References:

Abdelmonem R, Younis MK, Hassan DH, El-Sayed Ahmed M, Hassanein E, El-Batouty K, Elfaham A (2019) Formulation and characterization of chlorhexidine HCl nano-emulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies. Int J Nanomedicine 14: 4697-4708.
Badruddoza AZ, Gupta A, Myerson AS, Trout BL, Doyle PS (2018) Low energy nanoemulsions as templates for the formulation of hydrophobic drugs. Adv Ther 1: 1700020.
Boyce JM (2016) Modern technologies for improving cleaning and disinfection of environmental surfaces in hospitals. Antimicrob Resist Infect Control 5: 10.
Chen LH, Cheng LC, Doyle PS (2020) Nanoemulsion-loaded capsules for controlled delivery of lipophilic active ingredients. Adv Sci 7: 2001677.
Chepurnov AA, Bakulina LF, Dadaeva AA, Ustinova EN, Chepurnova TS, Baker JR Jr (2003) Inactivation of Ebola virus with a surfactant nanoemulsion. Acta Trop 87: 315-320.
Dancer SJ (2014) Controlling hospital-acquired infection: focus on the role of the environment and new technologies for decontamination. Clin Microbiol Rev 27: 665-690.
Eissa M, Ashour ED, Mansy MS (2012) Neutralizer evaluation study of some microbial isolates against two strong disinfectants with and without the presence of synthetic detergent. World Appl Sci J 20: 823-831.
Hamouda T, Hayes MM, Cao Z, Tonda R., Johnson K, Wright DC, Brisker J, Baker JR Jr (1999) A novel surfactant nanoemulsion with broad-spectrum spori-cidal activity against Bacillus species. J Infect Dis 180: 1939-1949.
Hamouda T, Myc A, Donovan B, Shih AY, Reuter JD, Baker JR Jr (2001) A novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial activity against bacteria, enveloped viruses and fungi. Microbiol Res 156: 1-7.
Han JH, Sullivan N, Leas BF, Pegues DA, Kaczmarek JL, Umscheid CA (2015) Cleaning hospital room surfaces to prevent health care-associated infections: a technical brief. Ann Intern Med 163: 598-607.
Hashemnejad SM, Badruddoza AZ, Zarket B, Ricardo Castaneda C, Doyle PS (2019) Thermoresponsive nanoemulsion-based gel synthesized through a low-energy process. Nat Commun 10: 2749.
Hidber T, Pauli U, Steiner A, Kuhnert P (2020) In vitro and ex vivo testing of alternative disinfectants to currently used more harmful substances in footbaths against Dichelobacter nodosus. PLoS One 15: e0229066.
Horstmann Risso N, Ottonelli Stopiglia CD, Oliveira MT, Haas SE, Ramos Maciel T, Reginatto Lazzari N, Kelmer EL, Pinto Vilela JA, Beckmann DV (2020) Chlorhexidine nanoemulsion: a new antiseptic formulation. Int J Nanomedicine 15: 6935-6944.
Hu GZ, Qiu YS (2010) Medicines commonly used in poultry and their rational use. Henan Science and Technology Press, Zhengzhou, p 27.
Matsubara T, Maki S, Toshimori Y (2021) The effectiveness of a nonalcoholic disinfectant containing metal ions, with broad antimicrobial activity. Sci Rep 11: 1072.
Ministry of Health of the People’s Republic of China (2008) Technical standard for disinfection. Ministry of Health of the People’s Republic of China, Beijing, pp 21-52.
Mu SY, Liu DY, Bai YZ, Yang WY, Shi YL, Li S, Ning MX, Yang XF (2016) Disinfection efficacy of chlorocresol nanoemulsion disin-fectant. Chin J Vet Med 52: 35-37.
Ramalingam K, Frohlich NC, Lee VA (2013) Effect of nanoemulsion on dental unit waterline biofilm. J Dent Sci 8: 333-336.
Roedel A, Vincze S, Projahn M, Roesler U, Robé C, Hammerl JA, Noll M, Al Dahouk S, Dieckmann R (2021) Genetic but no phenotypic associations between biocide tolerance and antibiotic resistance in Escherichia coli from german broiler fattening farms. Microorganisms 9: 651.
Wei QH, Zhang WF, Wang CD, Lu Y, Wang JY, Zhang M (2004) Experimental observation on properties of a compound germicidal nanoemulsion. Chin J Dis 21: 1-4.
Yang XF, Qi YH, Ning HM, Wang QH (2012) Preparation and quality evaluation of enrofloxacin nanoemulsion. J Zhejiang Univ (Agric & Life Sci) 38: 693-699.
Yang XF, Sun YW, Mu SY, Liu DY, Hu JH, Xu YZ, Bai YZ, Shi YL (2016) Evaluation of characterization and disinfection efficacy of chlorocresol nanoemulsion disinfectant. RSC Adv 6: 12730-12736.
