Between 2004 and 2017, multiple studies on the herbicide resistance of weeds were conducted by the Institute of Plant Protection – National Research Institute in Poland. Weed seeds, collected from fields located in various regions of Poland where herbicide use was ineffective, were used in studies conducted under greenhouse conditions. A total of 261 loose silky bent (Apera spica-venti L.) samples were found to be herbicide resistant, which translates to 52.4% of the fields under study. Nearly 50% of the analyzed samples exhibited resistance to sulfonylurea herbicides. Resistance to acetyl CoA carboxylase (ACCase) inhibitors was found in 18 fields, whereas resistance to photosystem II (PSII) inhibitors (isoproturon) was found in 12 fields. Herbicide resistance of blackgrass (Alopecurus myosuroides Huds.) occurred in 26 of the fields under study. In addition, resistance of wild oat (Avena fatua L.) to acetyl CoA carboxylase inhibitors occurred in 10 spring cereal crops. In the case of winter wheat, resistance of cornflower (Centaurea cyanus L.) to tribenuron-methyl occurred in 23 fields. Scentless chamomile (Matricaria inodora L.) and field poppy (Papaver rhoeas L.) were resistant to tribenuron-methyl in four and three fields, respectively, of winter wheat. In the case of sugar beet, three biotypes of fat hen (Chenopodium album L.) and two biotypes of redroot amaranth (Amaranthus retroflexus L.) were resistant to metamitron. Horseweed (Conyza canadensis L.), which grows on railway tracks, exhibited resistance to glyphosate. This paper reviews all studies conducted in Poland on weed resistance. Based on the results, maps of weed resistance in Poland were created.
Fusarium crown rot (FCR), caused by Fusarium culmorum (Wm.G.Sm) Sacc., is an important disease of wheat both in Iraq and other regions of wheat production worldwide. Changes in environmental conditions and cultural practices such as crop rotation generate stress on pathogen populations leading to the evolution of new strains that can tolerate more stressful environments. This study aimed to investigate the genetic diversity among isolates of F. culmorum in Iraq. Twenty-nine samples were collected from different regions of wheat cultivation in Iraq to investigate the pathogenicity and genetic diversity of F. culmorum using the repetitive extragenic palindromic (REP-PCR) technique. Among the 29 isolates of F. culmorum examined for pathogenicity, 96% were pathogenic to wheat at the seedling stage. The most aggressive isolate, from Baghdad, was IF 0021 at 0.890 on the FCR severity index. Three primer sets were used to assess the genotypic diversity via REP, ERIC and BOX elements. The amplicon sizes ranged from 200–800 bp for BOX-ERIC2, 110–1100 bp for ERIC-ERIC2 and 200–1300 bp for REP. A total of 410 markers were polymorphic, including 106 for BOX, 175 for ERIC and 129 for the REP. Genetic similarity was calculated by comparing markers according to minimum variance (Squared Euclidean). Clustering analysis generated two major groups, group 1 with two subgroups 1a and 1b with 5 and 12 isolates, respectively, and group 2 with two subgroups 2a and 2b with 3 and 9 isolates, respectively. This is the first study in this field that has been reported in Iraq.
Hass avocado cultivation in Colombia has grown rapidly in area in recent years. It is being planted in marginal areas, which leads to low yields, and in many cases is related to diseases. Ecological niche modeling (ENM) can offer a view of the potential geographic and environmental distribution of diseases, and thus identify areas with suitable or unsuitable conditions for their development. The aim of the study was to assess current and potential distribution of the major diseases on Hass avocado in Colombia. Areas planted with Hass avocado in Antioquia, Colombia were sampled for diseases including the following pathogens: Phytophthora cinnamomi, Verticillium sp., Lasiodiplodia theobromae, Phytophthora palmivora, Colletotrichum gloeosporioides sensu lato, Pestalotia sp., and Capnodium sp., and one disorder hypoxia-anoxia. These pathogens were selected based on their relevance (incidence-severity) and capacity to cause damage in different tissues of avocado plants. Severity and incidence of each disease were related to environmental information from vegetation indices and topographic variables using maximum entropy modeling approaches (MaxEnt). Models were calibrated only across areas sampled, and then transferred more broadly to areas currently planted, and to potential zones for planting. Combinations of best performance and low omission rates were the basis for model selection. Results show that Hass avocado has been planted in areas highly conducive for many pathogens, particularly for Phytophthora cinnamomi and hypoxia-anoxia disorder. Ecological niche modeling approaches offer an alternative toolset for planning and making assessments that can be incorporated into disease management plans.
