Life Sciences and Agriculture

Journal of Plant Protection Research

Content

Journal of Plant Protection Research | 2026 | vol. 66 | No 1

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Abstract

Mixed pesticides are promising alternative approaches for insect pest management and could reduce the risk to the environment. Therefore, their efficiency against pests and their residues in the environment should be investigated. In this study, the efficacy of pymetrozine, cyantraniliprole, and their mixture against Aphis gossypii Glover and their residues in cucumber (Cucumis sativus L.) were studied. The results showed that pymetrozine and cyantraniliprole alone or in combination were more effective than acetamiprid insecticide for reducing A. gosspyii populations up to 15 days following application. Additionally, a residual analytical method for insecticide determination in cucumber fruits, leaves, and soil was optimized and validated. The tested insecticides’ residue extraction was carried out using the QuEChERS method, and the determination was achieved using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The recovery tests were studied at three spiking levels of low concentration (0.01, 0.05, and 0.1 mg · kg–1) with acceptable recovery between 71 and 101% and good precision and expanded uncertainty up to ± 20%. The half-life times of the tested formulations ranged from 8.19 to 10.6 days, while the pre-harvest intervals (PHI) ranged from 8.8 to 23.8 days.
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Authors and Affiliations

Nawal AbdulAziz Alfuhaid
1
Hassan A. El-Gammal
2
Nourhan A. El-Said
3
EL-Desoky S. Ibrahim
3
Moataz A.M. Moustafa
3

  1. Department of Biology, College of Science and Humanities, Prince Sattam Bin Abdulziz University, Al-Kharj, Saudi Arabia
  2. Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Foo, Agricultural Research Center, Ministry of Agriculture and Land Reclamation, El-Dokki, Giza, Egypt
  3. Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt
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Abstract

Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceris (Foc), is a major constraint in chickpea production in Turkey, leading to yield losses of up to 50%. This study aimed to characterize the morphological and molecular traits of Foc isolates and evaluate the antagonistic efficacy of three Trichoderma species under in vitro and greenhouse conditions. A total of 20 Foc isolates were obtained from diseased chickpea plants in Tarsus, Mersin, of which 10 were confirmed by PCR amplification of the ITS region. The isolates showed variation in colony morphology, spore type, and pathogenicity, with Foc8, 10, 14, 15 and 20 being the most virulent. Dual culture assays demonstrated that Trichoderma harzianum (T3) had the highest antagonistic activity, inhibiting mycelial growth of Foc isolates by up to 68%. Headspace GC–MS analysis indicated that T. harzianum VOCs were rich in antifungal ketones, in contrast to the alcohol- and acid-dominated VOCs of Foc. Greenhouse experiments revealed that T. harzianum not only suppressed disease development but also significantly enhanced chickpea growth by increasing root biomass, seed weight, and relative water content. These findings highlight T. harzianum as a promising biocontrol agent for the sustainable management of Fusarium wilt in chickpeas. Further field validation and formulation development are recommended to support its use in integrated disease management programs.
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Authors and Affiliations

Abdulkreem Alsalmo
1
ORCID: ORCID
Mukaddes Kayim
1
ORCID: ORCID
Hira Nawaz
2
Tarq Hamijo
3
Nesibe Ebru Kafkas
2
ORCID: ORCID

  1. Department of Plant Protection, Çukurova Unıversity, Balcali-01330, Adana, Turkey
  2. Department of Horticulture, Çukurova Unıversıty, Balcali-01330, Adana, Turkey
  3. Department of Biotechnology, Çukurova Unıversity, Balcali-01330, Adana, Turkey
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Abstract

