Life Sciences and Agriculture

Journal of Plant Protection Research

Content

Journal of Plant Protection Research | 2025 | vol. 65 | No 1

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Abstract

Effective management of plant fungal pathogens is crucial for minimizing economic and environmental impacts of crop diseases in agricultural production. It plays a major role in providing healthy and nutritious food, maintaining human and animal well-being, and maintaining an environmental balance in agroecosystems. These goals agree with agroecology and Integrated Pest Management (IPM). Agroecology integrates ecological principles with agriculture and offers a holistic and environmentally friendly approach to fungal disease management. IPM focuses on prevention and protection against pests and diseases, involving environmentally safe agricultural practices, cultivating resistant plant varieties, and promoting agrobiodiversity. The authors aimed to provide a comprehensive and concise overview of the key components of IPM in sustainable agriculture including recent developments in electronic tools helping farmers to make optimal economic and environmental decisions. While maintaining agroecology principles there is a particular focus on the significance of plant resistance to major pathogens, breeding technologies, effective crop management practices, and non-chemical fungal management. Agroecological approaches to fungal plant pathogen management prioritize the long-term health of agricultural ecosystems, contributing to the overall biodiversity and sustainability of farming systems. To illustrate the practical application of these principles, the AgroVariety application (app), developed for farmers, was used to discuss the role of specialized applications in decision-making for environmentally friendly and cost-effective plant production. This tool emphasizes combining different IPM techniques, with specific emphasis on methods that are least harmful to the environment and tailored to control particular pathogens.
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Authors and Affiliations

Elżbieta Czembor
1
ORCID: ORCID
Anna Tratwal
2
ORCID: ORCID
Juliusz Pukacki
3
Marcin Krystek
3
Jerzy Henryk Czembor
1

  1. Plant Breeding and Acclimatization Institute – National Research Institute, Radzikow, Poland
  2. Department of Monitoring and Signalling Agrophages, Institute of Plant Protection – National Research Institute, Poznań, Poland
  3. Poznan Supercomputing and Networking Center, Poznan, Poland
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Abstract

The Solanaceae family includes many species of plants with high nutritional and medicinal value. Plants in this family have evolved towards diversifying specialized (secondary) metabolism to adapt to adverse conditions, and a few of them have been used as model plants in the study of plant defense. 2-Methylketones are insecticidal compounds that accumulate in certain plants, particularly in wild tomato Solanum habrochaites f. glabratum – a Solanaceae member. 2-Methylketones are the decarboxylated products of 3-ketoacids generated through the hydrolysis of 3-ketoacyl-ACPs by methylketone synthase 2 (MKS2). In this study, we isolated one of the four MKS2 homolog genes from the cultivated potato Solanum tuberosum and designated it as StMKS2-3. A combined in silico approach including sequence alignment, phylogenetic assessment, 3D structural modeling and RNA-sequencing data analysis was performed to exploit the functional properties of this gene. The encoded protein possesses the conserved Aspartate and functional domain characteristic of single Hotdog-fold thioesterases, and it shares high similarity in sequence and structure with SlMKS2a of the cultivated tomato (S. lycopersicum). This suggests that, like SlMKS2a, StMKS2-3 could act as a 3-ketoacyl-ACP thioesterase. According to RNA-seq data, StMKS2-3 exhibited higher expression than the other three StMKS2 genes in most tissues across different potato S. tuberosum cultivars. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that StMKS2-3 was expressed in multiple potato tissues, both aerial and root parts, but the highest expression was in tubers and sprouts. Furthermore, this gene appeared to be transcriptionally induced in response to salinity, drought, and Phytophthora infestans infection, supporting a possible role for StMKS2-3 in the response of potato S. tuberosum to such stress.
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Authors and Affiliations

Huong Thi Diem Tran
1
Thuong Thi Hong Nguye
1
ORCID: ORCID

  1. Faculty of Biology and Biotechnology, University of Science, Vietnam National University,Ho Chi Minh City, Nguyen Van Cu, Viet Nam
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Abstract

