Enzymatic antioxidant defense in isolated rat hepatocytes exposed to cadmium

Journal title

Polish Journal of Veterinary Sciences




No 4


Divisions of PAS

Nauki Biologiczne i Rolnicze


Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn




DOI: 10.2478/v10181-010-0002-7 ; ISSN 1505-1773


Polish Journal of Veterinary Sciences; 2010; No 4


Beauchamp C. (1971), Superoxide dismutase: improved assays and an assay applicable to acrylamide gels, Anal Biochem, 44, 276. ; Bojakowska K (<b>2006</b>) Evaluation of cytotoxicity of cadmium compounds: in vitro studies on rat and equine isolated hepatocytes. Doctoral thesis, Warsaw, Warsaw University of Life Sciences, Poland. ; Borzęcka K. (2004), Evaluation of cadmium cytotoxicity in isolated hepatocytes: effect of plating time, medium composition and cadmium chemical compounds, null, 32. ; Bradford M. (1976), A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding, Anal Biochem, 72, 248. ; Brzóska M. (1998), The influence of calcium content in the diet on accumulation and toxicity of cadmium in the organism, Arch Toxicol, 72, 63. ; Casalino E. (2002), Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium, Toxicology, 179, 37. ; European Food Safety Authority (<b>2009</b>) Cadmium in food. Scientific Opinion of the panel on contaminants in the Food Chain. EFSA J 980: 1-139. ; Ellman G. (1959), Tissue sulfhydryl groups, Arch Biochem Biophys, 82, 70. ; Fariss M. (1991), Cadmium toxicity: unique cytoprotective properties of alpha tocopheryl succinate in hepatocytes, Toxicology, 69, 63. ; Golberg D. (1983), Glutathione reductase. Methods Enzymatic Analysis, Verlag Chemie, Weinheim, 3, 258. ; Góth L. (1999), A simple method for determination of serum catalase activity and revision of reference range, Clin Chim Acta, 196, 143. ; Habig W. (1974), Glutathione S-transferase: The first step in mercapturic acid formation, J Biol Chem, 249, 7130. ; Hayes J. (1999), Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stress, Free Radic Res, 31, 273. ; International Agency for Research on Cancer (IARC) (<b>1993</b>) Beryllium, cadmium, mercury, and exposure in the glass manufacturing industry. Monographs on the evaluation of the carcinogenic risks to humans. IARC Scientific Publications, Lyon, 58, pp 119-237. ; Invitox Protocol Number 20. Isolation of Rat Hepatocytes. Invitox, Protocol ERGATT/FRAME Data bank of In Vitro Techniques in Toxicology (<b>1991</b>) ; Järup L. (1998), Health effects of cadmium exposure: a review of the literature and a risk estimate, Scand J Work Environ Health, 24, 1. ; Jurczuk M. (2004), Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol, Food Chem Toxicol, 42, 429. ; Kehrer J. (1993), Free radicals as mediators of tissue injury and disease, Crit Rev Toxicol, 23, 21. ; Kołacz R. (1996), Bio-accumulation of Cd, Pb and Hg in animal tissues, Veterinary Medicine (Polish), 52, 686. ; Oberley L. (1984), Assay of superoxide dismutase activity in tumor tissue, Methods Enzymol, 105, 457. ; Ohkawa H. (1979), Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal Biochem, 95, 351. ; Paglia D. (1967), Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase, J Lab Clin Med, 70, 158. ; Pastore A. (2003), Analysis of glutathione: implication in redox and detoxification, Clin Chim Acta, 33, 19. ; Pourahmad J. (2000), A comparison of hepatocyte cytotoxic mechanisms for Cu<sup>+2</sup> and Cd<sup>+2</sup>, Toxicology, 143, 263. ; Pourahmad J. (2003), Carcinogenic metal induced reactive oxygen species formation in hepatocytes, Toxicol in Vitro, 17, 803. ; Robertson J. (2000), Molecular mechanisms of apoptosis induced by cytotoxic chemicals, Crit Rev Toxicol, 30, 609. ; Sedlak J. (1968), Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent, Anal Biochem, 25, 192. ; Sen C. (2000), Cellular thiols and redox-regulated signal transduction, Curr Top Cell Regul, 36, 1. ; Shaikh Z. (1999), Oxidative stress as a mechanism of chronic cadmium-induced hepatotoxicity and renal toxicity and protection by antioxidants, Toxicol Appl Pharmacol, 154, 256. ; Sies H. (1999), Glutathione and its role cellular functions, Free Radic Biol Med, 27, 916. ; Stohs S. (2001), Oxidative mechanisms in the toxicity of chromium and cadmium ions. J Environ, Pathol Toxicol Oncol, 20, 77. ; Tennant R. (1964), Evaluation of trypan blue technique for determination of cell viability, Transplantation, 2, 685. ; Ungar K (<b>1991</b>) Invitox Protocol Number 20. Isolation of Rat Hepatocytes. Invitox, Protocol ERGATT/FRAME Data bank of In Vitro Techniques in Toxicology. ; Waalkes M. (2003), Cadmium carcinogenesis, Mutat Res, 533, 107, ; Waisberg M. (2003), Molecular and cellular mechanisms of cadmium carcinogenesis, Toxicology, 192, 95. ; Wang Y. (2004), Cadmium inhibits the electron transfer chain and induces reactive oxygen species, Free Radic Biol Med, 11, 1434. ; Wendel A. (1981), Glutathione peroxidase, Methods Enzymol, 77, 325. ; Williams G. (2000), Oxidative DNA damage: endogenous and chemically induced, Regul Toxicol Pharmacol, 32, 283.