Phelipanche aegyptiaca is an obligate root-parasitic plant of the Orobanchaceae family that comprises the most threatening weed for major crops in regions with a Mediterranean climate zone, as well as in Asia, southern and Eastern Europe. The pre-attachment stage is crucial for its successful attachment and parasitism, making this phase a key target for biological control strategies. In this study, the effect of 79 fungal isolates, including 49 Fusarium and 30 Rhizoctonia, on the pre-attachment stages of P. aegyptiaca were investigated. According to the experimental results, the Fusarium isolates inhibited the seed germination by the rate of 17.9 to 97.1%, while the disease severity values were between 21.3 and 99.8%. Approximately 40% of the 49 Fusarium isolates exhibited high inhibition effects (70−100%) and around 70% of the isolates caused high disease severity (70−100%) on the radicle. A positive correlation was observed between the inhibition rate and disease severity for the Fusarium isolates. Moreover, 15 Fusarium isolates displayed high effects in both parameters simultaneously. In contrast, the Rhizoctonia isolates exhibited seed inhibition rates ranging from 1.2 to 37.6% and disease severity values between 0 and 66.0%, with none showing high effects on any pre-attachment stages. These findings suggest that the Fusarium isolates have a great potential to use biocontrol agents against P. aegyptiaca, while the Rhizoctonia isolates have less, which may be due to differences in their secondary metabolite profiles or other pathogenicity-related traits. These findings pave the way for future research to optimize Fusarium-based biocontrol strategies and better understand the broader potential of these fungal isolates in managing P. aegyptiaca.
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Authors and Affiliations

Esra Cignitas
1
Gurkan Basbagci
1
ORCID: ORCID
Yasin Emre Kitis
2
ORCID: ORCID

  1. Plant Pathology, Bati Akdeniz Agricultural Research Institute, Boztepe, 07110, Antalya, Turkey
  2. Plant Pathology, Akdeniz University, Pınarbaşı, 07070, Antalya, Turkey
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Abstract

This study investigated the effects of Saccharopolyspora thermophila, a plant growth-promoting actinomycete (PGPA), on tomato plants (Suvarna 22) and its potential to mitigate Fusarium wilt disease. Saccharopolyspora thermophila was isolated from organic farm soil and molecularly identified through 16S rRNA sequencing. Seed treatment with S. thermophila significantly enhanced germination rates (83% vs 59% in control) and seedling vigor (625 vs 192 in control). Treated plants exhibited improved growth parameters, including increased plant height (83.3%), chlorophyll content (88.2%), and fruit yield (66.7%). Under pathogen challenge, S. thermophila-treated plants showed reduced disease incidence (37% vs 92% in untreated plants). Biochemical analyses revealed that S. thermophila treatment enhanced antioxidant enzyme activities (CAT, SOD, APX, POD) and maintained higher chlorophyll content under pathogen stress. The treatment also moderated pathogen-induced increases in lipid peroxidation and proline levels, indicating improved stress tolerance. Interestingly, S. thermophila treatment counteracted pathogen-induced starch depletion and moderated sucrose accumulation, suggesting a complex interplay in plant metabolism regulation. These findings demonstrated the potential of S. thermophila as a PGPA to enhance tomato plant growth, productivity, and resilience against Fusarium wilt disease. The study provides insights into the physiological and biochemical mechanisms underlying PGPA-mediated plant growth promotion and disease resistance, offering promising avenues for sustainable agricultural practices.
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Authors and Affiliations

Chaitra Hanumanthappa
1
Yathisha Neeragunda Shivaraj
2
Sudish Jogaiah
3
Sharathchandra Ramasandra Govind
2

  1. Department of Studies and Research in Environmental Science, Tumkur University, Tumakuru, India
  2. Department of Studies and Research in Biotechnology and Microbiology, Tumkur University, Tumakuru, India
  3. Department of Environmental Science, Central University of Kerala, Tejaswini Hills, Periye, India
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Abstract

Anthracnose disease, primarily caused by Colletotrichum species, poses significant challenges to fruit production, resulting in serious global economic losses. While chemical fungicides are effective, their environmental and health risks underscore the need for sustainable alternatives. This study evaluated the antifungal potential of Sacha inchi (Plukenetia volubilis) leaf ethanol extract against Colletotrichum gloeosporioides, C. scovillei, and C. acutatum. Gas Chromatography-Mass Spectrometry (GCMS) analysis identified 20 chemical constituents, including bioactive compounds such as unsaturated fatty acids and saturated fatty acids known for their antimicrobial properties. In vitro assays demonstrated a dose-dependent inhibition of mycelial growth, with complete suppression of C. gloeosporioides at 500 mg · l–1. The antifungal activity is likely attributed to the extract’s ability to disrupt fungal cell membranes and interfere with metabolic pathways. These findings support the potential of Sacha inchi leaf extract as a promising, eco-friendly alternative to synthetic fungicides in the management of anthracnose disease in fruit crops. Further research into its field application, synergistic effects, and mechanisms of action is warranted to enhance its integration into sustainable crop protection strategies.
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Authors and Affiliations

