Life Sciences and Agriculture

Polish Journal of Veterinary Sciences

Content

Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 1

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Abstract

The aim of this study was to investigate the cardiotoxic effect of the combination of tilmicosin and diclofenac sodium in sheep. Thirty-two sheep were used and were randomly divided into four equal groups as tilmicosin (T), diclofenac sodium (D), tilmicosin+diclofenac sodium (TD) and control (C) group. Group T received a single dose of tilmicosin, Group D was administered diclofenac sodium once a day for 3 days, and group TD was administered diclofenac and tilmicosin at the same doses as group T and D. Group C received NaCl in a similar way. The blood samples were taken before dosing and at 4th, 8th, 24th and 72nd hour post-dosing for measurement of cardiac markers such as H-FABP, cTn-I, CK-MB. H-FABP level of group TD was found to be significantly (p<0.05) higher than of group C at the 8th, 24th and 72nd hour and group D and T at the 72nd hour. cTn-I and CK-MB levels of group TD were found significantly (p<0.05) higher compared with other groups. In conclusion, the combined use of tilmicosin and diclofenac in sheep causes an increase in cardiac biomarkers and it can be stated that this combination of drugs may cause cardiac damage.
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Authors and Affiliations

R. Yildiz
1
D. Durna Corum
2
O. Corum
2
M. Ider
3
O. Atik
4
M. Ok
3
K. Uney
5

  1. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Burdur Mehmet Akif Ersoy, 15030, Burdur, Turkey
  2. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal, 31060, Hatay, Turkey
  3. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Selcuk, 42100, Konya, Turkey
  4. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Afyon Kocatepe, 03030, Afyonkarahisar, Turkey
  5. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, 42100, Konya, Turkey
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Abstract

Chlorocresol nanoemulsion disinfectant (CND) is an environmental disinfectant prepared with nanoemulsion as its drug carrier. This study aimed to investigate the bactericidal effect of CND on Staphylococcus aureus ( S. aureus) and its effect on bacterial ultrastructure. The neutralizing effect of CND against S. aureus was first screened by suspension quantitative evaluation experiment procedure of neutralizer. Disinfection performance was evaluated by the determination of Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), quantitative bactericidal experiment, and comparative experiment of disinfection performance between 0.1% CND and 0.1% chlorocresol aqueous solution. Meanwhile, the effect of CND on the ultrastructure of S. aureus was investigated with scanning electron microscope (SEM) and transmission electron microscope (TEM) to preliminarily explore the bactericidal mechanism. The results showed that 3% Tween-80 in PBS could be screened as the neutralizer of CND against S. aureus. MIC and MBC were 100 μg/mL and 200 μg/mL, respectively. The bactericidal rates were all 100% when 0.06% and 0.08% disinfectant acted for 15 and 5 min, respectively. Furthermore, compared with 0.1% chlorocresol aqueous solution, the bactericidal effect of 0.1% CND was significantly enhanced (p<0.01). After treatment with CND for 10 min, SEM observation showed that the morphology of S. aureus cells were changed and the integrity destroyed. TEM observation showed that the cell shape changed, and the structures of the cell wall, cell membrane and cytoplasm were damaged in varying degrees. CND showed the strong bactericidal effect on S. aureus and could cause ultrastructure alterations of S. aureus.
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Authors and Affiliations

Y.F. Zhang
1
Y.W. Sun
1
X.H. Liu
1
Z.X. An
1
X.F. Yang
1

  1. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Hualan Road No. 90, Xinxiang City, Henan Province, 453003, China
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Abstract

To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
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Authors and Affiliations

A. Lin
1
X. Hu
1
S. Cui
1
T. Yang
1
Z. Zhang
1
P. Li
1
M. Guo
1
Y. Lu
1

  1. College of Life Sciences and Resource Environment, Yichun University, No 576, Xuefu Road, Yuanzhou district, Yichun, Jiangxi, 336000, China
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Abstract

