Szczegóły

Tytuł artykułu

Comparisons of the hypo-osmotic swelling and water tests to assess functional membrane integrity (FMI) of dog sperm from the sperm-rich fractions (SRFs) and whole ejaculates (WEs)

Tytuł czasopisma

Polish Journal of Veterinary Sciences

Rocznik

2026

Wolumin

Vol. 29

Numer

No. 1

Autorzy

Afiliacje

Strzeżek, R. : Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland ; Fraser, L. : Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

Słowa kluczowe

dog ; HOS test ; sperm ; water test

Wydział PAN

Nauki Biologiczne i Rolnicze

Zakres

121–125

Wydawca

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliografia

  • Abah KO, Ligocka-Kowalczyk Z, Partyka A, Prochowska S, Fontbonne A, Niżański W (2025) Deciphering the mechanism of sperm cell aging - Functional, structural, and kinematic characteristics in fresh semen of mature and aged dogs. Anim Reprod Sci 274: 107798.
  • Bahamondes L, Fazano F, De Lucio MA, Neves PA, Bottcher Luiz F, Lorenzetti GB (2001) Evaluation of human sperm membrane integrity using the water test and the hypoosmotic test. Andrologia 33: 75-77.
  • Bland JM, Altman DG (2010) Statistical methods for assessing agreement between two methods of clinical measurement. Int J Nurs Stud 47: 931-936.
  • Check JH, Check DL, Bollendorf A (2023) Hypo-osmotic swelling test and male factor. Reprod Med 4: 118-132.
  • Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70: 219-228.
  • Hishinuma M, Sekine J (2003) Evaluation of membrane integrity of canine epididymal spermatozoa by short hypoosmotic swelling test with ultrapure water. J Vet Med Sci 65: 817-820.
  • Lomeo AM, Giambersio AM (1991) ‘Water-test’: a simple method to assess sperm-membrane integrity. Int J Androl 14: 278-282. 
  • Prochowska S, Niżański W, Fontbonne A (2022) Hypo-osmotic swelling test (HOST) for feline spermatozoa: the simplified procedure and the aspect of sperm morphology. Animals (Basel) 12: 903.
  • Strzeżek R, Fraser L (2009) Characteristics of spermatozoa of whole ejaculate and sperm-rich fraction of dog semen following exposure to media varying in osmolality. Reprod Biol 9: 113-126.
  • Quintela AT, Oliveira IR, Souza AO, Gusmao AL, Silva AR (2010) Water-induced hypo-osmotic test for the evaluation of canine sperm membrane integrity. Anim Reprod 7: 70-74.

Data

17.03.2026

Typ

Short communication

Identyfikator

DOI: 10.24425/pjvs.2026.158504 ; ISSN 1505-1773
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