A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR

Journal title

Journal of Plant Protection Research




vol. 56


No 1


Divisions of PAS

Nauki Biologiczne i Rolnicze


Committee of Plant Protection PAS ; Institute of Plant Protection – National Research Institute




DOI: 10.1515/jppr-2016-0014 ; ISSN 1427-4345 ; eISSN 1899-007X


Journal of Plant Protection Research; 2016; vol. 56; No 1


Ippolito (2004), Realtime detection of Phytophthora nicotianae and P citrophthora in citrus roots and soil, European Journal of Plant Pathology, 110, 833, ; Khanuja (1999), Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils, Plant Molecular Biology Reporter, 17, 74, ; Wiejacha (2002), Optimization of DNA isolation procedure as the first step in identification of Phytophthora spp Bulletin of the Polish Academy of Sciences Biological www qiagen com Accessed, Sciences, 50, 165. ; Bashalkhanov (2008), Protocol A high - throughput DNA extraction system suitable for conifers, Plant Methods, 4. ; Aljanabi (1997), Universal and rapid salt - extraction of high quality genomic DNA for PCR - based techniques, Nucleic Acids Research, 25, 4692, ; Michiels (2003), den Ende Extraction of high - quality genomic DNA from latex - containing plants, Analytical Biochemistry, 315. ; Hu (2003), Target region amplification polymorphism : A novel marker technique for plant genotyping, Plant Molecular Biology Reporter, 21, 289, ; Sahu (2012), DNA extraction protocol for plants with high levels of secondary metabolites and polysaccharides without using liquid nitrogen and phenol International Scholarly Research Network , International Scholarly Research Notices ( ISRN, Molecular Biology, ; Demeke (2010), Influence of DNA extraction methods , PCR inhibitors and quantification methods on realtime PCR assay of biotechnology - derived traits, Analytical and Bioanalytical Chemistry, 396. ; Weishing (1994), DNA isolation and purification In : DNA Fingerprinting in Plants and Fungi eds CRC Press Florida, USA, 44. ; Böhm (1999), Real - time quantitative PCR : DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants, Journal of Phytopathology, 7, 147. ; OEPP (2006), Diagnostic protocol for Phytophthora ramorum OEPP, EPPO Bulletin, 36, 145. ; Wiejacha (2001), Molecular markers in detection of distant hybrids in Lilium, Acta Horticulturae, 546. ; Porebski (1997), Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components, Plant Molecular Biology Reporter, 15, 8, ; Doyle (1987), A rapid DNA isolation procedure for small quantities of fresh leaf tissues, Phytochemical Bulletin, 19, 11. ; Martin (2004), Molecular detection of Phytophthora ramorum , the causal agent of sudden oak death in California , and two additional species commonly recovered from diseased plant material, Phytopathology, 94, 621, ; Caprar (2014), Optimization of DNA isolation from four species of Rhododendron from Europe of Horticulture Forestry and Biotechnology, Journal, 18, 117. ; Erwin (1996), Phytophthora Diseases Worldwide APS Press St Minnesota, USA, 562. ; Csaikl (1998), Comparative analysis of different DNA extraction protocols fast universal maxi - preparation of high quality plant DNA for genetic evaluation and phylogenetic studies, Plant Molecular Biology Reporter, 16, 69, ; Themann (2002), Observation of Phytophthora in water recirculation systems in commercial hardy ornamental nursery stock, European Journal of Plant Pathology, 108, 337, ; Drábková (2002), Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of Juncaceae, Plant Molecular Biology Reporter, 20, 161, ; Terry (2002), Detection of genetically modified crops and their derivatives : critical steps in sample preparation and extraction, Journal of AOAC International, 85, 768.