Blood samples from forty-six roe deer ( Capreolus capreolus) acquired during officially approved hunting in six hunting divisions throughout Poland were used to isolate the genomic DNA. All individuals were genotyped by MD_Bovine BeadChip (Illumina) for 46.750 Single Nucleotide Polymorphism (SNP) markers. SNPs of inappropriate clusters, with a marker call rate lower than 90% and with a minor allele frequency (MAF) lower than 0.01, located on sex chromosomes and mitochondrial DNA, were removed. Altogether, 21.033 SNP markers were included for further analysis. Observed and expected heterozygosity amounted to 0.098 and 0.119, respectively. Among 21.033 markers, a panel of 148 SNPs were selected for relationship analysis. They were unlinked and had a MAF higher than 0.2. This set of SNPs showed a probability of parentage exclusion of 1.29x10 -6 and 2.37x10 -19 for one, and two known parents, respectively. The probability of identity was estimated at 1.8x10 -40. The probabilities obtained in this study are sufficient for the monitoring and effective management of the genetic diversity of roe deer in Poland and is a cost-effective complementary tool for forensic applications.
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Authors and Affiliations

K. Oleński
1
D. Zalewski
2
S. Kamiński
1

  1. University of Warmia and Mazury, Department of Animal Genetics, M. Oczapowskiego 5, 10-718 Olsztyn, Poland
  2. University of Warmia and Mazury, Department of Fur-bearing Animal Breeding and Game Management, M. Oczapowskiego 5, 10-718 Olsztyn, Poland
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Abstract

In this study, the presence and level of macrolide group antibiotics (tylosin and tilmicosin) were analyzed by the High-Performance Liquid Chromatography (HPLC) method in a total of 126 raw meat samples, including 42 chicken breast and 84 beef neck, available for consumption in the Burdur province (Turkey). The method demonstrated good linearity (R2 > 0.999) over the assayed concentration range (0.10-10 μg/mL). Intra-day and inter-day recoveries were used to express the accuracy of the method at three different levels of 0.5, 1, 2.5 μg/mL. Intraday recoveries and relative standard deviation values ranged from 97.270 (0.054)% to 98.643 (0.061)%, and inter-day recoveries and relative standard deviation values ranged from 97.057 (0.070)% to 98.197(0.042)% for tylosin. Intraday recoveries and relative standard deviation values ranged from 96.360 (0.065)% to 98.153 (0.046)%, and inter-day recoveries and relative standard deviation values ranged from 96.050 (0.058)% to 97.053 (0.096)% for tilmicosin. The limit of detection (LOD) value was calculated as 0.473 μg/kg for tylosin, and 0.481 μg/kg for tilmicosin; the limit of quantification (LOQ) value was calculated as 1.561 μg/kg for tylosin, and 1.587 μg/kg for tilmicosin. In general, tylosin and tilmicosin were determined in the range of 8-256 μg/kg and 30-447 μg/kg, respectively, in chicken breast meat samples; also, they were detected in the range of 36-1209 μg/kg and 30-1102 μg/kg, respectively, in beef neck meat samples. It was also found that the residues of tylosin and tilmicosin in chicken and beef meats from the market were at a much higher level than the acceptable limits specified in the regulations. This creates serious problems in terms of the ecosystem, food technology, and public health, and causes significant economic losses.
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Authors and Affiliations

M. Gürel Yücel
1
H. Seçilmiş
2
F. Taşçı
1

  1. Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, 15030, Istiklal Campus, Burdur, Turkey
  2. Department of Chemistry, Faculty of Arts and Science, Burdur Mehmet Akif Ersoy University, 15030, Istiklal Campus, Burdur, Turkey
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Abstract

Members of Enterobacteriaceae are known to produce extended-spectrum beta-lactamases (ESBL) which hydrolyze the beta-lactam group of antibiotics. The existence of ESBL-producing Salmonella enterica ( S. enterica) and Escherichia coli ( E. coli) harbored by urban avifauna was investigated in this study. Dropping samples (n= 180) were collected from six different bird species in the district Jhang, Punjab province, Pakistan. Isolation and identification of ESBL isolates were made by using cefotaxime- (4 mg/L) supplemented MacConkey agar and double disc synergy test (DDST). Polymerase chain reaction (PCR) was performed for the detection of four different ESBL genes including bla CTX-M, bla TEM, bla SHV and bla OXA. A total of 42.69% isolates were confirmed as ESBL via DDST including 30.64% S. enterica and 49.54% E. coli. The incidence of ESBL S. enterica and ESBL E. coli was found highest in egret ( Ardea alba) and pigeon ( Columba livia) as 64.28% and 78.95%, respectively. The bla CTX-M gene was detected in 57.89% and 64.81% of isolates of S. enterica and E. coli, respectively. Among other genes in S. enterica and E. coli, bla TEM (21.05%, 20.4%); bla SHV (15.78%, 9.26%), and bla OXA (5.26%, 5.56%) were detected, respectively. All of the tested isolates were found resistant to at least one of the thirteen antimicrobial agents except meropenem. To the best of our knowledge, this is the first study reporting the incidence and genetic diversity of ESBL bacteria associated with urban avifauna in Pakistan. The urban avifauna can serve as a potential subject of bio-surveillance to monitor the emergence of antimicrobial-resistant bacteria.
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Authors and Affiliations

