Life Sciences and Agriculture

Journal of Plant Protection Research

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Abstract

Compost piles serve as important habitats for various insect groups, including decomposers, predators, and parasitoids. While drosophilid fruit flies play a crucial role in organic matter recycling, the variation of their abundance and diversity in composters remains poorly understood. This study examines fruit fly assemblages across three compost localities in Poland: two orchards in Dąbrowice and Nowy Dwór-Parcela, and a vegetable-fruit farm in Skierniewice. Insects, collected using sweep netting and traps, varied in abundance. Dipteran flies were the most prevalent, representing 25 families, followed by beetles, bees, and wasps. Thrips and earwigs were the least represented. With 16 species from three genera, drosophilids were the predominant group, making up 95.6% of all specimens. These included seven cosmopolitan species, namely Drosophila melanogaster, D. hydei, D. immigrans, D. buskii, D. repleta, D. simulans, and Scaptomyza pallida, and three alien species, D. suzukii, Chymomyza amoena, and D. triauraria, the latter being a new Asian species recently reported in Poland and Europe. In addition to D. suzukii, another significant pest, Carpophilus hemipterus, was also recorded in the compost piles. Most species were fruit breeders or decaying plant material inhabitants, while others were mycetophagous (D. testacea, D. transversa, D. phalerata, D. funebris) or frugivorous-fungivorous (D. subobscura). Shannon-Wiener diversity indexes ranged between 1.1 and 1.4 across three localities, with the highest drosophilid diversity found at the Skierniewice farm. Drosophila melanogaster was the most numerous fruit fly at all the examined compost piles, while the relative abundance of other species depended on the composter site. These findings emphasize composters as underexplored hotspots for drosophilids, directing further study of their ecological niches and the potential presence of pest species.
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Authors and Affiliations

Katarzyna Michalska
1
Muhammad Arslan Ibrahim
1
Mariusz Martyka
1
Marcin Studnicki
2
Grażyna Soika
3

  1. Department of Plant Protection, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Warsaw, Poland
  2. Department of Biometry, Institute of Agriculture, Warsaw University of Life Sciences, Warsaw, Poland
  3. Department of Plant Protection, The National Institute of Horticultural Research, Skierniewice, Poland
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Abstract

The present research reports the first record in Poland of the alien invasive and quarantine species Chaetosiphon (Pentatrichopus) fragaefolii (Cockerell, 1901) – the strawberry aphid. Native to North America, it is a critical pest and viral vector associated with strawberry crops. This study provides valuable insights into the genetic and morphological characteristics of the strawberry aphid. By integrating morphological and molecular analyses, the taxonomic resolution has been improved and the understanding of this economically important species has been deepened, particularly regarding its genetic diversity, distribution, and potential invasion routes. For the first time, scanning electron microscopy (SEM) analyses were performed to elucidate the general morphology, chaetotaxy, and sensilla of the antennae and mouthparts, contributing to improved knowledge related to both natural and chemical control strategies.
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Authors and Affiliations

Mariusz Kanturski
1
Minho Lee
2
Andżela Glumac
1
Seunghwan Lee
2

  1. Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland
  2. Insect Biosystematics Laboratory, Department of Agricultural Biotechnology, Research Institute of Agricultural and Life Sciences, Seoul National University, Seoul, Korea (South)
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Abstract

The use of fungicides to limit the development of soybean mycoses in modern agrocenoses is a common measure. At the same time, the use of symbiotic and phosphate-mobilizing bacteria increases the productivity and quality of the crop. The combination of biological agents and fungicides can have different effects and requires additional research. This study aimed to investigate the rhizosphere microbiome, mycosis resistance and soybean productivity under combined seed treatment with bacterial inoculants and fungicides. Pre-sowing treatment of soybean seeds was carried out with fungicides Maxim Star 025 FS or Kinto Duo, and 12 hours later the seeds were inoculated with the complex bioformulation Ecovital based on Bradyrhizobium japonicum and Bacillus megaterium. The effect of the integrated seed treatment on the rhizosphere microbiome (by high-throughput sequencing), the level of mycosis development and soybean productivity was determined. Under the combined use of biological and chemical preparations, the biodiversity of the rhizosphere microbiome improved, the relative abundance of fungicide-resistant phyla increased, and the number of sensitive taxa decreased. The low abundance of native rhizobia populations indicated the need for pre-sowing inoculation of soybean seeds. The combined use of fungicides and inoculation of seeds with Ecovital contributed to improved effectiveness of plant protection against ascochitosis and septoria and to an increase in soybean yield.
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Authors and Affiliations

