Barkema HW (2017) Distribution of nonaureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis. J Dairy Sci 100: 5613-5627.
De Visscher A, Piepers S, Haesebrouck F, De Vliegher S (2016) Teat apex colonization with coagulase-negative Staphylococcus species before parturition: distribution and species-specific risk factors. J. Dairy Sci 99: 1427-1439.
Dubois D, Grare M, Prere MF, Segonds C, Marty N, Oswald E (2012) Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. J Clin Microbiol 50: 2568-2576.
Dufour S, Munoz M (2018) Milk bacteriological analysis using MALDI-TOF technology. Carol Hulland, University of Wisconsin-Madison, Version. pp 1-5; available on:
Garcia AB, Shalloo L (2015) Invited review: the economic impact and control of paratuberculosis in cattle. J Dairy Sci 98: 5019-5039.
Goff JP, Horst RL (1997) Physiological changes at parturition and their relationship to metabolic disorders. J Dairy Sci 80: 1260-1268.
Huijps K, Lam TJ, Hogeveen H (2008) Costs of mastitis: facts and perception. J Dairy Res 75:113-120.
Jackson P, Cockcroft P (2002) Clinical examination of farm animals. Blackwell Science Ltd Oxford, UK, ISBN 0-632-05706-8, pp 154-166.
Kassim A, Pflüger V, Premji Z, Daubenberger C, Revathi G (2017) Comparison of biomarker based Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and conventional methods in the identification of clinically relevant bacteria and yeast. BMC Microbiol 17: 128.
Kaya E, Yılmaz S, Ceribasi S (2019) Protective role of pro polis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats. J Vet Res 63: 423-431.
Kırkan S, Parın U, Tanır T, Yuksel HT (2018) Identification of the Staphylococcus species which cause cattle mastitis using MALDI-TOF MS. Appr Poult Dairy & Vet Sci 4: 317-323.
Ma J-Q, Liu C-M, Qin Z-H, Jiang J-H, Sun Y-Z (2011) Ganoderma applanatum terpenes protect mouse liver against benzo(α)pyren-induced oxidative stress and inflamma tion. Environ Toxicol Pharm 31: 460-468.
Mahapatra A, Panigrahi S, Patra RC, Rout M, Ganguly S (2018) A study on bovine mastitis related oxidative stress along with therapeutic regimen. Int J Curr Microbiol Appl Sci 7: 247-256.
Malinowski E, Lassa H, Kłossowska A, Smulski S, Markiewicz H, Kaczmarowski M (2006) Etiological agents of dairy cows’ mastitis in western part of Poland. Pol J Vet Sci 9: 191-194.
Mørk T, Jørgensen HJ, Sunde M, Kvitle B, Sviland S, Waage S, Tollersrud T (2012) Persistence of staphylococcal species and genotypes in the bovine udder. Vet Microbiol 159: 171-180.
Mpatswenumugabo JP, Bebora LC, Gitao GC, Mobegi VA, Iraguha B, Kamana O, Shumbusho B (2017) Prevalence of subclinical mastitis and distribution of pathogens in dairy farms of Rubavu and Nyabihu Districts, Rwanda. J Vet Med 2017: 8456713.
Ndahetuye JB, Persson Y, Nyman A-K, Tukei M, Ongol MP, Båge R (2019) Aetiology and prevalence of subclinical mastitis in dairy herds in peri-urban areas of Kigali in Rwanda. Trop Anim Health Prod 51: 2037-2044.
Oliveira L, Hulland C, Ruegg PL (2013) Characterization of clinical mastitis occurring in cows on 50 large dairy herds in Wisconsin. J Dairy Sci 96: 7538-7549.
Ozbey G, Otlu B, Yakupogullari Y, Celik B, Tanriverdi ES, Kelestemur N, Safak T, Risvanli A, Persad A, Sproston E (2022) Investigation of bacterial pathogens in milk from mastitic dairy cattle by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Thai J Vet Med 52: 63-73.
Pascu C, Herman V, Iancu I, Costinar L (2022) Etiology of mastitis and antimicrobial resistance in dairy cattle farms in the Western Part of Romania. Antibiotics (Basel) 11: 57.
Sharifi S, Pakdel A, Ebrahimi M, Reecy JM, Farsani SF, Ebrahimie E (2018) Integration of machine learning and meta-analysisidentifies the transcriptomic bio-signature of mastitis disease in cattle. PloS One 22: 13 e0191227.
Sharma N, Jeong DK (2013) Stem cell research: a novel boulevard towards improved bovine mastitis management. Int J Biol Sci 9: 818-829.
Sharma N, Singh NK, Singh OP, Pandey V, Verma PK (2011) Oxidative stress and antioxidant status during transition period in dairy cows. Asian-AJAS 24: 479-484.
Sharma L, Verma AK, Rahal A, Kumar A, Nigam R (2016) Relationship between serum biomarkers and oxidative stress in dairy cattle and buffaloes with clinical and sub clinical mastitis. Biotechnology 15: 96-100.
Shell WS, Sayed ML, El-Gedawy AA, El Sadek GM, Samy AA, Ali AMM (2017) Identification of Staphylococcus aureus causing bovine mastitis using MALDI -TOF fingerprinting. Int J Dairy Sci 12: 105-113.