M.A. Saeed
1
U. Waheed
1
S. Ehtisham-ul-Haque
1
A.U. Khan
1
M. Kashif
2
M.F. Qamar
1
A. Ghafoor
3
M. Saqlain
1
J. Asghar
1

  1. Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, CVAS Campus, 12-Km Chiniot Road, Jhang, 35200, Pakistan
  2. Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, CVAS Campus, 12-Km Chiniot Road, Jhang, 35200, Pakistan
  3. Institute of Microbiology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, 54000, Pakistan
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Abstract

Nontuberculous mycobacteria (NTM) have recently emerged as important bacterial pathogens of animals and humans. Of particular concern is the high level of antimicrobial resistance displayed by these organisms, which complicates treatment and potential successful outcomes. Here, we evaluated the potential of Carlina acaulis L. as a source of novel anti-mycobacterial agents. Our goal was to measure the activity of aqueous, ethanol, and chloroform C. acaulis root extracts against 99 NTM strains. GC-MS spectroscopy analyses were performed to deliver qualitative and quantitative data on the composition of C. acaulis extract. In our study, we have shown for the first time the activity of C. acaulis extracts against NTM. The highest activity was exhibited by the chloroform extract, which inhibited the growth of more than 90% of the strains at the dose of 100 μg/mL (MIC90 = 100 μg/mL). The results of the GC-MS analysis of the C. acaulis chloroform extract contributed to the identification of 37 compounds, with carlina oxide as the most representative compound (69.52%) followed by 3,4-dihydro-2H-phenanthren- -1-one (6.54%) and stigmast-5-en-3-ol (4.14%). Our results indicate that C. acaulis chloroform and ethanol extracts have potential for treatment of NTM infections and that this plant contains anti-mycobacterial compounds.
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Authors and Affiliations

K. Puk
1
J. Wawrzykowski
2
L. Guz
1

  1. Department of Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland
  2. Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Science in Lublin, Akademicka 12, 20-033 Lublin, Poland
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Abstract

Mature males of a wild boar-pig crossbreed, during the long and short day season, were used for the study which demonstrates that the chemical light carrier CO regulates the expression of biological clock genes in the hypothalamus via humoral pathways. Autologous blood with experimentally elevated concentrations of endogenous CO (using lamps with white light-emitting diodes) was infused into the ophthalmic venous sinus via the right dorsal nasal vein. Molecular biology methods: qPCR and Western Blot were used to determine the expression of genes and biological clock proteins. The results showed that elevated endogenous CO levels, through blood irradiation, induces changes in genes expression involved in the functioning of the main biological clock located in suprachiasmatic nuclei. Changes in the expression of the transcription factors Bmal1, Clock and Npas2 have a similar pattern in both structures, where a very large decrease in gene expression was shown after exposure to elevated endogenous CO levels. The changes in the gene expression of PER 1-2, CRY 1-2, and REV-ERB α-β and ROR β are not the same for both POA and DH hypothalamic structures, indicating that both structures respond differently to the humoral signal received.
The results indicate that CO is a chemical light molecule whose production in an organism depends on the amount of light. An adequate amount of light is an essential factor for the proper functioning of the main biological clock.
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Authors and Affiliations

P. Gilun
1
M. Koziorowska-Gilun
2
B. Wąsowska
1
M. Sowa-Kućma
3
K. Kozioł
4
M. Romerowicz-Misielak
4
W. Kordan
2
M. Koziorowski
4

  1. Department of Local Physiological Regulations, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748, Olsztyn, Poland
  2. Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland
  3. Department of Human Physiology, Institute of Medical Sciences, Medical College of Rzeszow University, Kopisto 2a, Rzeszow, 35-959, Poland
  4. Department of Animal Physiology and Reproduction, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland
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Abstract