Farah Farhanah Haron
1
Siti Fairuz Yusoff
2
ORCID: ORCID
Fazhana Ismail
2

  1. Biological Control Programme, Agrobiodiversity and Environment Research Centre, Malaysian Agricultural Research and Development Institute, Serdang, Selangor, Malaysia
  2. Agricultural Science Department, Faculty of Technical and Vocational, Universiti Pendidikan Sultan Idris, Tanjong Malim, Perak, Malaysia
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Abstract

This study aimed to evaluate the effects of plant extracts of Capsicum annuum, Datura stramonium and Allium sativum and the fungus Beauveria bassiana on different developmental stages of Bemisia tabaci. The D. stramonium extract achieved the highest adult mortality rate of 92.4% at 4% w/v concentration after 96 hours of exposure, which was higher than A. sativum (85.7%) and C. annuum (76.3%). The isolated D. stramonium extracts demonstrated maximum lethal activity against the early developmental stages of B. tabaci, with second instar showing the highest vulnerability and mortality rate of up to 97.8%. B. bassiana exhibited maximum pathogenicity against B. tabaci life stages, specifically with the second instar nymphs showing the highest susceptibility at 93.6% mortality when treated with 1×108 conidia · ml–1. The combination of plant extracts with B. bassiana resulted in elevated mortality rates with all combinations demonstrating synergistic effects with co-toxicity factor (CTF) values > 20. Lethal potency emerged from the combination treatment of D. stramonium (1% w/v) and B. bassiana (1 × 106 conidia · ml–1), which reached a maximum adult mortality of 98.0% ± 2.1. Greenhouse trials showed that this combined treatment generated population reductions exceeding 90% across all life stages, including adult insects, nymphs, and eggs within 14 days. These findings confirm that both botanical extracts and B. bassiana are suitable components for developing sustainable Integrated Pest Management (IPM) programs due to their efficient and environmentally friendly insect pest control properties.
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Authors and Affiliations

Ammar Kareem Jasman
1
ORCID: ORCID
Ali Karim Slomy
1
Ayad Ismael Khaleel
1

  1. Agriculture College, Al-Qasim Green University, Iraq – Babel, 51006, Babel, Iraq
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Abstract

This work investigated the influence of nitrogen compounds on soil–plant–insect interactions. The study aimed to assess the effect that cultivation of two spring wheat cultivars (Triticum sphaerococcum Percival and Triticum persicum Vavilov) at various plant densities (400, 500 and 600 grains · m–2) under organic and conventional farming had on the abundance of mimetabolous insects in the context of selected chemical and biochemical soil properties. The soil was assayed for its content of organic carbon (SOC), total nitrogen (STN), nitrate (SNO3–) and activity of proteases (PRO) – an enzyme involved in N transformation. The aboveground part of spring wheat was assayed for the content of total nitrogen (TNC) and nitrate nitrogen (TNO3–). Spring wheat variety and plant density both determined the SOC and STN content. The tested soil was characterized by low SOC content. PRO activity was positively correlated with SOC and STN contents, which indicated the role of this enzyme in organic matter cycling. Thysanoptera, Aphididae and Miridae were found to prefer spring wheat grown under organic farming. However, the numbers of these pests did not pose a threat to the growth and development of the host plant. Hemimetabolous insects were far less abundant on T. persicum than on T. sphaerococcum.
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Authors and Affiliations

Joanna Lemanowicz
1
ORCID: ORCID
Robert Lamparski
2
Jarosław Pobereżny
3
ORCID: ORCID
Elżbieta Wszelaczyńska
3
ORCID: ORCID
Agata Bartkowiak
4
ORCID: ORCID
Małgorzata Szczepanek
3

  1. Department of Biochemistry, Faculty of Medicine, Bydgoszcz University of Science and Technology in Bydgoszcz, Bydgoszcz, Poland
  2. Department of Microbiology and Plant Ecology, Bydgoszcz University of Science and Technology in Bydgoszcz, Bydgoszcz, Poland
  3. Department of Agronomy and Food Processing, Bydgoszcz University of Science and Technology in Bydgoszcz, Bydgoszcz, Poland
  4. Department of Biogeochemistry, Soil Science, Irrigation and Drainage, Bydgoszcz University of Science and Technology in Bydgoszcz, Bydgoszcz, Poland
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Abstract