Development and demography of Adalia decempunctata L. were studied under laboratory conditions at seven constant temperatures (12, 16, 20, 24, 28, 32 and 36°C). First instar larvae failed to develop to second instar at 12°С and no development occurred at 36°C. The total developmental time varied from 47.92 days at 16°C to 15.94 days at 28°C and increased at 32°C. The lower temperature thresholds of 11.05 and 9.90°C, and thermal constants of 290.84 day-degree and 326.34 day-degree were estimated by traditional and Ikemoto-Takai linear models, respectively. The lower temperature threshold (Tmin) values estimated by Analytis, Briere-1, Briere-2 and Lactin-2 for total immature stages were 11.99, 12.24, 10.30 and 10.8°C, respectively. The estimated fastest developmental temperatures (Tfast) by the Analytis, Briere-1, Briere-2 and Lactin-2 for overall immature stages development of A. decempunctata were 31.5, 31.1, 30.7 and 31.7°C, respectively. Analytis, Briere-1, Briere-2 and Lactin-2 measured the upper temperature threshold (Tmax) at 33.14, 36.65, 32.75 and 32.61°C. The age-stage specific survival rate (sxj) curves clearly depicted the highest and lowest survival rates at 16 and 32°C for males and females. The age-specific fecundity (mx) curves revealed higher fecundity rate when fed A. gossypii at 24 and 28°C. The highest and lowest values of intrinsic rate of increase (r) were observed at 28 and 16°C (0.1945 d–1 and 0.0592 d–1, respectively). Also, the trend of changes in the finite rate of increase (λ) was analogous with intrinsic rate of increase. The longest and shortest mean generation time (T) was observed at 16 and 28°C, respectively and the highest net reproductive rates (R0) was estimated at 24 and 28°C. According to the results, the most suitable temperature seems to be 28°C due to the shortest developmental time, highest survival rate, and highest intrinsic rate of increase.
Spectroscopy has become one of the most used non-invasive methods to detect plant diseases before symptoms are visible. In this study it was possible to characterize the spectral variation in leaves of Solanum lycopersicum L. infected with Fusarium oxysporum during the incubation period. It was also possible to identify the relevant specific wavelengths in the range of 380–1000 nm that can be used as spectral signatures for the detection and discrimination of vascular wilt in S. lycopersicum. It was observed that inoculated tomato plants increased their reflectance in the visible range (Vis) and decreased slowly in the near infrared range (NIR) measured during incubation, showing marked differences with plants subjected to water stress in the Vis/NIR. Additionally, three ranges were found in the spectrum related to infection by F. oxysporum (510–520 nm, 650–670 nm, 700–750 nm). Linear discriminant models on spectral reflectance data were able to differentiate between tomato varieties inoculated with F. oxysporum from healthy ones with accuracies higher than 70% 9 days after inoculation. The results showed the potential of reflectance spectroscopy to discriminate plants inoculated with F. oxysporum from healthy ones as well as those subjected to water stress in the incubation period of the disease.
Clethodim herbicide (Cle) and three Trichoderma strains (Tri) were applied either alone or in combination (Cle + Tri) for controlling weeds, root knot nematodes (Meloidogyne arenaria) and Rhizoctonia root rot disease (Rhizoctonia solani) as well as for evaluating their effects on total microbial count in the rhizosphere and the number of Rhizobium nodules on roots in two faba bean cultivars cultivated in naturally heavily infested fields. The evaluated characters were very similar for the two tested cultivars (Nubariya 1 and Sakha 3). Treatment with Cle alone highly reduced the fresh and dry matter of tested weeds (Amaranthus viridis, Cynodon dactylon and Cenchrus ciliaris), followed by Cle + Tri and Tri alone. Cle + Tri highly reduced nematode parameters viz. numbers of J2 in soil or roots, females, eggs, galls and egg-masses when compared with each treatment alone. Tri alone caused a great decrease in Rhizoctonia root rot infection, followed by Cle + Tri and Cle alone. Total microbial count and Rhizobium nodules were affected only with Cle treatment. Plant growth parameters (shoot length, shoot fresh and dry weight and numbers of branches and leaves) and yield parameters (fresh pod and dry weight, seed number per pod, seed weight and ash pod weight of plant) were greatly improved for Cle + Tri treatments when compared with either Tri or Cle alone.
