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Abstract

In the present study, we used next-generation sequencing to investigate the impacts of two commercially available prescription diet regimens on the fecal microbiomes of eleven client-owned healthy pet dogs. We tested an anallergenic diet on 6 dogs and a low-fat diet on 5 dogs. Before starting the study, each dog was fed a different commercial diet over 5 weeks. After collecting pre-diet fecal samples, the anallergenic or low-fat diet was administered for 5 weeks. We then collected fecal samples and compared the pre- and post-diet fecal microbiomes. In the dogs on the anallergenic diet, we found significantly decreased proportions of Bacteroides, Ruminococcaceae, and Fusobacteriaceae, belonging to the phyla Bacteroidetes, Firmicutes, and Fusobacteria, respectively. The proportion of the genus Streptococcus belonging to the phylum Firmicutes was significantly increased upon administering the anallergenic diet. In the dogs on the low-fat diet, although the phyla Actinobacteria and Bacteroidetes tended to increase (p=0.116) and decrease (p=0.147) relative to the pre-diet levels, respectively, there were no significant differences in the proportions of any phylum between the pre- and post-diet fecal microbiomes. The anallergenic diet induced a significantly lower diversity index value than that found in the pre-diet period. Principal coordinate analysis based on unweighted UniFrac distance matrices revealed separation between the pre- and post-diet microbiomes in the dogs on the anallergenic diet. These results suggest that, even in pet dogs kept indoors in different living environments, unification of the diet induces apparent changes in the fecal microbiome.
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Authors and Affiliations

E. Onozawa
1
A. Goto
1
H. Oda
1
S. Seki
1
T. Sako
1
A. Mori
1

  1. School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan, Musashino, Tokyo 180-8602, Japan
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Abstract

This study used ground penetrating radar soundings to examine a tongue-shaped rock glacier (64°04’S 58°25’W) on James Ross Island, Antarctic Peninsula, in January 2005. The rock glacier studied has multiple well-developed transverse ridges and approximately 800 m long from the talus of its head to its frontal slopes and is 300 m wide in the middle. The longitudinal ground penetrating radar profile identified debris bands which dip up-glacier, similar to the thrust structures in the compression zone of a valley glacier. Transverse ground penetrating radar profiles indicated a layered structure which is inclined towards the central part of the rock glacier and which resembles the transverse foliation of a valley glacier. Consequently, the internal structure of the rock glacier is revealed as being similar to the “nested spoons” common in the interior of valley glaciers. We concluded that this rock glacier has been created by the deformation of a glacier ice core and a thick and continuous debris mantle.

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Authors and Affiliations

Kotaro Fukui
Toshio Sone
Jorge A. Strelin
Cesar A. Torielli
Junko Mori
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Abstract

A glacier lake outburst flood occurred on James Ross Island, Antarctic Peninsula region, during the 2004-2005 austral summer season. The source lake was located on the Lachman II ice-cored rock glacier, and formed prior to 1980. The size of the lake has been increasing gradually since the 1990s. The lake basin extended to approximately 220 m in length and 160 m in width by the end of February 2005. We observed that the lake had drained by February 2005, and found a deep gully on the south side of the lake rim. It appears that the lake level rose and water overflowed the lake rim here. James Ross Island contains a large number of debris-covered glaciers, ice-cored moraines, and rock glaciers with glacier lakes which are dammed by these features or which form upon them. As climatic warming has recently been reported for this region, further glacier lake outburst floods seem likely to occur.

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Authors and Affiliations

Toshio Sone
Kotaro Fukui
Jorge A. Strelin
Cesar A. Torielli
Junko Mori
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Abstract

Large-scale stone-banked lobes and terraces are distributed over an area of 1 km2 of gentle slope on Rink Plateau in the northern part of James Ross Island, Antarctic Peninsula region. Topographically, there are two main features: relatively high risers up to 5 m high and distinct frontal ridges. In order to understand the processes responsible for these lobes and terraces, the authors have monitored air and ground temperatures and movement of stones on the surface over the period 1995-2005. In February 2005, the subsurface structures were surveyed by ground penetrating radar and drilling. The ground penetrating radar profiles identified the bedrock surface. The surface morphology of the lobes corresponds closely with that of the bedrock. The relatively high risers of these lobes are presumed to be due to a cessation of frontal advance.