Sustainable agriculture is a management system based on the use of agricultural practices that contribute to maintaining the natural environment in good condition. It allows for the rational use of its resources, and minimizing losses for society while maintaining profitability of agricultural production. An important feature of a sustainable agricultural system is the moderate use of industrial agricultural production products, including plant protection products, which in practice should be closely matched to the type and strength of the threat occurring in agricultural crops. The cost of plant protection products per 1 ha of crops is an important indicator of the intensity of agricultural production, which as a result of growing competitive pressure, is still increasing, especially in areas with favorable natural conditions for its development. In this context, it is therefore important to emphasize the strong current aim and readiness of the European Union (EU) to restrict the use of plant protection products in agriculture. This is referred to as the “farm to fork” strategy, which is an important element of the European Green Deal. Currently, the European Commission (EC) is taking a number of legislative steps to reduce the overall use of plant protection products. However, the question arises whether and to what extent Polish agriculture can participate in the ambitious EU goal to reduce them. To try to answer this question, an assessment was made of the amounts and structure of the consumption of chemical plant protection products in Poland, and compared to other EU countries. It was also important to compare the costs of plant protection products per 1 ha of crops on farms in Poland, with similar farms in other European Union countries, while taking into consideration the diverse natural farming conditions in which they operate.
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Authors and Affiliations

Marek Zieliński
1
ORCID: ORCID
Arkadiusz Zalewski
2
ORCID: ORCID
Sylwia Łaba
3
ORCID: ORCID

  1. Department of Economics of Agricultural and Horticultural Holdings, Institute of Agricultural and Food Economics – National Research Institute, Warsaw, Poland
  2. Department of Agricultural Markets and Quantitative Methods, Institute of Agricultural and Food Economics – National Research Institute, Warsaw, Poland
  3. The Institute of Environmental Protection – National Research Institute, Warsaw, Poland
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Abstract

Endophytic Beauveria bassiana (Balsamo-Crivelli) Vuillemin can promote plant growth and health and protect them against herbivores. Two endophytic strains of B. bassiana, BS195 (isolated from soil) and BNE20 (isolated from cucumber), were used by foliar spray and root soaking to evaluate B. bassiana ability to colonize cucumber plants and promote their growth under stressful greenhouse conditions in two separate experiments, as well as its efficacy against Liriomyza sativae Blanchard in a controlled setting. The results showed that the effects significantly depended on the inoculation method and fungal strain. Both B. bassiana strains colonized endophytically the tissues of all cucumber plant parts 30 days post-inoculation, with root soaking being significantly better than foliar spray. The present study showed that the application of B. bassiana BS195, mainly through root soaking, en­hanced many growth and health parameters, including plant height, root length, number of leaves, leaf area, fresh and dry weight, the content of dry matter, and the total phenolic content. Cucumber plant treatment with B. bassiana significantly reduced the infestation, severity, number of pupae, and adult emergence of L. sativae after 35 and 51 days of adult release with greater efficiency with the root soaking method. We conclude that introducing B. bassiana through root soaking seems to be effective in stimulating plant growth, and can be a promising technique in controlling L. sativae populations on cucumber plants.

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Authors and Affiliations

Lobna Rajab
1
ORCID: ORCID
Ahmad M. Dayoub
1
ORCID: ORCID
Ibtisam Gazal
1
Mohammad Ahmad
1

  1. Department of Plant Protection, Faculty of Agriculture, Tishreen University, Latakia, Syria
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Abstract

Climate change has a great influence on weed growth and susceptibility of weeds to her­bicides. This study determined the effect of six herbicides on three weed species under different CO2 concentrations and temperature levels. The weeds in the study were: (i) wild oat (Avena fatua), (ii) lambsquarter (Chenopodium album), and (iii) wild mustard (Sinapis arvensis). The herbicides used in this study were: (i) 240 g ꞏ l–1 clodinafop-propargyl, (ii) 3% mesosulfuron-methyl + 0.6% iodosulfuron-methyl sodium + 9% mefenpyr-diethyl, (iii) 40 g ꞏ l–1 nicosulfuron, (iv) 480 g ꞏ l–1 glyphosate isopropylamine salt, (v) 75% tribenu­ron methyl and (vi) 3% mesosulfuron-methyl + 0.6% iodosulfuron-methyl sodium + 9% mefenpyr-diethyl + 300 g ꞏ l–1 bromoxynil + 300 g ꞏ l–1 MCPA. The study was carried out in a fully automated greenhouse which could be adjusted with desired CO2 concentra­tion and temperature. The weeds were exposed to three different temperatures (day/night 26/16 ± 1, 29/19 ± 1 and 32/22 ± 1°C) and CO2 (400 ± 50, 600 ± 50 and 800 ± 50 ppm) levels. A temperature of 26/16 ± 1°C plus a CO2 level of 400 ± 50 ppm was considered as the control. Results showed that 26/16°C × 800 ppm CO2 produced the highest plant length (65.05 cm), plant fresh weight (7.42 g) and plant dry weight (1.31 g) for A. fatua. Similarly, for S. arvensis, the same treatment showed the highest plant length (31.63 cm), plant fresh weight (23.99 g) and plant dry weight (1.82 g) while for C. album, different climatic condi­tions did not show a significant effect on the growth of this weed. The ED50 values of herbi­cides for controlling A. fatua, C. album and S. arvensis increased (112.8, 0.6 and 199.4) with an increase in temperature and CO2 levels, respectively. It is predicted that the control of some weeds will be difficult in the climate change that includes an increase in temperature and carbon dioxide in the future.