The objective of this work was to generate a series of equations to describe the voltinism of Lobesia botrana in the quarantine area of the main winemaking area of Argentina, Mendoza. To do this we considered an average climate scenario and extrapolated these equations to other winegrowing areas at risk of being invaded. A grid of 4 km2 was used to generate statistics on L. botrana captures and the mean temperature accumulation for the pixel. Four sets of logistic regression were constructed using the percentage of accumulated trap catches/grid/week and the degree-day accumulation above 7°C, from 1st July. By means of a habitat model, an extrapolation of the phenological model generated to other Argentine winemaking areas was evaluated. According to our results, it can be expected that 50% of male adult emergence for the first flight occurs at 248.79 ± 4 degree-days (DD), in the second flight at 860.18 ± 4.1 DD, while in the third and the fourth flights, 1671.34 ± 5.8 DD and 2335.64 ± 4.3 DD, respectively. Subsequent climatic comparison determined that climatic conditions of uncolonized areas of Cuyo Region have a similar suitability index to the quarantine area used to adjust the phenological model. The upper valley of Río Negro and Neuquén are environmentally similar. Valleys of the northwestern region of Argentina showed lower average suitability index and greater variability among SI estimated by the algorithm considered. The combination of two models for the estimation of adult emergence time and potential distribution, can provide greater certainties in decision-making and risk assessment of invasive species.
This weed management investigation was carried out at the Zonal Agricultural Research Station (ZARS), Bangalore, during the summers of 2017 and 2018 to standardize agrotechniques for weed management of rice grown under aerobic conditions. The experiment was laid out in a randomized complete block design with eleven treatments replicated thrice. It consisted of two pre-emergence herbicides and one early post-emergence herbicide, the stale seedbed technique, mulching, hand weeding and intercultivation which was compared to the weedy check. The results showed that pyrazosulfuron ethyl 10% wettable powder (WP) at 35 active ingredient (a.i.) g ⋅ ha–1 as PE fb bispyribac sodium 10% SC at 30 ml ⋅ ha–1 a.i. as an early post-emergence herbicide performed better in terms of rice grain and straw yield (5,800 and 9,786 kg ⋅ ha–1, respectively), plant height (58.42 cm), rice total dry matter production (149.84 g ⋅ plant–1), productive tillers ⋅ hill–1 (40.32), panicle length (24.53 cm), 1000 grain weight (25.35 g), net returns (Rs. 62424), higher B : C ratio (2.59) and lower total weed density, weed dry weight at different stages of rice and weed index (3.80%) as well as higher weed control efficiency (90.52%). This practice could be recommended to farmers growing aerobic rice under these climatic conditions.
The current research aimed to use non traditional methods to control some stored grain insects. The effects of 180 millitesla (mT) magnetic field (MF) for six different exposure periods (3 min, 30 min, 1 h, 12 h, 24 h and 48 h) on mortality (%) of two stored grain insects, Tribolium casteneum adults and Trogoderma granarium larvae, reduction in F1-progeny (%), seeds germination (%) and seed components (%) after 8 months storage period were studied under laboratory conditions. According to results, the mortality (%) of tested insects increased with increasing of MF time exposure. Trogoderma granarium was more resistant than T. casteneum in which mortality reached 56 and 75%, respectively 14 days after from exposure period. Without any negative effect on seeds germination (%) the MF was very effective in protecting stored wheat from insect infestation up to 8 months compared to non-magnetic seeds which became infested after 3 months of storage. Furthermore, the germination (%) was accelerated by 6 h compared to non-magnetic seeds. The MF level caused a slight increase in the percent of total carbohydrate, crude protein and ash while slightly decrease the percent of moisture, total fats and crude fiber.
Fusarium head blight (FHB) is one of the most important diseases that occurs in cereal regions worldwide and causes serious economic damage. This disease can be caused by several Fusarium species with Fusarium graminearum sensu stricto being the most common pathogen isolated from several crops. The aim of this study was to report the occurrence of F. graminearum sensu stricto on rye grains collected from field samples in Argentina and to determine the potential ability to produce deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEA). Based on morphological characteristics, the isolate was identified as F. graminearum sensu stricto. To confirm molecularly, portions of the RED and TRI genes were sequenced and showed 99% similarity with the F. graminearum sensu stricto sequences available in the NCBI database. The potential to produce DON, 15-acetyldeoxynivalenol (15-ADON) and ZEA was determined. Moreover, Koch´s postulates were carried out. To our knowledge, this is the first report of F. graminearum sensu stricto associated with rye kernels in Argentina.