This study aims at the immobilization and characterization of thermoalkalophilic lipases produced recombinantly from Bacillus thermocatenulatus BTL2 and Bacillus pumilus MBB03. For this purpose, immobilization of the produced enzymes in calcium-alginate@gelatin (Ca–Alg@gelatin) hydrogel beads, immobilization optimization and characterization measurements of the immobilized-enzyme hydrogels were conducted. Optimum temperature and pH values were determined for B. thermocatenulatus and B. pumilus MBB03 immobilized-enzyme hydrogels (60–70 °C, 55 °C and pH 9.5, pH 8.5). Thermal stability was determined between 65 °C and 60 °C of B. thermocatenulatus and B. pumilus MBB03 immobilized enzymes, respectively. The pH stability was determined between pH 7.0–11.0 at +4°C and pH 8.0–10.0 at +4 °C, respectively.
In conclusion, the entrapment technique provided controlled production of small diameter hydrogel beads (~ 0:19 and ~ 0:29) with negligible loss of enzyme. These beads retained high lipase activity at high pH and temperature. The activity of Ca–Alg@gelatin-immobilized lipase remained relatively stable for up to three cycles and then markedly decreased. With this enzyme immobilization, it may have a potential for use in esterification and transesterification reactions carried out in organic solvent environments. We can conclude that it is one of the most promising techniques for highly efficient and economically competent biotechnological processes in the field of biotransformation, diagnostics, pharmaceutical, food and detergent industries.

Understanding the Cenozoic tectonic evolution of grabens rich in lignite is important in the context of the accumulation of ~40–650 m of peat, as well as the exploitation of later formed lignite seams with a thickness of ~20–250 m. Six such areas were selected for a detailed palaeotectonic analysis: the Gostyń, Szamotuły, Legnica, Zittau, Lubstów, and Kleszczów grabens. During the analysis, borehole data were used, taking into account the compaction of peat at the transition to lignite, in order to reconstruct the magnitude of the total subsidence. This made it possible to distinguish between regional (covering areas also outside the grabens) and local (occurring only in the grabens) tectonic movements, and among the latter, tectonic and compactional subsidence. The hypothetical palaeosurface of the mires was reconstructed based on the lignite decompaction. As a result, it was possible to determine whether the examined peat/lignite seams underwent post-depositional uplift and/or subsidence. Between one (Gostyń Graben) and four (Zittau Basin and Kleszczów Graben) stages of tectonic subsidence were distinguished in the studied lignite-bearing areas. In the case of the Zittau Basin, as well as the Lubstów and Kleszczów grabens, post-depositional stages of tectonic uplift were also indicated. Like the boundaries of lithostratigraphic units, the successive stages of the Cenozoic tectonic development of the examined grabens are diachronic.