Yin M, Zhang DL, Sun YJ, Li XH, Li YY, Xu P, Xue MQ, Jin MY, Yang XF (2020) Fungicidal effect of chlorcresol nanoemulsion disinfectant. J Northwest A&F Univ (Nat Sci Ed) 48: 18-23.

1. Brown J, Poonsuk K, Cheng TY, Rademacher C, Kalkwarf E, Tian L, McKeen LA, Wang C, Gimenez-Lirola L, Baum D, Karriker LA (2023) Comparison of two diagnostic assays for the detection of serum neutralizing antibody to porcine epidemic diarrhea virus. Animals (Basel) 13:757.
2. Hsueh FC, Chang YC, Kao CF, Hsu CW, Chang HW (2020) Intramuscular immunization with chemokine-adjuvanted inactive porcine epidemic diarrhea virus induces substantial protection in pigs. Vaccines (Basel) 8:102.
3. Jin H, Wu Y, Bi S, Xu Y, Shi F, Li X, Ma X, Hu S (2020) Higher immune response induced by vaccination in Houhai acupoint relates to the lymphatic drainage of the injection site. Res Vet Sci 130: 230-236.
4. Jung K, Saif LJ, Wang Q (2020) Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and pre-vention and control. Virus Res 286: 198045.
5. Krishna VD, Kim Y, Yang M, Vannucci F, Molitor T, Torremorell M, Cheeran MC (2020) Immune responses to porcine epidemic diar-rhea virus (PEDV) in swine and protection against subsequent infection. PLoS One 15: e0231723.
6. Langel SN, Paim FC, Alhamo MA, Buckley A, Van Geelen A, Lager KM, Vlasova AN, Saif LJ (2019) Stage of gestation at porcine epidemic diarrhea virus infection of pregnant swine impacts maternal immunity and lactogenic immune protection of neonatal suckling piglets. Front Immunol 10: 727.
7. Lee C (2015) Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus. Virol J 12: 193.
8. Lv C, Xiao Y, Li X, Tian K (2016) Porcine epidemic diarrhea virus: current insights. Virus Adapt Treat 8: 1-12.
9. Shibata I, Tsuda T, Mori M, Ono M, Sueyoshi M, Uruno K (2000) Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages. Vet Microbiol 72: 173-182.
10. Sun D, Wang X, Wei S, Chen J, Feng L (2016) Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review. J Vet Med Sci 78: 355-363.
11. Xu W, Hu S (2021) Administration of infectious bursal disease vaccine in Houhai acupoint promotes robust immune responses in chickens. Res Vet Sci 142: 149-153.

1. Bai Z, Cao Y, Liu W, Li J (2021) The SARS-CoV-2 nucleocapsid protein and its role in viral structure, biological functions, and a poten-tial target for drug or vaccine mitigation. Viruses 13: 1115.
2. Bates TA, Weinstein JB, Farley S, Leier HC, Messer WB, Tafesse FG (2021) Cross-reactivity of SARS-CoV structural protein antibod-ies against SARS-CoV-2. Cell Rep 34: 108737.
3. Chen K, Xiao F, Hu D, Ge W, Tian M, Wang W, Pan P, Wu K, Wu J (2020) SARS-CoV-2 nucleocapsid protein interacts with RIG-I and represses RIG-mediated IFN-β production. Viruses 13: 47.
4. Chen Y, Liu Q, Guo D (2020) Emerging coronaviruses: genome structure, replication, and pathogenesis. J Med Virol 92: 418-423.
5. Clementi N, Ghosh S, De Santis M, Castelli M, Criscuolo E, Zanoni I, Clementi M, Mancini N (2021) Viral respiratory pathogens and lung injury. Clin Microbiol Rev 34: e00103-00120.
6. Guo L, Ren L, Yang S, Xiao M, Chang D, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, Zhang L, Han L, Dang S, Xu Y, Yang QW, Xu SY, Zhu HD, Xu Y,C Jin Q, Sharma L, Wang L, Wang J (2020) Profiling early humoral response to diagnose novel corona-virus disease (COVID-19). Clin Infect Dis 71: 778-785.
7. Gutiérrez-González M, Farías C, Tello S, Pérez-Etcheverry D, Romero A, Zúñiga R, Ribeiro CH, Lorenzo-Ferreiro C, Molina MC (2019) Optimization of culture conditions for the expression of three different insoluble proteins in Escherichia coli. Sci Rep 9: 16850.
8. Han Y, Luo Z, Zhai W, Zheng Y, Liu H, Wang Y, Wu E, Xiong F, Ma Y (2020) Comparison of the clinical manifestations between dif-ferent age groups of patients with overseas imported COVID-19. PLoS One 15: e0243347.