Zoonoses are frequently associated with wild animals. Research on reptiles either living in their natural habitat or kept as pet animals has shown that these animals frequently serve as the asymptomatic hosts of bacterial zoonotic agents, including Salmonella spp. and Escherichia coli. Studies have shown the potential of reptiles to transmit these pathogens to humans and other animals. Epidemiological research on the herpetofauna of various regions has demonstrated the high potential of reptiles as a reservoir of Salmonella spp. In the present study, Salmonella spp. were not isolated or identified from the snake-eyed lizard. Out of 150 cloacal swab samples of snake-eyed lizard 25 (16.7%) E. coli were isolated and out of these 4 (2.7%) were identified to be E. coli O157:H7 by PCR. The results suggest that Ophisops elegans could be involved in the transmission of E. coli, rather than Salmonella spp. This study demonstrates for the first time that the snake-eyed lizard acts as a cloacal carrier of E. coli O157:H7 and presents data that may aid in preventing the transmission of this strain to humans.
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Authors and Affiliations

S. Tarhane
1
E. Bozkurt
1
F. Büyük
2

  1. Veterinary Department, Eldivan Vocational School of Health Services, Çankırı Karatekin University, 18100, Çankırı, Turkey
  2. Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey
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Abstract

The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 μM, LUT 50 μM, LUT 100 μM and LUT 200 μM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 106 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 μM compared to the other groups (p<0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 μM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H2O2 percentage was found to be lower in all experimental groups compared to the control group (p<0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.
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Yan Y, Jun C, Lu Y, Jiangmei S (2019) Combination of metformin and luteolin synergistically protects carbon tetrachloride‐induced hepato-toxicity: Mechanism involves antioxidant, anti‐inflammatory, antiapoptotic, and Nrf2/ /HO‐1 signaling pathway. Biofactors 45: 598-606.
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Zhu Z, Li R, Lv Y, Zeng W (2019) Melatonin protects rabbit spermatozoa from cryo-damage via decreasing oxidative stress. Cryobiology 88: 1-8.
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Authors and Affiliations

S.A. Akarsu
1
T.C. Acısu
2
İ.H. Güngör
2
A. Çakır Cihangiroğlu
2
R.H. Koca
3
G. Türk
2
M. Sönmez
2
S. Gür
2
F. Fırat
2
H.E. Esmer Duruel
4

  1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Atatürk University, 25240 Yakutiye /Erzurum, Turkey
  2. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey
  3. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bingöl University, Selahaddin-i Eyyübi Neighbourhood, University Street No: 1, 12000, Bingöl, Turkey
  4. Elbistan Vocational School, Kahramanmaraş İstiklal University, Şehit Astsubay Ömer Halisdemir Street No:5, Doğan Neighbourhooh,46340, Elbistan, Kahramanmaraş, Turkey
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Abstract

The purpose of this study was to measure circulating TSH, T 4 and fT 4 concentrations in dogs submitted to a clinical visit for general symptoms (weight gain, polyuria and polydipsia, changes in hair coat). Twenty-eight dogs, 14 cross-breed and 14 purebreds (Golden Retriever, Labrador, Doberman), of both sexes (14 males and 14 females), aged 8 to 14 years, were assessed. No significant differences of circulating TSH, T 4, fT 4 concentrations between the baseline and after therapeutic treatment nor between intact and neutered females were observed. Compared to baseline values, intact males showed higher TSH concentrations (p0.01), and castrated males lower TSH concentrations (p0.01) after therapeutic treatment. Compared to intact males, castrated males showed baseline TSH concentrations higher (p0.01), but lower (p0.01) after therapeutic treatment. No significant differences of T 4 and fT 4 concentrations between baseline conditions and after therapeutic treatment, nor between intact and castrated males, were observed. The experimental sample considered in this study falls within that casuistry involving elevated TSH concentrations but low serum T 4 and fT 4 concentrations or close to the minimum physiological cut-off, in which the common clinical signs suggestive of hypothyroidism was, essentially, overweight and neglected appearance of the hair.
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Authors and Affiliations

O. Bucalo
1
K. Satué
2
P. Medica
3
C. Cravana
3
E. Fazio
3

  1. Pomezia Veterinary Hospital, Via dei Castelli romani 54, 00071 Roma, Italy
  2. Department of Animal Medicine and Surgery, CEU-Cardenal Herrera University, Tirant lo Blanc, 7, Alfara del Patriarca, 46115 Valencia, Spain
  3. Department of Veterinary Sciences, Veterinary Physiology Unit, Messina University, Polo Universitario Annunziata, Viale Palatucci 13, 98168 Messina, Italy
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Abstract

