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Immuno-affinity chromatography for purification of IgG from hyper-immune sera raised against 146S fraction of Foot and Mouth Disease Virus for diagnostic purposes

A. Munir A.A. Anjum I. Altaf A.R. Awan
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 3 | 393–399 | DOI: 10.24425/pjvs.2023.145045
Słowa kluczowe enzyme linked immuno-sorbent assay (ELISA) Foot and Mouth disease virus (FMDV) hyper-immune sera (HIS) immunoglobulin G (IgG) immuno-affinity chromatography (IAC) protein-A size exclusion chromatography (SEC)
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Abstrakt

Immunoaffinity chromatography (IAC) is a fundamental isolation and purification tool which is incorporated in a substantial range of therapeutic and diagnostic applications. This study has reappraised the usefulness of immunoaffinity chromatography for the purification of polyclonal antibodies. Protein A based IAC is a convenient and reliable method for purification of IgG, from hyperimmunesera (HIS) raised in experimental animals such as rabbits, guinea pigs and mice to be utilized in pharmaceutics and diagnostics. The 146S fraction of Foot and Mouth Disease virus (FMDV) TCID50=10 5.6 was cultured on a baby hamster kidney cell line 21 (BHK-21), concentrated using salt precipitation method using PEG 6000, purified by size exclusion chromatography (SEC) using Sepharose-30 at 254nm absorbance. Purification of 146S FMDV was analyzed using 12% SDS-PAGE which provided two bands of light and heavy chains. The alum-based vaccine, consisting of ≥10μg of 146S FMDV, was applied in 10 male rabbits and 10 male guinea pigs and two animals of each group were taken as a negative control. The titer of serum was calculated using virus neutralization test. A Protein-A kit (Thermo scientific- 44667, 0528.2) was used to purify HIS raised against 146S FMDV and validated using 12% SDS PAGE in reducing condition. The data demonstrate that protein-A affinity chromatography is an efficient tool for the purification of antibodies from hyper-immune sera raised against 146S FMDV and can be used for the production of diagnostic kits e.g. Enzyme linked immuno-sorbent assay (ELISA) and radioimmunoassay.
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Bibliografia


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Autorzy i Afiliacje

A. Munir
1
A.A. Anjum
1
I. Altaf
2
A.R. Awan
3
  1. Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Outfall road, Lahore, Pakistan
  2. Quality Operations Laboratory, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Outfall road, Lahore, Pakistan
  3. Department of Biochemistry and Biotechnology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Outfall road, Lahore, Pakistan

Groundwater quality and hydrogeological characteristics of Malacca state in Malaysia

Sharif Moniruzzaman Shirazi Md. Ibrahim Adham Noorul Hassan Zardari Zubaidah Ismail Hosen Md Imran Munir Ahmed Mangrio
Journal of Water and Land Development | 2015 | No 24
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Autorzy i Afiliacje

Sharif Moniruzzaman Shirazi
Md. Ibrahim Adham
Noorul Hassan Zardari
Zubaidah Ismail
Hosen Md Imran
Munir Ahmed Mangrio

Impact of tractor wheels on physical properties of different soil types and the irrigation efficiency of the furrow irrigation method

Rahim Bux Vistro Mashooque Ali Talpur Irfan Ahmed Shaikh Munir Ahmed Mangrio
Journal of Water and Land Development | 2022 | No 52 | 166-171 | DOI: 10.24425/jwld.2022.140386
Słowa kluczowe furrow/ridge storage efficiency irrigation method soil physical properties tractor wheel trafficking water use efficiency
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Abstrakt

In furrow irrigation, the maximum lateral movement of water in ridges is more desirable than the vertical downward movement. This can be achieved by compacting the furrows. Thus, the study examines the impact on furrow soil compaction by tractor wheel trafficking during mechanical operations in the different soil types. In this experiment, the three-wheel tractor compaction includes: 1) control (no soil compaction), 2) compaction through 3-wheel tractor passes, and 3) compaction through 6-wheel passes under three different soil textural classes such as: clay loam, silty clay loam and silty loam soils. The impact of various treatments on clay loam, silty clay loam, and silty loam under 3- and 6-wheel passes showed increased bulk density (7–12%), field capacity (9–19%), ridge storage efficiency (35–38%), water use efficiency (16–20.5%) and decreased soil porosity (7–16%), infiltration (8–20%), and furrow storage efficiency (28–41%) over the control. This study shows comparable results of 6-passes with other studies in which more than 6-passes were used to compact the soil. This study suggested that farmers can maximise water use efficiency by compacting their furrows using 6-passes tractor trafficking.
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Bibliografia

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Autorzy i Afiliacje

Rahim Bux Vistro
1
Mashooque Ali Talpur
1
Irfan Ahmed Shaikh
1
Munir Ahmed Mangrio
1
  1. Sindh Agriculture University, Faculty of Agricultural Engineering, Tandojam, Hyderabad, 70060, Sindh, Pakistan