Twisted disease caused by Fusarium is one of the causes of the decline in shallot production in Indonesia. Disease management by adding Casuarina leaf litter and maize leaf litter during planting time has suppressed twisted disease incidence and increased shallot production in previous studies. Bacterial communities can influence disease suppression and increase plant production in soil. However, the effect of adding Casuarina and maize leaf litter on the soil bacterial community, and its impact on suppressing twisted disease is still unknown in shallot planting. In addition, no further studies have been conducted on the effectiveness of the application time of Casuarina and maize leaf litter. This study aimed to determine the best application time of leaf litter in suppressing twisted disease, the effect of changes in soil bacterial communities, and bacteria that act as core microbe, biomarkers, and keystone taxa. Results showed that adding Casuarina and maize leaf litter during planting time was more effective than adding Casuarina and maize leaf litter two weeks before planting. The diversity and abundance of bacteria in soil with the addition of Casuarina leaf litter (C) were higher than those of maize leaf litter (J). In addition, Pseudarthrobacter was found in group C, which acts as a core microbe, biomarker, and keystone taxa that may be able to suppress pathogens that cause twisted disease. Paenibacillus acts as a core microbe, biomarker, and keystone taxa in group J, which may increase shallot production. This study’s results will help develop effective twisted disease management strategies to control disease development.
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Authors and Affiliations

Novianti Ekaputri Rahmawati
1
ORCID: ORCID
Arif Wibowo
1
Ani Widiastuti
1
ORCID: ORCID
Siti Subandiyah
1
Stephen Harper
2

  1. Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Yogyakarta, Indonesia
  2. School of Agriculture and Food Sciences Faculty of Science, University of Queensland, South Wing, Brisbane, Australia
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Abstract

Tomato (Solanum lycopersicum L.) is the most commonly consumed vegetable in Nigeria, and synthetic pesticides are frequently used to protect tomatoes against pests and diseases. However, the environment and human health are negatively impacted by their use, prompting consideration of alternative, environmentally safe treatments. Lantana camara L. (commonly known as sage, family Verbenaceae), a commonly occurring plant, has shown promise as a natural alternative for controlling tomato pests and may serve as an eco-friendly substitute for synthetic pesticides. Therefore, the potential of extracts of L. camara and essential oils (EOs) for the production and protection of the tomato variety “Roma VF” was studied under field conditions in Ibadan, Nigeria. Fresh L. camara leaves were collected from plants growing on the University of Ibadan campus, Oyo State and identified at the Herbarium section, Department of Botany, University of Ibadan (UIH 23103). The leaves were air-dried, pulverized and subjected to hydrodistillation for EO extraction. The methanol and aqueous extracts of L. camara were obtained by maceration in methanol and water, respectively. An in vivo pesticidal bioassay was conducted to determine the biocidal potential of L. camara leaves using aqueous extract, methanolic extract, EO and water (as control) via two modes of application (spraying and drenching). The data were analysed using ANOVA and DMRT. The EO treatment gave the best control of the pests and resulted in higher fruit yield (1821 g · plant‒1) compared to the control (512.5 g · plant‒1). Thus, the EO from L. camara leaves demonstrated promising biocidal potential and can be effectively used to protect tomatoes against pests for increased fruit yield.
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Authors and Affiliations

Ayopo Anuoluwa Sotade
1
ORCID: ORCID
Olapeju Oluyemisi Aiyelaagbe
2
ORCID: ORCID
Adefoyeke Olufunmilayo Aduramigba-Modupe
3
ORCID: ORCID
Isaac Oreoluwa Aiyelaagbe
4
ORCID: ORCID

  1. Department of Chemistry, University of Ibadan, Ibadan, Nigeria, F106 Laboratory, Natural Products Laboratory, Ibadan, Nigeria
  2. Department of Chemistry, University of Ibadan, Ibadan. Nigeria
  3. Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria
  4. Horticulture (Fruit Science and Cropping Systems), Federal University of Agriculture, Department of Horticulture, Abeokuta, Nigeria
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Abstract