9. Ji T, Liu Z, Wang G, Guo X, Akbar khan S, Lai C, Chen H, Huang S, Xia S, Chen B, Jia H, Chen Y, Zhou Q (2020) Detection of COVID-19: A review of the current literature and future perspectives. Biosens Bioelectron 166: 112455.
10. Jin Q, Yang J, Lu Q, Guo J, Deng R, Wang Y, Wang S, Wang S, Chen W, Zhi Y, Wang L, Yang S, Zhang G (2012) Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2. J Vet Diagn Invest 24: 1151-1157.
11. Jin Y, Yang H, Ji W, Wu W, Chen S, Zhang W, Duan G (2020) Virology, epidemiology, pathogenesis, and control of COVID-19. Vi-ruses 12: 372.
12. Lauer SA, Grantz KH, Bi Q, Jones FK, Zheng Q, Meredith HR, Azman AS, Reich NG, Lessler J (2020) The incubation period of coro-navirus disease 2019 (COVID-19) from publicly reported confirmed cases: estimation and application. Ann Intern Med 172: 577-582.
13. Leung NH (2021) Transmissibility and transmission of respiratory viruses. Nat Rev Microbiol 19: 528-545.
14. Liao M, Yan J, Wang X, Qian H, Wang C, Xu D, Wang B, Yang B, Liu S, Zhou M, Gao Q, Zhou Q, Luo J, Li Z, Liu W (2020) De-velopment and clinical application of a rapid SARS-CoV-2 antibody test strip: A multi-center assessment across China. J Clin Lab Anal 35: e23619.
15. Liu P, Zong Y, Jiang S, Jiao Y, Yu X (2021) Development of a nucleocapsid protein-based ELISA for detection of human IgM and IgG antibodies to SARS-CoV-2. ACS Omega 6: 9667-9671.
16. Lv Y, Ma Y, Si Y, Zhu X, Zhang L, Feng H, Tian D, Liao Y, Liu T, Lu H, Ling Y (2021) Rapid SARS-CoV-2 antigen detection poten-tiates early diagnosis of COVID-19 disease. Biosci Trends 15: 93-99.
17. Mak GC, Cheng PK, Lau SS, Wong KK, Lau CS, Lam ET, Chan RC, Tsang DN (2020) Evaluation of rapid antigen test for detection of SARS-CoV-2 virus. J Clin Virol 129: 104500.
18. Malik YA (2020) Properties of coronavirus and SARS-CoV-2. Malays J Pathol 42: 3-11.
19. Meyer NJ, Gattinoni L, Calfee CS (2021) Acute respiratory distress syndrome. Lancet 398: 622-637.
20. Okba NM, Müller MA, Li W, Wang C, GeurtsvanKessel CH, Corman VM, Lamers MM, Sikkema RS, de Bruin E, Chandler FD, Yazdanpanah Y, Hingrat QL, Descamps D, Houhou-Fidouh N, Reusken CB, Bosch BJ, Drosten C, Koopmans MP, Haagmans BL (2020) Severe acute respiratory syndrome coronavirus 2-specific antibody responses in coronavirus disease patients. Emerg Infect Dis 26: 1478-1488.
21. Rump A, Risti R, Kristal ML, Reut J, Syritski V, Lookene A, Boudinot SR (2021) Dual ELISA using SARS-CoV-2 nucleocapsid pro-tein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation. Biochem Biophys Res Commun 534: 457-460.
22. Singh A, Upadhyay V, Upadhyay AK, Singh SM, Panda AK (2015) Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process. Microb Cell Fact 14: 41.
23. Tian Y, Zhang G, Liu H, Ding P, Jia R, Zhou J, Chen Y, Qi Y, Du J, Liang C, Zhu X, Wang A (2022) Screening and identification of B cell epitope of the nucleocapsid protein in SARS-CoV-2 using the monoclonal antibodies. Appl Microbiol Biotechnol 106: 1151-1164.
24. Vashisht K, Goyal B, Pasupureddy R, Na BK, Shin HJ, Sahu D, De S, Chakraborti S, Pandey KC (2023) Exploring the immunodomi-nant epitopes of SARS-CoV-2 nucleocapsid protein as exposure biomarker. Cureus 15: e34827.
25. Wang Y, Liu X, Tao L, Xu P, Gao X, Li H, Yang Z, Wu W (2017) Expression and immunogenicity of VP40 protein of ZEBOV. Arch Iran Med 20: 246-250.
26. Wang YB, Li YH, Li QM, Xie WT, Guo CL, Guo JQ, Deng RG, Zhang GP (2019) Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals. Pol J Vet Sci 44: 717-723.
27. Zeng W, Liu G, Ma H, Zhao D, Yang Y, Liu M, Mohammed A, Zhao C, Yang Y, Xie J, Ding C, Ma X, Weng J, Gao Y, He H, Jin T (2020) Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochem Biophys Res Commun 527: 618-623.
28. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL (2020) A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579: 270-273.