This experimental study aimed to investigate some hematological and immunological changes as a result of Aeromanas hydrophila infection in Siberian sturgeon ( Acipenser baerii Brandt, 1869). Their feeds were supplemented with β-1,3/1,6 glucan at different ratios, 250 mg/kg (βG 250); 500 mg/kg (βG 500) and 750 mg/kg (βG 750). To create an experimental infection, 4×106 cfu/ml Aeromonas hydrophila inoculum was intraperitoneally injected to fish. 0.1 ml intraperitoneal bacteria injection was given to the fish in 12 of 15 tanks, each consisting of 10 fish (the fish in the control group were not given bacteria). Considering the βG 500 and βG 750 group fish as positive (C+) and negative control (C−) groups in terms of hematological parameters, it was found that RBC (erythrocyte) and Hb (hemoglobin) values, as well as RBC indices (MCV, MCHC, MCH) significantly increased. The immunological parameters, including WBC (leukocyte), leukocyte cell percentages (lymphocyte, monocyte, neutrophil, eosinophil), as well as cytokines, including IL-1β, IL-6, IL-8, and TNF-α values showed similar increases in the βG 500 and βG 750 groups. It was found that the addition of 500 and 750 mg/kg doses of β-1,3/1,6 glucan to the feed stimulated non-specific immunity of fish against bacterial agents and/or septicemic diseases and beta glucan at this dosage range was determined to be ideal for fish health and that it may be a herbal immunostimulant that can be an alternative to many medicaments.
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Authors and Affiliations

S. Duman
1
A. Şahan
2

  1. Cukurova University, Imamoglu Vocational School, Department of Aquaculture, 01700, İmamoglu, Adana, Turkey
  2. Cukurova University, Fisheries Faculty, Department of Aquaculture, 01330, Balcalı, Adana, Turkey
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Abstract

African swine fever virus (ASFV) causes feverous and hemorrhagic disease of domestic pigs and European wild boars with high mortality, yet no commercial vaccine is currently available. Several ASFV strains with natural deletion or gene-targeted knockout of multiple MGF360 and MGF505 genes are attenuated in vitro and in vivo, and can offer full protection against homologous challenge. However, the mechanisms underlying the protection are not fully understood. This study aims to investigate the effects of MGF360-12L of ASFV-SY18 on the cGAS-STING signaling pathway and explore the potential mechanisms. We identified that ASFV-SY18 MGF360-12L could inhibit cGAS-STING, TBK1, or IRF3-5D-stimulated IFN-β expression and ISRE activation. Specifically, MGF360-12L inhibits both the activation of PRD(III-I) in a dose-dependent manner, and suppresses the exogenous expression of TBK1 and IRF3-5D. MGF360-12L could block NF-κB activation induced by overexpression of cGAS-STING, TBK1, IKKβ. Downstream of the IFN-β signaling, MGF360-12L blocks the ISRE promoter activation by reducing total protein level of IRF9. Moreover, MGF360-12L protein can inhibit IFN-β-mediated antiviral effects. In conclusion, our findings suggest that MGF360-12L is a multifunctional immune-evasion protein that inhibits both the expression and effect of IFN-β, which could partially explain the attenuation of relevant gene-deleted ASFV strains, and shed light on the development of efficient ASFV live attenuated vaccines in the future.
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Authors and Affiliations

Q. Chen
1
X.X. Wang
2
S.W. Jiang
1
X.T. Gao
3
S.Y. Huang
1
Y. Liang
1
H. Jia
2
H.F. Zhu
2

  1. Key Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resource Environment, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, 102206 Beijing, China
  2. Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, 100193 Beijing, China
  3. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, 100081 Beijing, China
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Abstract