The effects of four extracts of Hortia oreadica Groppo (Rutaceae) on the biological parameters, nutritional indices, and activity of digestive enzymes of Anticarsia gemmatalis Hübner [1818] (Erebidae) were evaluated. Newly hatched caterpillars were subjected to extracts from H. oreadica extracted with hexane (HE), dichloromethane (DE), dichloromethane fractionated with ethyl acetate (DEAE) and dichloromethane fractionated with dichloromethane (DDE) at concentrations 0, 100, 500 and 1000 μg ꞏ ml–1. The results showed that treatments significantly increased the cumulative mortality rate of A. gemmatalis and that HE 100 μg ꞏ ml–1 was the most effective, causing 92% of insects’ deaths. The period of larval development was significantly shorter with treatments HE 100 and 500 μg ꞏ ml–1 and longer with DEAE (all concentrations). The pupal weight was lower in the treatments HE 1000 μg ꞏ ml–1 and in all doses tested of the DEAE treatment. The morphological integrity of pupae and adults was not affected. The lowest values of relative food consumption rate, relative metabolic rate, and relative growth rate were observed in the HE 100 μg ꞏ ml–1 treatment. In most treatments, there was a reduction in the activity of total proteases, serine and cysteine proteases, and amylase. The results suggest that the HE extract of H. oreadica is the most efficient in controlling A. gemmatalis, due to the presence of deterrent metabolites that affect the insect’s digestion and nutrition and consequently promote adverse effects on its development and mortality.
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Authors and Affiliations

Liliane Evangelista Visotto
1
ORCID: ORCID
Vinícius Guimarães Nasser
2
ORCID: ORCID
Vanessa Gisele Pasqualotto Severino
3
ORCID: ORCID
Márcio Santos Soares
4
Vinícius Ribeiro Faria
2
ORCID: ORCID
Pedro Ivo Vieira Good God
2
ORCID: ORCID

  1. Institute of Biological and Health Sciences, Universidade Federal de Viçosa Rio Paranaíba, Brazil
  2. Institute of Agricultural Sciences, Universidade Federal de Viçosa Rio Paranaíba, Brazil
  3. Chemistry Institute, Universidade Federal de Goiás, Avenida Esperança, Goiania, Brazil
  4. Institute of Exact and Technological Sciences, Universidade Federal de Viçosa Rio Paranaíba, Brazil
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Abstract

Beauveria bassiana has proven to be an efficient crop colonizer due to its highly entomopathogenic activity against a wide range of sap-sucking pests. In this work, two different application methods of B. bassiana (Bals.-Criv.) strain PPRI 5339 were studied over two consecutive years: (A) seed coating on cotton and (B) trunk inoculation to kiwi trees. Untreated plants served as controls for both methods. The sucking insects, Aphis gossypii (Glover) on cotton plants, and Halyomorpha halys (Stål) on kiwi trees, were counted, and the total chlorophyll content and leaf area were measured as indicators of plant performance. The presence of B. bassiana slightly reduced the population of sucking pests but also enhanced the total chlorophyll content and the leaf area in both crops. These results demonstrate the dual role of strain PPRI 5339 as both a biocontrol agent and a growth promoter, highlighting its potential as an environmentally friendly alternative for managing sap-sucking pests that severely affect agricultural production in Greece
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Authors and Affiliations

Vasileios Papantzikos
1
Spiridon Mantzoukas
2
George Patakioutas
1

  1. Department of Agriculture, University of Loannina, Arta, Greece
  2. Institute of Mediterranean Forest Ecosystems, Terma Alkmanos Ilisia, Athina, Greece
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Abstract

During a survey conducted in Hamedan Province, Iran, Thompson seedless grapevine (Vitis vinifera) bunches were observed exhibiting symptoms of brown ripe rot. To identify the causal agent, samples were collected, and fragments from the infected tissues were cultured on PCA and PDA media. Morphological studies and sequencing analyses of the ITS region were performed to identify the emerging fungus. To confirm the pathogenicity of the fungal isolate, Koch’s postulates were applied. Based on morphological assessments, pathogenicity test, and the ITS sequences, the causal agent was identified as Arthrinium sacchari (Apiosporaceae, Sordariomycetes). This study provides new information on ripe rot caused by A. sacchari in grapevine plants.
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Authors and Affiliations

Zahra Vesaltalab
1
Mansour Gholami
1
Jalal Soltani
2
ORCID: ORCID

  1. Department of Horticulture, Agriculture Faculty, Bu-Ali Sina University, Hamedan, Iran
  2. Phytopathology Section, Plant Protection Department, Bu-Ali Sina University, Hamedan, Iran

Instructions for authors

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

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Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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