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Abstract

Methane is an atmospheric trace gas, which is estimated to contribute about 20% to global warming. Coal mining used to be regarded as attributing considerably to the anthropogenic emissions of that potent greenhouse gas. Recently discovered methanotrophic abilities of coalbed rocks brought a new argument to the discussion about the environmental impact of the mining industry. In the present work, we determined the methanotrophic activity and maximum capacity (Vmaxl of methane oxidation originating from rocks surrounding seam 385/2 of the "Bogdanka" coal mine. Methane oxidation rates ranged from 0.23lμM CH4 g "day ·1 in the rock from the middle of the seam to 0.619 μM CH4 g "day ·1 in the bottom rock (4.4 m depth). Methanotrophic activity and Ymax increased with the distance to the coal body and with decreasing TOC content. Initial and terminal redox conditions (Eh>320 mV, pH 7.60-8.62) confirmed the oxic character of the methane oxidation process.
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Authors and Affiliations

Zofia Stępniewska
Anna Pytlak
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Abstract

An enzyme-assisted modified steam distillation process was adopted to extract turmeric oil from Curcuma longa L. rhizomes. The diastase, xylose, cellulase, pectinase, and lipase enzymes were used for the pre-treatment of fresh turmeric rhizome to obtain a higher yield by rapturing biological cells. The quantitative and qualitative analysis of turmeric oil was performed by GC–MS. The various influencing parameters for the extraction of turmeric oil such as an enzyme, incubation/pre-treatment time, distillation time have been studied in the presentwork. The obtained turmeric oil by enzymatic pretreatment process is richer in bioactive/medicinal components than in the other traditional methods. The maximum yield was obtained with cellulase enzyme, which is 25–27% higher than the yield obtained by the traditional hydro distillation process. The detailed qualitative and quantitative analyses are also presented. The present method can be considered energy-efficient, effective, economical, and eco-friendly.
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Authors and Affiliations

Avinash Chandra
1
Heena Rekhi
2
Dharmender
3
A.K. Gautam
4
R.K. Arya
5

  1. Department of Chemical Engineering, Thapar Institute of Engineering and Technology, Patiala-147004, India
  2. Department of Chemistry, Khalsa College, Patiala, Punjab-147001, India
  3. School of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology, Patiala-147004, India
  4. Department of Chemical Engineering, National Institute of Technology, Hamirpur-177005, India
  5. Department of Chemical Engineering, Dr. B. R. Ambedkar National Institute of Technology, Jalandhar-144011, India
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Abstract

Infectious keratoconjunctivitis is an infectious disease that negatively affects animal welfare causing systemic or local clinical signs in small ruminants and causes significant economic losses in herds. It is important to determine the etiologic agent causing the infection in the development of the protection and control strategies against the disease. The aim of this study was to determine the presence of infectious keratoconjunctivitis cases in small ruminants raised in Siirt province in Türkiye. Infectious keratoconjunctivitis was graded according to the symptoms determined by clinical examination. The presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis was investigated by PCR in swab samples obtained from the animals with keratoconjunctivitis. Infectious keratoconjunctivitis was detected in 263 (19.86%) of 1324 animals examined in the study. Of the animals with infectious keratoconjunctivitis, 163 (61.97%) were sheep and 100 (38.02%) were goats. The detection rate of infectious keratoconjunctivitis was higher in sheep than goats. In 56 (21.29%), 109 (41.44%), 67 (25.47%), and 31 (11.78%) of the cases, findings of stage 1, 2, 3, and 4 infectious keratoconjunctivitis were detected, respectively. Of the eye swab samples taken from 263 animals with infectious keratoconjunctivitis, 5 (1.90%) were positive for Mycoplasma spp. and 6 (2.28%) were positive for M. ovis. It was determined that the distribution of the bacterial agents varied according to the stage of infectious keratoconjunctivitis. No statistically significant correlation was found in the distribution of bacterial agents among identified samples according to species, sex, age, and infectious keratoconjunctivitis stage of the animals. It was thought that the data obtained in the study would contribute to the studies for protection and control by determining the incidence and aetiology of infectious keratoconjunctivitis cases observed in small ruminants.
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Bibliography

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Authors and Affiliations

A. Gulaydin
1
O. Gulaydin
2
M.B. Akgul
1
N. Sindak
1
O. Yildirim
1

  1. Siirt University, Faculty of Veterinary Medicine, Department of Surgery, Siirt/Türkiye
  2. Siirt University, Faculty of Veterinary Medicine, Department of Microbiology, Siirt/Türkiye
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Abstract

Numbers of saprophytic bacteria were determined by the plate count in samples obtained at 45 oceanographic stations, from six standard depths between 10 and 150 m. Depending on the sampling place, the numbers of bacteria fluctuated between 0.8 x 102 to 4.3 x l 04 x 1-1 and 1.2 x l07 to 1.3 x 108, in a water column under 1 m2 sea surface. Most of saprophytic bacteria were observed at stations located south and south-east of the King George Island, and also north and north-west of the Anvers Island. Fewer numbers were found in areas of large krill swarms in the Bransfield Strait, between 58°30' and 62°30' W, and in the north-western part of the research area, far away from the South Shetland Islands.

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Authors and Affiliations

Marek K. Zdanowski
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Bibliography

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Authors and Affiliations

Artur Kosecki
1
ORCID: ORCID

  1. Uniwersytet Szczeciński, Instytut Filozofii i Kognitywistyki, ul. Krakowska71/79, 71-017 Szczecin

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