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Authors and Affiliations

Junko Mori
Kotaro Fukui
Toshio Sone
Jorge A. Strelin
Cesar A. Torielli
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Abstract

The effects of feeding, fasting, and re-feeding on the ruminal profile of growing cattle were studied. Ruminal fluid and urine samples were obtained from 12 crossbred steers weighing approximately 300 kg during the following periods: 11 h of normal feeding (postprandial period), 48 consecutive hours of fasting, and followed by 48 h of re-feeding. Fasting promotes changes in the ruminal profile, such as an increase in ruminal pH, reduction in the number of rumen protozoa and bacteria, and decrease in the urinary excretion of allantoin; however, it does not change the urinary uric acid excretion rate. The overall mean ruminal pH was higher during fasting (7.53±0.27) in comparison to those at normal feeding (6.72±0.25) and re-feeding (6.62±0.31) (p<0.05). During re-feeding, the ruminal profile returned to normal, except for the protozoa count, which despite a slight increase only after 48 h of re-feeding, did not recover to baseline values.
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Authors and Affiliations

E.L. Ortolani
1
C.A. Maruta
1
R.A. Barrêto Júnior
2
C.S. Mori
1
A.C. Antonelli
3
M.C.A. Sucupira
1
A.H.H. Minervino
4

  1. Department of Clinical Science, School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo-SP, 05508-270, Brazil
  2. Department of Animal Science, Federal University of Semiarid, 59625-900, Mossoró-RN, Brazil
  3. School of Veterinary Medicine, Federal University of Sao Francisco Valley, 56304-205, Petrolina-PE, Brazil
  4. Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, 68040-255 Santarém-PA, Brazil
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Abstract

The present study aimed to evaluate the efficiency of hypertonic saline solution (HSS) as a novel treatment of acute ruminal lactic acidosis (ARLA) in cattle, focusing on urinary excretion of acids. Twelve cannulated steers were submitted to experimentally induced ARLA by adminis- tering sucrose into the rumen. Twenty hours later, the cattle were randomly divided into two equal groups. The first group was treated with 7.5% HSS (5 mL/kg) over 15 min, and isotonic saline solution (ISS; 20 mL/kg) for the subsequent 165 minutes. The control group was administered ISS instead of HSS. Rumen and urine samples were collected at different times during the experiment from the baseline to 64 h post-induction. The induction caused a medium-to-moderate ruminal acidosis, and a moderate degree of systemic acidosis and dehydration. Steers treated with HSS increased by 50% its glomerular filtration rate (1.61 mL/min) compared to ISS group (1.06 mL/ min; p<0.03). The overall volume of urine excreted by HSS group was higher than that in ISS group (1.62 L vs 0.7 L; p<0.02). This increase in total volume of urine provided by HSS favored a greater excretion of H+ ions in urine, which was 3.39-fold higher in HSS group (64.3*10-7 vs 18.9*10-7 Mol) as well as lactate (241.7 vs 181.8 mMol) and P urinary excretion (3.8 vs 1.1 mMol) that reduced the urine pH (5.3 vs 5.7). Only the HSS group decreased significantly blood total lactic acid concentration (20.3 %) throughout the treatment. A positive relationship was found between the excretion of urinary phosphorus and urinary pH (r2=0.562). The results showed that this novel treatment with HSS enhanced renal excretion of acids and may be recommended as an additional treatment for cattle with lactic acidosis.

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Authors and Affiliations

F.A.M.L. Rodrigues
A.H.H. Minervino
R.A. Barrêto Júnior
L.F. Reis
R.N.F. Ferreira
C.S. Mori
F.L.C. Oliveira
R.S. Sousa

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