Probiotics and prebiotics are viable bacteria with beneficial effects on the host and components that selectively act on the beneficial commensal bacteria, respectively. The combined use of probiotics and prebiotics is termed synbiotics. Probiotic intake improves dysbiosis in the intestinal microbiota and can positively affect canine atopic dermatitis (CAD). However, clinical studies on improvements in CAD using synbiotics remain limited.
In this study, 15 dogs with CAD who received prednisolone, a synthetic glucocorticoid (GC) used in the treatment of CAD, for more than 90 days were continuously treated with Lactobacillus paracasei M-1 from fermented food as a probiotic, and trisaccharide kestose as a prebiotic, for 90 days to determine their synbiotic effects on CAD. The CAD symptoms were evaluated using the canine atopic dermatitis lesion index (CADLI) and pruritus visual analog scores (PVAS) at 30, 60 and 90 days after synbiotic administration. The total prednisolone use for 90 days pre- and post-administration was also evaluated.
Synbiotic administration significantly reduced the CADLI (pre: median, 28.0 [22.0-32.0]; 30 days: median, 20.0 [20.0−28.0]; 60 days: median, 20.0 [10.0−21.0]; 90 days: median, 12.0 [10.0-19.0]) and PVAS (pre: median, 6.0 [5.0-7.0]; 30 days: median, 3.0 [3.0-3.5]; 60 days: median, 3.0 [3.0-3.5]; 90 days: median, 2.0 [2.0-3.5]) scores, and reduced the total prednisone use over 90 days (pre: 112.0 [25-450] mg; post: 80.0 [18.-300.0] mg; p<0.001) in the 15 dogs. Thus, the synbiotic activity of L. paracasei M-1 and trisaccharide kestose can improve CAD.
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Authors and Affiliations

K. Kawano
1 2
K. Iyori
3
N. Kondo
2 4 5
S. Yamakawa
2 4 5
T. Fujii
6
K. Funasaka
2
Y. Hirooka
2 6
T. Tochio
6

  1. Tokyo Animal Allergy Center, Adachi-ku, Tokyo 123-0842, Japan
  2. Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Aichi 470-1192, Japan
  3. Vet Derm Tokyo, Dermatological and Laboratory Service for Animals, Fujisawa, Kanagawa 252-0823, Japan
  4. Research and Development Division, Itochu Sugar Co., Ltd., Hekinan, Aichi 447-8506, Japan
  5. WELLNEO SUGAR Co., Ltd., Chuo-ku, Tokyo 103-8536, Japan
  6. Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Toyoake, Aichi 470-1192, Japan
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Abstract

Health status of Polish goat population in regard to the viral diseases remained mostly unknown. In order to determine serological status of Polish goats for selected emerging ruminant viruses, 365 serum samples collected between 2017 and 2019 in 36 districts within 10 of Polish provinces, were tested. No antibodies specific to Peste de Petite Ruminants Virus (PPRSV) and capripoxviruses (CaPV) were found in any of the tested animals. Only single individual (0.27%) was seropositive to Blutongue Virus (BTV). Antibodies directed to Schmallenberg Virus (SBV) were detected in 46 goats which represented 12.6% of the tested population. No association between seropositivity to SBV and year of sampling, province of origin, gender and age was found. In conclusion, among studied viral pathogens, currently only SBV seemed to be important for epidemiological status of Polish goats.
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Authors and Affiliations

M. Larska
1
W. Socha
1
J. Rola
1

  1. Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland
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Abstract

Antibiotic resistance has become a global public health concern in the last few years. Given the widespread rate of recurrence, increasing attention is being turned toward environmental pathways that potentially contribute to antibiotic resistance genes (ARGs) dissemination outside the clinical realm. In this study, a metagenome analysis of intestinal virus-like particle fraction (VLPs) from a wild coyote ( Canis latrans) revealed for the first time, multiple ARGs, such as B-lactamases and multidrug efflux pumps. Description of ARGs presence in natural environments is critical to understand the emergence of resistant strains.
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Subirats J, Sánchez-Melsió A, Borrego CM, Balcázar JL, Simonet P (2016) Metagenomic analysis reveals that bacteriophages are reservoirs of antibiotic resistance genes. Int J Antimicrob Agents 48: 163-167.
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Tian R-M, Cai L, Zhang W-P, Cao H-L, Qian P-Y (2015) Rare events of intragenus and intraspecies horizontal transfer of the 16S rRNA gene. Genome Biol Evol 7: 2310-2320.
Zolfo M, Pinto F, Asnicar F, Manghi P, Tett A, Bushman F, Segata N (2019) Detecting contamination in viromes using ViromeQC. Nat Biotechnol 37: 1408-1412.
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Authors and Affiliations

J.J. López-Islas
1
ET. Méndez-Olvera
2
T. Reyes C
2
D. Martínez-Gómez
2

  1. Doctorado en Ciencias Agropecuarias, Autonomous Metropolitan University (UAM), Calzada del Hueso 1100, Villa Quietud, C.P. 04960, Coyoacán, México City, México
  2. Department of Agricultural and Animal Production, Autonomous Metropolitan University, campus Xochimilco (UAM-X), Calzada del Hueso 1100,Villa Quietud, C.P. 04960, Coyoacán, México City, México
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Abstract