Galyna Iutynska
1
Liudmyla Tytova
1
Svitlana Vozniuk
1
Nadiia Shevchuk
1
Liudmyla Bilyavska
1

  1. Department of General and Soil Microbiology, Zabolotny Institute of Microbiology and Virology of NASU, Kyiv, Ukraine
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Abstract

Crop yields depend not only on genetic traits, agronomic practices, and weather but also on effective crop protection. European agriculture aims to reduce the use of harmful chemicals while maintaining yields. Studies show that adjuvants can reduce the required doses of plant protection products. While their role in herbicide and fungicide applications is well documented, research on plant growth regulators remains limited. Field trials were conducted at the Institute of Plant Protection – National Research Institute in Poland to evaluate the impact of reduced doses of growth regulators, along with adjuvants, on the growth and yield of winter wheat. The study aimed to evaluate the potential and effectiveness of combining the plant growth regulators mepiquat chloride and prohexadione calcium with various adjuvants and additives in winter wheat under field conditions. The experimental treatments included a mixture of mepiquat chloride with prohexadione calcium (Medax Top 350 SC) applied together with citric acid; fertilizers such as urea and ammonium sulfate; and adjuvants including heptamethyltrisiloxane-modified polyalkylene oxide (Slippa), 76% paraffin oil (Atpolan 80 EC), 80% rapeseed oil fatty acid methyl esters, surface-active agents (surfactants), and a pH buffer (Atpolan BIO 80 EC), as well as ammonium sulfate ((NH4)2SO4 – 40%), a cationic surfactant (20%), and triethanolamine (5%) (AS 500 SL). The studies showed that it is possible to achieve the same results using half the standard doses of regulators, combined with adjuvants, as when using full doses. This also applied to plant height as well as qualitative and quantitative grain parameters. Additionally, the findings demonstrated that the effect of mepiquat chloride combined with prohexadione calcium and adjuvants varied depending on weather conditions during crop growth.
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Authors and Affiliations

Kinga Matysiak
1
Wojciech Miziniak
2
Jan Bocianowski
3
ORCID: ORCID
Jolanta Kowalska
4
ORCID: ORCID

  1. Department of Weed Science and Plant Protection Technique, Institute of Plant Protection – National Research Institute, Poznań, Poland
  2. Regional Experimental Station in Toruń, Institute of Plant Protection – National Research Institute, Toruń, Poland
  3. Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland
  4. Department of Organic Farming and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, Poland

Instructions for authors

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

General information for preparing a manuscript

All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

Original article

The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

Tables, Figures, Phothographs, Drawings

Tables and figures should be uploaded as separated files at the submission stage. Their place in the manuscript should be clearly indicated by authors. Colour figures are accepted at no charge for the electronic version. In the hardcopy version of the journal, colour figures cost (65,5 € per one colour page). When attaching files please indicate if you want colour only in the online version or in both the online and the hardcopy. Photographs and RGB bitmaps should be provided in JPG or TIFF file format. They must have no less than 300 dpi resolution. The text column should be 8 cm wide and they must be at least 1000 pixels wide. Please send original (not resized) photograph(s), straight from a digital camera, without any text descriptions on the photo. Bitmaps combined with text object descriptions should be provided in MS Word or MS Powerpoint format. Text objects using Arial font-face should be editable (changing font-face or font size). Drawings should be provided in MS Word, MS Powerpoint, CorelDRAW or EPS file format and stored with original data file. Text objects using Arial font-face should be editable (changing font-face or font size). Charts (MS Excel graphs) should be provided in MS Excel file format, and stored with original MS Excel data file without captions but with the number of the figure attached. Please do not use bitmap fills for bar charts. Use colour fills only if necessary. Captions and legends should be added at the end of the text, referred to as "Fig." and numbered consecutively throughout the paper.

Rapid communications

Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

Reviews

Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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