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Authors and Affiliations

Nihat Tursun
1
ORCID: ORCID
Khawar Jabran
2
ORCID: ORCID
Olcay Bozdogan
1
Yucel Karaman
1

  1. Plant Protection, Faculty of Agriculture, Malatya Turgut Ozal University Malatya, Turkey
  2. Plant Production and Technologies, Faculty of Agricultural Sciences and Technologies, Nigde Omer Halisdemir University, Nigde, Turkey
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Abstract

Quantification and assessment of disease symptoms are important elements of plant disease management systems and are required to assist with making decisions on the choice of protective agents to be applied to crops or for screening plant genotypes for the development of resistant varieties. Traditional methods of identifying and quantifying disease severity are cumbersome, involving visual assessment tools or scales, and rating of plants at a point in time. Visual assessment is prone to human bias and error, thereby reducing the efficiency and accuracy of this method. In this study, we developed a smartphone camera- -based image recording, processing, and assessment tool for measurement of symptoms of early and late blight, and bacterial leaf spot diseases in sweet pepper caused by Alternaria solani, Phytophthora infestans, and Xanthomonas campestris pv. vesicatoria, respectively. Sweet pepper or bell pepper is a major vegetable crop grown in the Caribbean region, but production is severely affected by plant diseases, most important of which include foliar infections by fungi and bacteria that cause major losses in fruit yield. This research utilized smartphone captured images of leaf specimens for severity measurement and classification of diseases. The steps involved were color space conversions, detection of leaf area by Otsu’s method, and thresholding for foliar diseased area detection and quantification. Gray-Level Co-occurrence Matrix (GLCM) extracted the texture features from the diseased area of leaves. These features are trained and classified by various machine learning classifiers including trees, rule-based and Bayes models. Application of decision trees and rule-based classifier models achieved 98% accuracy individually, while Bayes model achieved 86% accuracy. The image input into the above classifier models resulted in fast and accurate identification of the diseases by matching the features of trained images of disease symptoms. This method could work well for leaves collected from field-grown plants as well as from inoculated greenhouse plants.
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Authors and Affiliations

Vijayanandh Rajamanickam
1
ORCID: ORCID
Adesh Ramsubhag
2
Jayaraj Jayaraman
2

  1. Department of Computing and Information Technology, Faculty of Science and Technology, The University of the West Indies St. Augustine Campus, Trinidad and Tobago
  2. Department of Life Science, Faculty of Science and Technology, The University of the West Indies, St. Augustine Campus, Trinidad and Tobago
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Abstract

Phytoplasma subgroups 16SrIV-A and -D are the agents associated with two diseases that significantly threaten palm cultivation in the Americas, namely lethal yellowing (LY) and Texas Phoenix palm decline (TPPD), respectively. Recently, in Puebla State, Mexico, several Phoenix canariensis Chabaud and Syagrus romanzoffiana (Cham.) Glassman palms used as ornamentals began to show symptoms resembling those of TPPD and LY. Therefore, the present study aimed to demonstrate the spread of group 16SrIV phytoplasmas to Puebla, Mexico. Ten symptomatic individuals of both palms were sampled and a nested PCR assay with primer pair P1/P7 followed by LY16Sf/LY16Sr was performed to detect phytoplasma presence. A fragment of about 1.4 kb was amplified in six palms, three (of four) P. canar­iensis and three (of six) S. romanzoffiana. Sequence analysis of the amplicons revealed that the phytoplasma isolates from Puebla were members of group 16SrIV, subgroups – A (one isolate from P. canariensis) and -D (rest of isolates). This study reports the first occurrence of TPPD and LY on ornamental palm species in the state of Puebla, Mexico.

