The present study was to reflect the use of some bacteria in the treatment and removal of pollutants in three selected wastewater sites, including a vegetable oil plant (viz. Al-Etihad Food Industries), the main wastewater treatment station in the city of Hila, and Al-Hila River water from October 2019 to January 2020. The bacterial isolates identified in these three sites were Klebsiella pneumoniae, Escherichia coli, Enterobacteria cloacae, Pseudomonas aeruginosa, Thalasobacillus devorans, Acinetobacter baumannii, and Bacillus subtilis. The molecular study of the bacterial isolates involved the detection of bacterial genera using the polymerase chain reaction (PCR). The results showed that water had a variable nature, depending on the substances in it. It recorded varying chemical and physical property values, ranging between 6.36 and 7.82 for pH and from 2500 to 7100 mg∙dm–3 for total alkalinity. Additional values were 713–2051 μS∙cm–1 for electrical conductivity (EC), 5.90–9.80 mg∙dm–3 for chemical oxygen demand (COD), and 480–960 mg∙dm–3 for total hardness. The given values were also 0.20–0.65 μg∙dm–3, 0.03-0.23 μg∙dm–3, and 0–107 mg∙dm–3 for nitrite (NO2), phosphate (PO4) oils, respectively.

In this investigation, the surface characteristics of Nickel based superalloy Inconel-625 were evaluated by the electrical discharge machining with used cooking oil-based biodiesel as a dielectric. Nickel-based superalloys find wide applicability in numerous industries due to their specific properties. The Cu electrodes of various densities prepared by atomic diffusion additive manufacturing process were used for machining. A comparison of the performance was made based on average surface roughness. The Design-expert software was used for experimental design and parametric analysis. The outcome demonstrated that bio-dielectric fluid produced improved surface characteristics. The surface roughness was observed to reduce. The surface micrograph obtained from scanning electron microscopy also confirms a better surface finish of bio-dielectric fluid over EDM oil. The surface roughness was shown to be most significantly influenced by the discharge current, with the other parameters having little or no effect. The results showed that for bio-dielectric, the lowest Ra was 0.643 µm, and for EDM oil, the highest value of 0.844 µm. The slightest difference in roughness value for two dielectric fluids was 0.013 µm, and the highest difference was 0.115 µm.

Cosmetic emulsion bases containing extracts from natural plants were produced. The emulsifier was an aqueous solution of self-emulsifying base made from apricot kernel oil and soy lecithin, while the oil phase was based on coconut, almond or grape seed oils. In addition, mixtures enriched with vegetable glycerine were produced. It was found that for the emulsions with almond oil as the concentration of the oil phase increased, the value of the average Sauter diameter increased. In comparison, results for emulsions with coconut oil and emulsions with grapeseed oil did not give such a clear relationship. It was also shown that for stable emulsions, the self-emulsifying base of apricot kernel oil performed much better than soy lecithin. The addition of vegetable glycerine to the mixture resulted in a reduction of the average droplet diameter. Produced emulsions were also visually observed for 60 days to assess their stability and possible aging processes. In order to exclude the formation of microorganisms, periodic density control and microscopic examinations were carried out. The presence of microorganisms 30 in the analysed emulsion was evaluated using microscopic and culture techniques. No tarnish waso bserved on the surface of the samples, indicating the formation of mould, which can lead to poisoning and the development of allergies, respiratory diseases, liver diseases, ulcers, or bleeding in the intestines.