Disturbance rejection performance optimization with constraints on robustness for a multivariable process is commonly encountered in industrial control applications. This paper presents the tuning of a multi-loop Proportional Integral (PI) controller method to enhance the performance of load disturbance rejection using evolutionary optimization. The proposed design methodology is formulated to minimize the load disturbance rejection response and the input control energy under the constraints of robust stability. The minimum singular value of multiplicative uncertainty is considered a multi-loop system robust stability indicator. Optimization is performed to achieve the same, or higher level than the most-explored Direct Synthesis (DS) based multi-loop PI controller, which is derived from a conventional criterion. Simulation analysis clearly proved that the proposed multi-loop PI controller tuning method gives better disturbance rejection, and either, the same or a higher level of robust stability when compared to the DS-based multi-loop PI controller.