Simsek H, Aksakal M (2005) The effect of vitamin E on lipid peroxidation and some antioxidants in blood and milk of cows with subclinical mastitis. Ankara Üniv Vet Fak Derg 52: 71-76.
Simsek H, Aksakal M (2006) The effect of vitamin E on levels of vitamin A, beta carotene, glutahione peroxidase and reduced glutathione in plasma and vitamin A in milk in cows with subclinical mastitis. F.Ü. Sağlık Bil. Dergisi 20: 199-203.
Singhal N, Kumar M, Kanaujia PK, Virdi JS (2015) MALDI -TOF mass spectrometry: An emerging technology for microbial identification and diagnosis. Front Microbiol 6: 791.
Supré K, Haesebrouck F, Zadoks RN, Vaneechoutte M, Piepers S, De Vliegher S (2011) Some coagulase-negative Staphylococcus species affect udder health more than others. J Dairy Sci 94: 2329-2340.
Suriyasathaporn W, Chewonarin T, Vinitketkumnuen U (2012) Differences in severity of mastitis and the pathogens causing various oxidative product levels. Adv Biosci Biotechnol 3: 454-458.
Sztachańska M, Barański W, Janowski T, Pogorzelska J, Zduńczyk S (2016) Prevalence and etiological agents of subclinical mastitis at the end of lactation in nine dairy herds in North-East Poland. Pol J Vet Sci 19: 119-124.
Taponen S, Liski E, Heikkilä A-M, Pyörälä S (2017) Factors associated with intramammary infection in dairy cows caused by coagulase-negative staphylococci, Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, Corynebacterium bovis, or Escherichia coli. J Dairy Sci 100: 493-503.
Turk R, Koledic M, Macesic N, Benic M, Dobranic V, Duricic D, Cvetnič L, Samardzija M (2017) The role of oxidative stress and inflammatory response in the pathogenesis of mastitis in dairy cows. Mljekarstvo 67: 91-101.
Ustuner MA, Kaman D, Colakoglu N (2017) Effects of benfotiamine and coenzyme Q10 on kidney damage induced gentamicin. Tissue Cell 49: 691-696.
Vanderhaeghen W, Vandendriessche, S, Crombé F, Nemeghaire S, Dispas M, Denis O, Hermans K, Haesebrouck F, Butaye P (2013) Characterization of methicillin-resistant non-Staphylococcus aureus staphylococci carriage isolates from different bovine populations. J Antimicrob Chemother 68: 300-307.
Vasiľ M, Zigo F, Farkašová Z, Pecka-Kielb E, Bujok J, Illek J (2022) Comparison of effect of parenteral and oral supplementation of Selenium and vitamin E on selected antioxidant parameters and udder health of dairy cows. Pol J Vet Sci 25: 155-164.
Viguier C, Arora S, Gilmartin N, Welbeck K, O’Kennedy R (2009) Mastitis detection: Current trends and future perspectives. Trends Biotechnol 27: 486-493.
Waller KP (2000) Mammary gland immunology around parturition. Influence of stress, nutrition and genetics. Adv Exp Med Biol 480: 231-245.
Webster J (2020) Understanding the dairy cows. 3rd ed. Oxford, UK: Wiley Blackwell, p 258.
Zajac P, Tomaska M, Murarova A, Capla J, Curlej J (2012) Quality and safety of raw cow’s milk in Slovakia in 2011. Potravinarstvo Slovak J Food Sci 6: 64-73.
Zeryehun T, Abera G (2017) Prevalence and bacterial Isolates of mastitis in dairy farms in selected districts of Eastern Harrarghe Zone, Eastern Ethiopia. J Vet Med 2017: 6498618.
Zigo F, Elecko J, Vasil M, Ondrasovicova S, Farkasova Z, Malova J, Takac L, Zigova M, Bujok J, Pecka-Kielb E, Timkovicova-Lackova P (2019a) The occurrence of mastitis and its effect on the milk malondialdehyde concentrations and blood enzymatic antioxidants in dairy cows. Vet Med 64: 423-432.
Zigo F, Elečko J, Farkašová Z, Zigová M, Vasil M, Ondrašovičová S, Kudelkova L (2019b) Preventive methods in reduction of mastitis pathogens in dairy cows. J Microbiol Biotechnol Food Sci 9: 121-126.
Zigo F, Elečko J, Vasil’ M, Farkasová Z, Zigová M, Takáč L, Takáčová J, Bujok J, Kielb E (2019c) Assessment of lipid peroxidation in dairy cows with subclinical and clinical mastitis. Potravinarstvo Slovak J Food Sci 13: 244-250.
Zigo F, Elecko J, Vasil M, Farkasova Z, Zigova M, Takac L, Takacova J (2019d) Etiology of mastitis in herds of dairy cows and ewes situated in marginal parts of Slovakia. EC Vet Sci 4: 72-80.
Zigo F, Farkašová Z, Výrostková J, Regecová I, Ondrašovičová S, Vargová M, Sasáková N, Pecka -Kielb E, Bursová Š, Kiss DS (2022) Dairy cows’ udder pathogens and occurrence of virulence factors in staphylococci. Animals (Basel) 12: 470.
Zigo F, Vasil’ M, Ondrašovičová S, Výrostková J, Bujok J, Pecka-Kielb E (2021) Maintaining optimal mammary gland health and prevention of mastitis. Front Vet Sci 8: 607311.






DOI: 10.24425/pjvs.2022.141811