Soil moisture distribution pattern of surface drip irrigated mango

Shoukat Ali Soomro Muhammad Saffar Mirjat Munir Ahmed Mangrio Mashooque Ali Talpur
Journal of Water and Land Development | 2022 | No 53 | 158-163 | DOI: 10.24425/jwld.2022.140792
Słowa kluczowe double periphery drip irrigation mango orchard moisture content flow duration
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Abstrakt

At present, Pakistan has been facing acute shortage of irrigation water and farmers have been using conventional irrigation methods for orchards, such as flood and basin irrigation, thus wasting huge amount of fresh water. Therefore, it is necessary to find efficient irrigation methods to cope with this major burning issue. The micro drip irrigation method is considered efficient but in the case of mango orchards there is a problem of irrigation frequency, number of emitters, and duration of flow from emitters to meet water demand. Considering the above, an experiment was conducted in the experimental field of the Sindh Agriculture University, Tandojam, by installing the drip system with two circular peripheries of lateral lines in clay loam soil covering the entire canopy of a mature mango tree. The radius of the first and second periphery around the tree trunk was 100 cm and 150 cm, respectively. Four emitters with 4 dm3∙h –1 discharge of individual dipper were fixed in each periphery. Emitters were tested for six different irrigation times, i.e. 1, 2, 3, 4, 5 and 6 h, to observe the moisture distribution pattern. Hydraulic characteristics, such as density, field capacity, porosity, infiltration rate, available water and permanent wilting point (PWP), were determined using standard methods (1.4 g∙cm –3, 33%, 49%, 8 mm∙h –1, 12.41% and 20% respectively). The texture class of the soil profile was determined as clay loam at the soil depth 0–120 cm. Fifty soil samples were collected at 0–10, 10– 30, 30–60, 60–90, and 90–120 cm depths and at 0–20, 20–40, 40–60, 60–80 and 80–100 cm distances on two opposite sides of emitters. The emitters provided sufficient moisture up to field capacity in clay loam soil with flow duration of 4 h. The maximum moisture distribution efficiency was 77.89% with flow duration of 4 h at vertical depth of 0–120 cm and 0–100 cm distance horizontally among four emitters as compared to 1, 2, 3 h flow duration which under irrigated the canopy area and 5, 6 h flow duration which excessively irrigated the canopy area of the mango tree. The water demand of the mango tree was met by 4 h flow duration which provided adequate moisture to the entire canopy up to 120 cm depth in the root zone and water saving was calculated as 15.91% under the installed drip irrigation system as compared with the conventional (basin) irrigation method.
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Autorzy i Afiliacje

Shoukat Ali Soomro
1
ORCID: ORCID
Muhammad Saffar Mirjat
1
Munir Ahmed Mangrio
1
Mashooque Ali Talpur
1
  1. Sindh Agriculture University, Tandojam, Faculty of Agricultural Engineering, 70060, Hyderabad, Pakistan

Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress

Nur Athirah Yusof Clemente Michael Vui Ling Wong Abdul Munir Abdul Murad Farah Diba Abu Bakar Nor Muhammad Mahadi Ahmad Yamin Abdul Rahman Nursyafiqi Zainuddin Mohd Nazalan Mohd Najimudin
Polish Polar Research | 2019 | vol. 40 | No 3 | 273-292 | DOI: 10.24425/ppr.2019.129674
Słowa kluczowe Antarctic psychrophile molecular chaperone heat-stress transcriptome
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Abstrakt

Microbes living in the polar regions have some common and unique strategies to respond to thermal stress. Nevertheless, the amount of information available, especially at the molecular level is lacking for some organisms such as Antarctic psychrophilic yeast. For instance, it is not known whether molecular chaperones in Antarctic yeasts play similar roles to those from mesophilic yeasts when they are exposed to heat stress. Therefore, this project aimed to determine the gene expression patterns and roles of molecular chaperones in Antarctic psychrophilic Glaciozyma antarctica PI12 that was exposed to heat stress. G. antarctica PI12 was grown at its optimal growth temperature of 12ºC and later exposed to heat stresses at 16ºC and 20ºC for 6 hours. Transcriptomes of those cells were extracted, sequenced and analyzed. Thirty-three molecular chaperone genes demonstrated differential expression of which 23 were up-regulated while 10 were down-regulated. Functions of up-regulated molecular chaperone genes were related to protein binding, response to a stimulus, chaperone binding, cellular response to stress, oxidation, and reduction, ATP binding, DNA-damage response and regulation for cellular protein metabolic process. On the other hand, functions of down-regulated molecular chaperone genes were related to chaperone-mediated protein complex assembly, transcription, cellular macromolecule metabolic process, regulation of cell growth and ribosome biogenesis. The findings provided information on how molecular chaperones work together in a complex network to protect the cells under heat stress. It also highlights the evolutionary conserved protective role of molecular chaperones in psychrophilic yeast, G. antarctica, and mesophilic yeast, Saccharomyces cerevisiae.

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Autorzy i Afiliacje

Nur Athirah Yusof
Clemente Michael Vui Ling Wong
ORCID: ORCID
Abdul Munir Abdul Murad
Farah Diba Abu Bakar
Nor Muhammad Mahadi
Ahmad Yamin Abdul Rahman
Nursyafiqi Zainuddin
Mohd Nazalan Mohd Najimudin

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