Details

Title

Workers of honey bee (Apis mellifera L.) reared in small-cell combs in apiary conditions show higher activity of the proteolytic system and lower protein concentrations on the cuticle surface than workers reared in standard-cell combs

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2025

Volume

vol. 28

Issue

No 1

Authors

Affiliation

Dziechciarz, P. : Subdepartment of Apidology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland ; Strachecka, A. : Department of Invertebrate Ecophysiology and Experimental Biology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland ; Borsuk, G. : Subdepartment of Apidology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland ; Olszewski, K. : Subdepartment of Apidology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland

Keywords

small-cell combs ; Apis mellifera ; body surface proteins ; proteolytic enzymes ; protease inhibitors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Coverage

111–122

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliography

Anson ML (1938) The estimation of pepsin, tripsin, papain and cathepsin with hemoglobin. J Gen Physio 22: 79-89.

Berry JA, Owens WB, Delaplane KS (2010) Small-cell comb foundation does not impede Varroa mite population growth in honey bee colonies. Apidologie 41: 40-44.

Blacquière T, Smagghe G, Van Gestel CA, Mommaerts V (2012) Neonicotinoids in bees: a review on concentrations, sideeffects and risk assessment. Ecotoxicology 21: 973-992.

Bode W, Fernandez‐Catalan C, Nagase H, Maskos K (1999)Endoproteinase‐protein inhibitor interactions. APMIS107: 3-10.

Coffey MF, Breen J, Brown MJ, McMullan JB (2010) Brood-cell size has no influence on the population dynamicsof Varroa destructor mites in the native western honey bee, Apis mellifera mellifera. Apidologie 41: 522-530.

Crailsheim K (1986) Dependence of protein metabolism on age and season in the honeybee (Apis mellifica carnica Pollm). J Insect Physiol 32: 629-634.

Crailsheim K, Schneider LH, Hrassnigg N, Bühlmann G,Brosch U, Gmeinbauer R, Schöffmann B (1992) Pollenconsumption and utilization in worker honeybees (Apismellifera carnica): Dependence on individual age and function. J Insect Physiol 38: 409-419.

Cremer S, Armitage SA, Schmid-Hempel P (2007) Socialimmunity. Curr Biol 17: R693-702.

Cullen MG, Thompson LJ, Carolan JC, Stout JC, Stanley DA (2019) Fungicides herbicides and bees: A systematic review of existing research and methods. PLoS One 14: e0225743

da Luz GF, Santana WC, Santos CG, Santana LM, Serrão JE (2022) Cuticle melanization and the expression of immune-related genes in the honeybee Apis mellifera (Hymenoptera: Apidae) adult workers. CBPB 257: 110679.

Dziechciarz P, Borsuk G, Olszewski K (2022b) Dead brood of Apis mellifera is removed more effectively from small-cell combs than from standard-cell combs. Animals (Basel)12: 418.

Dziechciarz P, Borsuk G, Olszewski K (2021) Possibilityto change the body size in worker bees by a combinationof small-cell and standard-cell combs in the same nest.Apidologie 52: 1017-1032.

Dziechciarz P, Strachecka A, Borsuk G, Olszewski K (2023b) Effect of Rearing in Small-Cell Combs on Activitiesof Catalase and Superoxide Dismutase and Total Antioxidant Capacity in the Hemolymph of Apis melliferaWorkers. Antioxidants (Basel) 12: 709.

Dziechciarz P, Strachecka A, Borsuk G, Olszewski K (2023a) Workers of Apis mellifera Reared in Small-Cell Combs Show Higher Activity of the Proteolytic System in Hemolymph than Workers Reared in Standard-Cell Combs in Laboratory Cage Tests. Animals (Basel) 13: 1368.

Dziechciarz P, Strachecka A, Olszewski K (2022a) Effect of Comb Cell Width on the Activity of the Proteolytic System in the Hemolymph of Apis mellifera Workers. Animals(Basel) 12: 978.

Eckholm BJ, Huang MH, Anderson KE, Mott BM, DeGrandi-Hoffman G (2015) Honey bee (Apis mellifera) intracolonial genetic diversity influences worker nutritional status. Apidologie 46: 150-163.

Ellis AM, Hayes GW, Ellis JD (2009) The efficacy of small cell foundation as a varroa mite (Varroa destructor) control. Exp Appl Acarol 47: 311-316.

Evans JD, Aronstein K, Chen YP, Hetru C, Imler JL, Jiang H, Kanost M, Thompson GJ, Zou Z, Hultmark D (2006)Immune pathways and defence mechanisms in honey bees Apis mellifera. Insect Mol Biol 15: 645-656.

Evans JD, Spivak M (2010) Socialized medicine: individual and communal disease barriers in honey bees. J Invertebr Pathol 103: S62-S72.

Fluri P, Lüscher M, Wille H, Gerig L (1982) Changes in weight of the pharyngeal gland and haemolymph titres of juvenile hormone, protein and vitellogenin in worker honey bees.J Insect Physiol 28: 61-68.

Huo X, Wu B, Feng M, Han B, Fang Y, Hao Y, Meng L,Wubie AJ, Fan P, Hu H, Qi Y, Li J (2016) Proteomic Analysis Reveals the Molecular Underpinnings of Mandibular Gland Development and Lipid Metabolism in Two Linesof Honeybees (Apis mellifera ligustica). J Proteome Res15: 3342-3357.

Kanost MR, Clarke T (2005) Proteases In: Gilbert LI, Gill S (Eds) Comprehensive Molecular Insect Science. 1st ed.,Elsevier: Amsterdam Netherlands pp 247-266.