In summer 2017 numerous dead round gobies ( Neogobius melanostomus) and individuals covered with white coating were observed in the Puck Bay. The aim of our research was to determine the microbiological composition of the round goby from the Puck Bay, focusing on the presence of pathogens. Bacteria were identified by biochemical methods and, by sequencing of 16S rRNA. The dominant bacterial species were Shewanella baltica, Pseudomonas spp. and Aeromonas spp. - opportunistic pathogens, commonly present in many fish species, which may become harmful for the organism in unfavorable conditions. It was the first trial to determine the composition of the bacterial flora of N. melanostomus from that area.
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Dybowski D, Jakacki J, Janecki M, Nowicki A, Rak D, Dzierzbicka-Glowacka L (2019) High-Resolution Ecosystem Model of the Puck Bay (Southern Baltic Sea) -Hydrodynamic Component Evaluation. Water 11: 2057.
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Authors and Affiliations

K. Nadolna-Ałtyn
1
A. Pękala-Safińska
2
P. Balazy
3
G. Siedlewicz
3
E. Paździor
4
J. Pawlak
1
I. Psuty
1

  1. National Marine Fisheries Research Institute, Kołłątaja 1, 81-332 Gdynia, Poland
  2. Department of Preclinic Sciences and Infectious Diseases, Poznan University of Life Sciences in Poznan, Wołyńska 35, 60-637 Poznań, Poland
  3. Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland
  4. Department of Fish Diseases, National Veterinary Research Institute, Aleja Partyzantów 57, 24-100 Puławy, Poland
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Abstract

Bovine tuberculosis (BTB) is a dangerous zoonosis which presents a serious problem for endangered species such as European bison ( Bison bonasus). Little is known about the influence of parasitic co-infections on the course and diagnosis of tuberculosis in animals. The best known co-infection in cattle is Fasciola hepatica and Mycobacterium bovis. The aim of this study was to review the most recent literature regarding tuberculosis and parasite co-infection in ungulates and relate the results to European bison. Our findings indicate that any comprehensive diagnosis of BTB should include parasitological monitoring, and the possible impact of such invasions on cellular response-based tuberculosis tests should be taken into account. The diagnosis of BTB is complex, as is its pathogenesis, and parasitic infestations can have a significant impact on both. This should be taken into account during further research and monitoring of tuberculosis in European bison.
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Authors and Affiliations

M. Gałązka
1 2
A. Didkowska
1
K. Anusz
1
A. Pyziel-Serafin
1

  1. Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland
  2. Municipal Zoological Garden in Warsaw, Ratuszowa 1/3 03-461, Warsaw, Poland
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Abstract

Despite over 40 years of research on the human immunodeficiency virus type 1 (HIV-1) vaccine, we still lack a considerable progress. Equine infectious anemia virus (EIAV) is a lentivirus in the Retroviridae family, akin to HIV-1 in genome structure and antigenicity. EIA is an important infectious disease in equids, characterized by anemia, persistent infection, and repeated fevers. The EIAV attenuated vaccine in China is the only lentiviral vaccine used on a large scale. Elucidating the mechanism of waning and induction of protective immunity from this attenuated vaccine strain will provide a critical theoretical basis and reference point for vaccine research, particularly in the development of lentivirus vaccines, with far-reaching scientific value and social significance. In this paper, we summarize the information related to EIAV integration site selection, particularly for the Chinese EIAV attenuated vaccine strains on the equine genome. This may improve our mechanistic understanding of EIAV virulence reduction at the host genome level. The obtained data may help elucidate the biological characteristics of EIAV, particularly the Chinese attenuated EIAV vaccine strain, and provide valuable information regarding retroviral infections, particularly lentiviral infection and associated therapeutic vectors.
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Authors and Affiliations

Y.-Y. Yu
1
M.-S. Xu
2
H. Liang
1
H.-Y. Wang
1
C.-Q. Yu
3
Q. Liu
1

  1. Nanchong Key Laboratory of Disease Prevention, Control and Detection in Livestock and Poultry, Nanchong Vocational and Technical College, Nanchong 637131, China
  2. Chongqing Three Gorges Vocational College, Wanzhou 404155, China
  3. School of Advanced Agricultural Sciences, Yibin Vocational Technical College, Yibin 644003, China

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