Title

Effect of Tris-Citric-Fructose-Asolectin extender on mitochondrial activity and intracellular reactive oxygen species in cryopreserved dog semen

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2026

Volume

Vol. 29

Issue

No. 1

Authors

Pulkowska-Bluj, O. ; Trzcińska, M. ; Stefanowicz, P.

Affiliation

Pulkowska-Bluj, O. : Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland ; Trzcińska, M. : Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland ; Stefanowicz, P. : RETINA Veterinary Clinic, Szyllinga 3, 30-433 Kraków, Poland

Keywords

asolectin ; dog ; semen cryopreservation ; sperm analysis

Divisions of PAS

Nauki Biologiczne i Rolnicze

Coverage

39–47

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliography

• Agnihorti SK, Agrawal AK, Hakim BA, Vishwakarma AL, Narender T, Sachan R, Sachdev M (2016) Mitochondrial membrane potential (MMP) regulates sperm motility. In Vitro Cell Dev Biol Anim 52: 953-960.
• Asosingh K (2020) Flow cytometry of male reproductive potential. Cytometry A 97: 1209-1210.
• Axnér E, Lagerson E (2016) Cryopreservation of dog semen in a Tris extender with 1% or 2% soya bean lecithin as a replacement of egg yolk. Reprod Domest Anim 51: 262-268.
• Beccaglia M, Anastasi P, Luvoni GC (2009) Freezing of canine semen in an animal-free protein extender. Vet Res Commun 33: 77-80.
• Brito LF, da Silva MC, Kolster KA (2025) Standardisation of Dog Sperm Morphology Classification. Reprod Domest Anim 60: e70024.
• Dalmazzo A, Losano JD, Rocha CC, Tsunoda RH, Angrimani DS, Mendes CM, Assumpção ME, Nichi M Barnabe VH (2018) Effects of Soy Lecithin Extender on Dog Sperm Cryopreservation. Anim Biotechnol 29: 174-182.
• Ďuračka M, Benko F, Kačániová M, Tvrdá E (2024) Comparative study: Efficacy of egg-yolk vs soy lecithin-based diluent in preservation of chilled bovine semen – Bacteriology and sperm quality. Czech J Anim Sci 69: 400-409.
• Evenson DP, Thompson L, Jost L (1994) Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility. Theriogenology 41: 637-651.
• Farstad W (2009) Cryopreservation of canine semen – new challenges. Reprod Domest Anim 44: 336-341.
• Foutouhi A, Meyers S (2022) Comparative oxidative metabolism in mammalian sperm. Anim Reprod Sci 247: 107095.
• Gonzales A, Arando A, Acosta A, Alcalà CJ, Arrebola FA, Perez-Martin CC (2019) Frozen dog spermatozoa are negatively affected during storage at -80, -21 and -8 ºC. Anim Reprod Sci 210: 106197.
• Hermansson U, Johannisson A, Axnér E (2021) Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender. Vet Med Sci 7: 812-819.
• Hidalgo M, Portero JM, Demyda‐Peyras S, Ortiz I, Dorado J (2014) Cryopreservation of canine semen after cold storage in a Neopor box: effect of extender, centrifugation and storage time. Vet Rec 175: 20-22.
• Johns A, Morris S, Edwards K, Quirino RL (2015) Asolectin from soybeans as a natural compatibilizer for cellulose‐reinforced biocomposites from tung oil. J Appl Polym Sci 132: 41833.
• Kim SH, Yu DH, Kim YJ (2010) Effects of cryopreservation on phosphatidylserine translocation, intracellular hydrogen peroxide, and DNA integrity in canine sperm. Theriogenology 73: 282-292.
• Lechner D, Aurich J, Schäfer-Somi S, Aurich C (2022) Effects of age, size and season on cryotolerance of dog semen – A retrospective analysis. Anim Reprod Sci 236: 106912.
• Ledvinka Z, Zita L, Klesalová L (2012) Egg quality and some factors influencing it: a review. Sci Agric Bohem 43: 46-52.