Lee TM, Lin YH (1995) Trypsin inhibitor and trypsin – likeprotease activity in air – or submergence – grown rice(Oryza sativa L) coleoptiles. Plant Sci 106: 43-54.

Leung D, Abbenante G, Fairlie DP (2000) Protease inhibitors: current status and future prospects. J Med Chem43: 305-341.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.

Łoś A, Strachecka A (2018) Fast and Cost-Effective Biochemical Spectrophotometric Analysis of Solution of Insect “Blood” and Body Surface Elution. Sensors (Basel)18: 1494.

Maggi M, Damiani N, Ruffinengo S, De Jong D, Principal J, Eguaras M (2010) Brood cell size of Apis mellifera modifies the reproductive behavior of Varroa destructor. Exp Appl Acarol 50: 269-279.

McMullan JB, Brown MJ (2006) The influence of small-cell brood combs on the morphometry of honeybees (Apismellifera). Apidologie 37: 665-672.

Message D, Goncalves LS (1995) Effect of the size of worker brood cells of Africanized honey bees on infestation andof the ectoparasitic mite Varroa Jacobsoni Oud. Apidologie 26: 381-386.

Migdał P, Murawska A, Strachecka A, Bieńkowski P, Roman A (2021) Honey Bee Proteolytic System and Behavior Parameters under the Influence of an Electric Field at 50 Hz and Variable Intensities for a Long Exposure Time. Animals (Basel) 11: 863.

Olszewski K, Paleolog J (2005) Foraging and hoarding efficiency in Buckfast purebreds and Norwegian Black Bee(A. m. mellifera) hybrids. Part. 1. Annual honey yield versus results of field flying cage and laboratory tests. J Apic Sci 49: 17-25.

Olszewski K, Borsuk G, Paleolog J, Strachecka A (2014b) Life span of worker honeybees reared in colonies kept on small-cell combs. Med Weter 70: 777-780.

Olszewski K, Borsuk G, Paleolog J, Strachecka A, Bajda M (2014a) Hygienic behaviour of colonies kept on small-cell combs. Med Weter 70: 774-776.

Paleolog J, Wilde J, Siuda M, Bąk B Wójcik Ł, Strachecka A (2020) Imidacloprid markedly affects hemolymph proteolysis biomarkers DNA global methylation and the cuticleproteolytic layer in western honeybees. Apidologie51: 620-630.

Piccirillo GA, De Jong D (2003) The influence of brood comb cell size on the reproductive behavior of the ectoparasitic mite Varroa Destructor in Africanized honey bee colonies. Genet Mol Res 2: 36-42.

Schacterle GR, Pollack RL (1973) A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem 51: 654-655.

Seeley T, Griffin S (2011) Small-cell comb does not controlVarroa mites in colonies of honeybees of European origin. Apidologie 42: 526-532.

Singer HJ, van Praagh JP, Paulus HF (2019) Interactionsbetween honeybees and Varroa mites influenced by cellsizes and hygienic behavior. Entomol Gener. 38: 255-273.

Skowronek P, Wójcik Ł, Strachecka A (2021) Cannabis Extract Has a Positive – Immunostimulating Effect through Proteolytic System and Metabolic Compounds of Honey Bee (Apis mellifera) Workers, Animals (Basel) 11: 2190.

Strachecka A, Demetraki-Paleolog J (2011) The body surface proteolytic system of apis mellifera in preserving the health of bee colonies. Kosmos 60: 43-51.

Strachecka A, Grzywnowicz K (2008) Activity of proteaseinhibitors on the body surface of the honeybee. Med Weter 64: 1256-1259.

Strachecka A, Łoś A, Filipczuk J, Schulz M (2018) Individual and social immune mechanisms of the honey bee. Med Weter 74: 426-433.

Strachecka A, Olszewski K, Kuszewska K, Chobotow J,Wójcik Ł, Paleolog J, Woyciechowski M (2021) Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials Sci Rep 11: 13887.

Strachecka A, Olszewski K, Paleolog J (2015) Curcumin stimulates biochemical mechanisms of Apis mellifera resistance and extends the apian life-span. J Apic Sci 59: 129-141.

Strachecka A, Olszewski K, Paleolog J (2016) Varroa treatment with bromfenvinphos markedly suppresses honeybee biochemical defence levels. Entomol Exp Appl. 160: 57-71.

Strachecka A, Olszewski K, Paleolog J, Borsuk G, Bajda M, Krauze M, Merska M, Chobotow J (2014) Coenzyme Q10 treatments influence the lifespan and key biochemical resistance systems in the honeybee Apis mellifera. Arch Insect Biochem Physiol 86: 165-179.

Strachecka A, Paleolog J, Olszewski K, Borsuk G (2012)Influence of Amitraz and Oxalic Acid on the CuticleProteolytic System of Apis mellifera L. Workers. Insects3: 821-832.

Tautz J (2007) Phänomen Honigbiene. 2nd ed., Spectrum-Elsevier: Heidelberg Germany, p 156.

Taylor MA, Goodwin RM, McBrydie HM, Cox HM (2008)The effect of honey bee worker brood cell size on Varroa destructor infestation and reproduction. J Apic Res.47: 239-242.

Wilson-Rich N, Dres ST, Starks PT (2008) The ontogenyof immunity: development of innate immune strengthin the honey bee (Apis mellifera). J Insect Physiol54: 1392-1399.

Winston ML (1987) The Biology of the Honey Bee. Havard University Press: Cambridge, UK

Date

20.03.2025

Type

Article

Identifier

DOI: 10.24425/pjvs.2025.154019
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