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Authors and Affiliations

Pablo José Palma-Cancino
1
ORCID: ORCID
Carlos Fredy Ortiz-García
1
ORCID: ORCID
Jesús Francisco López-Olguín
2
ORCID: ORCID
Agustín Aragón-García
3
ORCID: ORCID

  1. Campus Tabasco, Postgraduate College, Cárdenas, Tabasco, Mexico
  2. Herbarium and Botanical Garden, Vice-rectorate for Research and Postgraduate Studies, Meritorious Autonomous University of Puebla, Puebla, Mexico
  3. Agroecology Center, Institute of Sciences, Meritorious Autonomous University of Puebla, Puebla, Mexico
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Abstract

At the current level of applied entomology, comprehensive research on the species composition and population dynamics of the insect community in crops is of the utmost importance. Comprehensive studies on sorghum agroecosystems in Bulgaria have been scarce and have necessitated, necessitating a need for new and up-to-date developments. The study investigated the species composition and population dynamics of the main insect species in Bulgaria’s sorghum. Insect diversity was represented by 51 insect species belonging to 17 families and 46 genera. Harmful species made up 70.6% of the total population density of insect representatives, while beneficial (predators) – 29.4%. The insect composition included two aphid species, 17 species of cicadas, 11 species of bugs, 17 species of beetles, and four thrips species. Aphids and cicadas were some of the most important insect pests of sorghums in Bulgaria. Temperature changes affected the life characteristics of insects and ultimately determined population growth rates. Various factors like global warming, changing cropping patterns, and adoption of technologies alter insect abundance, distribution, and pest-associated losses. Work, related to the survey and surveillance of sorghum insect pests should be regularly conducted to gain proper knowledge of changing trends of insect pests, associated losses, and timely management.
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Authors and Affiliations

Ivelina Nikolova
1

  1. Department Selection and Technology, Institute of Forage Crops, General Vladimir Vazov, Pleven, Bulgaria
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Abstract

Root rot of sugar beet (Beta vulgaris L.), caused by Rhizoctonia solani anastomosis group AG 2-2 IIIB is responsible for significant crop losses in North Dakota and Minnesota, USA. Understanding the association between plant age and inoculum density with disease sever­ity of sugar beet cultivars is a prerequisite to properly screen for varietal resistance. There­fore, investigations were conducted to determine the responses of 4-, 6-, and 8-week-old plants in seven commercial sugar beet cultivars to inoculum densities of one, two, and three grains of R. solani-colonized barley in a greenhouse and with three corresponding levels of colonized barley, mycelial plugs, and sclerotia in field experiments. Under greenhouse con­ditions, disease severity was greatest before plants reached six weeks of age (p = 0.05). There was a positive linear relationship between the density of the inoculum and disease severity. All seven cultivars were equally susceptible (p > 0.05) to R. solani. Interactions between cul­tivars and plant age and between plant age and intensity of inoculum were not significant (p > 0.05). Field experiments showed that the density of inoculums was significant (p < 0.001), and the disease severity was highest in plants inoculated with three colonized barley seeds per plant compared to doses of other inoculum types.

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Authors and Affiliations

M.Z.R. Bhuiyan
1
ORCID: ORCID
Luis Del Río Mendoza
1
Dilip K. Lakshman
2
ORCID: ORCID
Aiming Qi
3
M.F.R. Khan
1 4

  1. Department of Plant Pathology, North Dakota State University, Fargo, North Dakota, USA
  2. Molecular Plant Pathology Laboratory, USDA-ARS, Beltsville, Maryland, USA
  3. School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Haltfield, UK
  4. University of Minnesota, St. Paul, Minnesota, USA
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Abstract

Agricultural production has a direct impact on the environment, both by consuming natural resources and by generating hazards in the form of emissions of various substances into the environment. Increased demand for plant products on global food markets contributes to heightened environmental pressure on agriculture. Agriculture, along with other sectors, should adhere to sustainability principles. Ongoing global development hinges on achieving a balance between economic growth and natural resource conservation. To fulfill the goals of sustainable development, agriculture should strive to minimize energy and natural resources consumption, thereby reducing its environmental impact. In the above context, research on the environmental effects of different agricultural production systems is needed. The aim of this study was to assess the environmental effects of two cultivation systems, conventional and organic, throughout the life cycles of winter wheat and potato production. The research employed a life cycle assessment (LCA) methodology from cradle to farm gate for assessing environmental impacts of crop cultivation across different farming systems, with respect to the functional unit of 1 tonne. Organic farming was shown to have lower environmental impacts than a conventional production system. The results confirm the sustainable nature of organic farming and its ability to mitigate the effects of farming activities. The LCA of conventional wheat and potato production showed that fertilizer application was the main environmental concern, highlighting the need to optimize fertilization to reduce environmental impacts. Furthermore, the results indicated that acidification and depletion of abiotic fossil fuel resources were significant environmental threats within the systems analyzed.
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Authors and Affiliations

Małgorzata Holka
1
Jolanta Kowalska
1
ORCID: ORCID

  1. Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection − National Research Institute,Poznań, Poland

Instructions for authors

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

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All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

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The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

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Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

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Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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