• Mahiddine FY, Kim MJ (2021) Overview on the antioxidants, egg yolk alternatives, and mesenchymal stem cells and derivatives used in canine sperm cryopreservation Animals 11: 1930.
• Miguel-Jimenez S, Del Alamo MM, Álvarez-Rodríguez M, Hidalgo CO, Peña AI, Muiño R, Rodríguez-Gil JE, Mogas T (2020) In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen. Anim Reprod Sci 215: 106315.
• Nguyen VV, Ponchunchoovong S, Kupittayanant S, Kupittayanant P (2019). Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm. Vet Med Sci 5: 345-360.
• Niżański W, Partyka A, Rijsselaere T (2012) Use of fluorescent stainings and flow cytometry for canine semen assessment. Reprod Domest Anim 47: 215-221.
• Palacin-Martinez C, Anel-Lopez L, Alvarez M, Neila-Montero M, Montes-Garrido R, Soriano-Úbeda C, de Paz P, Anel L, Riesco MF (2024) The characterization of CellROX™ probes could be a crucial factor in ram sperm quality assessment. Front Vet Sci 11: 1342808.
• Paz M, FrancoTrecu V, Szteren D, Costábile A, Portela C, Bruno A, Moratorio G, Moreno P, Cristina J (2024) Understanding the emergence of highly pathogenic avian influenza A virus H5N1 in pinnipeds: an evolutionary approach. Virus Res 350: 199472.
• Peña FJ, Núñez-Martínez I, Morán JM (2006) Semen technologies in dog breeding: an update. Reprod Domest Anim 41: 21-29.
• Pignataro TA, de Araújo JM, Silva AB, Freitas ML, Teixeira HC, Pivato I, Oliveira RA(2020) Comparison of extenders and storage temperature in chilling canine semen. Ciênc Anim Bras 21: e-52499.
• Piomboni P, Focarelli R, Stendardi A, Ferramosca A, Zara V (2012) The role of mitochondria in energy production for human sperm motility. Int J Androl 35: 109-124.
• Plaza Davila M, Martin Muñoz P, Tapia JA, Ortega Ferrusola C, Balao da Silva CC, Peña FJ (2015) Inhibition of mitochondrial complex I leads to decreased motility and membrane integrity related to increased hydrogen peroxide and reduced ATP production, while the inhibition of glycolysis has less impact on sperm motility. PLoS One 10: e0138777.
• Pulkowska-Bluj O, Trzcińska M (2025) Collection, preservation, and assessment of canine epididymal semen – current knowledge and future challenges. Ann Anim Sci 25: 1429-1438.
• Rigau T, Rivera M, Palomo MJ, Fernández-Novell JM, Mogas T, Ballester J, Peña A, Otaegui PJ, Guinovart JJ, Rodríguez-Gil JE (2002) Differential effects of glucose and fructose on hexose metabolism in dog spermatozoa. Reproduction 123: 579-591.
• Rijsselaere T, Van Soom A, Maes D, de Kruif A (2002) Effect of centrifugation on in vitro survival of fresh diluted canine spermatozoa. Theriogenology 57: 1669-1681.
• Sánchez-Calabuig MJ, Maillo V, Beltrán-Breña P, de la Fuente Martínez J, Galera-Carrillo S, Pérez-Gutiérrez JF, Pérez-Cerezales S (2017) Cryopreservation of canine sperm using egg yolk and soy bean based extenders. Reprod Biol 17: 233-238.
• Schäfer-Somi S, Colombo M, Luvoni GC (2022) Canine spermatozoa – predictability of cryotolerance. Animals 12: 733.
• Volpe S, Leoci R, Aiudi G, Lacalandra GM (2009) Relationship between motility and mitochondrial functional status in canine spermatozoa. Reprod Domest Anim 44: 275-278.
• Zakošek Pipan M, Casal ML, Šterbenc N, Virant Klun I, Mrkun J (2020) Vitrification using soy lecithin and sucrose: a new way to store the sperm for the preservation of canine reproductive function. Animals 10: 653.

Date

17.03.2026

Type

Article

Identifier

DOI: 10.24425/pjvs.2026.158499