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Title: Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms

Authors:

Podwin, Agnieszka ; Janisz, Tymon ; Patejuk, Katarzyna ; Szyszka, Piotr ; Walczak, Rafał ; Dziuban, Jan

Date:

9.02.2021

Type:

Article

Affiliation:

Podwin, Agnieszka : Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, Poland ; Janisz, Tymon : Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, Poland ; Patejuk, Katarzyna : Wrocław University of Environmental and Life Sciences, Department of Plant Protection, Grunwaldzki Sq. 24a, 50-363 Wroclaw, Poland ; Szyszka, Piotr : Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, Poland ; Walczak, Rafał : Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, Poland ; Dziuban, Jan : Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, Poland

Coverage:

e136212

Abstract:

This paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection, by means of modern, miniaturized, portable and dependable microfluidics instrumentation. The authors have presented herein long-term, successful cultivation of different fungi representatives (with emphasis put on Cladosporium macrocarpum) in specially fabricated all-glass LOCs. Notable differences were noted in the development of these creatures on polymer, polydimethylosiloxane (PDMS) cultivation substrates, revealing the uncommon morphological character of the fungi mycelium. The utility of all-glass LOCs was verified for other fungi representatives as well –  Fusarium culmorum and Pencilium expansum, showing technical correspondence and biocompatibility of the devices. On that basis, other future applications of the solution are possible, covering, e.g. investigation of additional, environmentally relevant fungi species. Further development of the LOC instrumentation is also taken into consideration, which could be used for cultivation of other soil organisms and study of their mutual relationships within the integrated microfluidic device.

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Identifier:

oai:journals.pan.pl:119101 ; DOI: 10.24425/bpasts.2020.136212

Subject and keywords:

microfluidics lab-chip biomaterials cell cultivation mycology

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Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms Dec 16, 2025

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Authors and Affiliations

Mahima Ganguly
1
Jithu Valiamparampil
1
Divyashree Somashekara
2
Lavanya Mulky
1
  1. Department of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, 576104, India
  2. Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, 576104, India

Intelligent Polymers

Agnieszka Kowalczuk Alicja Utrata-Wesołek Barbara Trzebicka
ACADEMIA. The magazine of the Polish Academy of Sciences | 2024 | No 4(84) Health | 40-43 | DOI: 10.24425/academiaPAS.2024.152929
Keywords biomaterials polymers burns
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Abstract

An interdisciplinary team of Polish scientists has developed innovative “smart” biomaterials to aid in the treatment of painful burn wounds.
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Authors and Affiliations

Agnieszka Kowalczuk
1
Alicja Utrata-Wesołek
1
Barbara Trzebicka
1
  1. Laboratory of Nano- and Microstructural Materials, Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze

Investigations of modular microfluidic geometries for passive manipulations on droplets

D. Zaremba S. Blonski M. Jachimek M.J. Marijnissen S. Jakiela P.M. Korczyk
Bulletin of the Polish Academy of Sciences Technical Sciences | 2018 | 66 | No 2 (Special Section on Nano- and Micromechanics) | 139-149
Keywords microfluidics two-phase flows droplets
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Authors and Affiliations

D. Zaremba
S. Blonski
M. Jachimek
M.J. Marijnissen
S. Jakiela
P.M. Korczyk

Numerical simulation of multiple species detection using hydrodynamic/electrokinetic focusing

J. Hahm A. Beskok
Bulletin of the Polish Academy of Sciences Technical Sciences | 2005 | vol. 53 | No 4 | 325-334
Keywords Species detection electrophoresis hydrodynamic/electrokinetic focusing microfluidics
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Abstract

In this paper we present the numerical simulation-based design of a new microfluidic device concept for electrophoretic mobility and (relative) concentration measurements of dilute mixtures. The device enables stationary focusing points for each species, where the locally applied pressure driven flow (PDF) counter balances the species’ electrokinetic velocity. The axial location of the focusing point, along with the PDF flowrate and applied electric field reveals the electrokinetic mobility of each species. Simultaneous measurement of the electroosmotic mobility of an electrically neutral specie can be utilized to calculate the electrophoretic mobility of charged species. The proposed device utilizes constant sample feeding, and results in time-steady measurements. Hence, the results are independent of the initial sample distribution and flow dynamics. In addition, the results are insensitive to the species diffusion for large Peclet number flows (Pe > 400), enabling relative concentration measurement of each specie in the dilute mixture.
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Authors and Affiliations

J. Hahm
A. Beskok

Study on the Effect of in Situ Surface Treatments of Medical Microfluidic Systems Obtained Through Additive Manufacturing

A. Csapai D.-A. Țoc V. Pașcalău V. Toșa D. Opruța F. Popa C. Popa
Archives of Metallurgy and Materials | 2022 | vol. 67 | No 3 | 1071-1078 | DOI: 10.24425/amm.2022.139705
Keywords additive manufacturing microfluidic systems surface roughness biofilm growth laminar flow
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Abstract

This paper presents a comparative study on the effects of the in-situ surface modifications performed on “H” type microfluidic systems obtained via additive manufacturing. The microsystem was printed using a polylactic acid filament on an Ender-5 Pro printer. The surface modification of the main channel was done using chloroform by two different methods: vapor smoothing and flushing. The obtained surface roughness was studied using an optical microscope and the ImageJ software, as well as scanning electron microscopy. The effect of the channel surface treatment upon the characteristics of the fluid flow was assessed. The microfluidic systems were used for the dynamic study of biofilm growth of Candida albicans (ATCC 10231). The influence of the surface roughness of the main channel on the formation and growth of the biofilm was studied using quantitative methods, scanning electron microscopy imaging as well as optical coherence tomography.
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Authors and Affiliations

A. Csapai
1
ORCID: ORCID
D.-A. Țoc
2
ORCID: ORCID
V. Pașcalău
1
ORCID: ORCID
V. Toșa
1
ORCID: ORCID
D. Opruța
3
ORCID: ORCID
F. Popa
1
ORCID: ORCID
C. Popa
ORCID: ORCID
  1. Materials Science and Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania
  2. Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400000 Cluj-Napoca, Romania
  3. Thermal Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania

The Influence of Plastic Forming Processes on the Dissolution Rate of Biocompatible Mg Alloys

S. Boczkal M. Karaś P. Korczak D. Kapinos P. Koprowski W. Szymański S. Wroński
Archives of Metallurgy and Materials | 2019 | vol. 64 | No 3 | 997-1004 | DOI: 10.24425/amm.2019.129486
Keywords MgLiCaZn alloys biomaterials structure and properties immersion test
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Abstract

The effect of plastic deformation process on the dissolution rate of biocompatible Mg alloys was investigated. Two biocompatible MgLi1Ca0,2Zn1 and MgLi1Ca1Zn1 alloys were selected for the study. The alloys were deformed on a 100T press at a temperature of 350°C by conventional extrusion and by the equal channel angular extrusion process (ECAE). The grain size analysis showed a high degree of the grain refinement from approximately 110 mm in the initial state to 2.8 mm after the 3rd pass of the ECAE process. Compared to as-cast state, the degree of strengthening has increased after plastic forming. The results of biodegradation tests have shown a significant increase in corrosion rate after both conventional extrusion and ECAE, although after subsequent ECAE passes, this rate was observed to slightly decrease in the MgLi1Ca1Zn1 alloy. Based on the results of macro- and microstructure examinations, the corrosion progress in samples after the extrusion process was described.
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Authors and Affiliations

S. Boczkal
M. Karaś
P. Korczak
D. Kapinos
P. Koprowski
W. Szymański
S. Wroński
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