Details

Title

Evaluation of effectiveness of oleanolic acid in rat testicular ischemia-reperfusion injury model

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2026

Volume

Vol. 29

Issue

No. 1

Authors

Affiliation

Wei, S.-M. : Shulan International Medical College, Zhejiang Shuren University, 310015 Hangzhou City, China; School of Nursing, Zhejiang Chinese Medical University, 310053 Hangzhou City, China ; Huang, Y.-M. : Department of Sports Science, College of Education, Zhejiang University, 310058 Hangzhou City, China

Keywords

antioxidant ; ischemia-reperfusion ; oleanolic acid ; oxidative stress ; testicular torsion

Divisions of PAS

Nauki Biologiczne i Rolnicze

Coverage

55–62

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliography

  • Akhigbe RE, Odetayo AF, Akhigbe TM, Hamed MA, Ashonibare PJ (2024) Pathophysiology and management of testicular ischemia/reperfusion injury: Lessons from animal models. Heliyon 10: e27760.
  • Anderson E, Chaplin WJ, Turner C, Johnson GD, Blake H, Tabner A (2025) Experiences and perceptions of acute testicular pain, with a focus on reasons for delayed presentation to hospital: a qualitative evidence synthesis. Emerg Med J 42: 264-270.
  • Cai SC, Li XP, Li X, Tang GY, Yi LM, Hu XS (2021) Oleanolic acid inhibits neuronal pyroptosis in ischaemic stroke by inhibiting miR-186-5p expression. Exp Neurobiol 30: 401-414.
  • Chen Q, Xiao D, Wang Y, Zhang Z, Lin X, Ji Q, Han Y, Yu L, Xu J (2025) Neutrophil-mimetic oleanolic acid-loaded liposomes targeted to alleviate oxidative stress for renal ischemia-reperfusion injury treatment. Int J Pharm X 9: 100344.
  • Delgado-Miguel C, Arredondo-Montero J, Moreno-Alfonso JC, Garavis Montagut I, San Basilio M, Hernández I, Carrera N, Martínez L, Iraola E, Ruiz Jiménez I, Aguado Roncero P, Fuentes E, Díez R, Hernández-Oliveros F (2025) The role of neutrophil-to-lymphocyte ratio as a predictor of orchiectomy or testicular atrophy after torsion in children: a multicentric study. J Pers Med 15: 310.
  • Dhalla NS, Ostadal P, Tappia PS (2025) Involvement of oxidative stress and antioxidants in modification of cardiac dysfunction due to ischemia-reperfusion injury. Antioxidants (Basel) 14: 340.
  • Du Y, Ko KM (2006) Oleanolic acid protects against myocardial ischemia-reperfusion injury by enhancing mitochondrial antioxidant mechanism mediated by glutathione and alphatocopherol in rats. Planta Med 72: 222-227.
  • Gaber Ibrahim MF, Abdel-Hakeem EA, Shawky HA, Abdelkawy Abdelwahab S, Mokhemer SA (2026) Effect of local versus systemic exosomes administration on both testes in rat model of testicular ischemic reperfusion injury: shedding light on blood testis barrier. Tissue Barriers 14: 2532195.
  • Ghasemi M, Basiri A, Mohammad Jafari R, Shafaroodi H, Tavangar SM, Mohammadi Hamaneh A, Dehpour AR (2025) Protective effects of lithium against testicular ischemia-reperfusion injury: Involvement of the Akt/GSK-3β pathway. J Pediatr Urol 21: 558-566.
  • Hao BB, Pan XX, Fan Y, Lu L, Qian XF, Wang XH, Zhang F, Rao JH (2016) Oleanolic acid attenuates liver ischemia reperfusion injury by HO-1/Sesn2 signaling pathway. Hepatobiliary Pancreat Dis Int 15: 519-524.
  • Hu Z, Wang J, Ning J, Cheng F (2025) Curcumin mitigates testicular ischemia-reperfusion injury by suppressing NLRP3-mediated pyroptosis. Mol Immunol 185: 71-80.
  • Izanloo H, Soleimanzadeh A, Bucak MN, Imani M, Zhandi M (2021) The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5°C. Cryobiology 101: 12-19.
  • Johnsen SG (1970) Testicular biopsy score count – a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1: 2-25.
  • Karpinska B, Foyer CH (2024) Superoxide signalling and antioxidant processing in the plant nucleus. J Exp Bot 75: 4599-4610.
  • Kračun D, Lopes LR, Cifuentes-Pagano E, Pagano PJ (2025) NADPH oxidases: redox regulation of cell homeostasis and disease. Physiol Rev 105: 1291-1428.
  • Lukaczer D, Darland G, Tripp M, Liska D, Lerman RH, Schiltz B, Bland JS (2005) A pilot trial evaluating Meta050, a proprietary combination of reduced iso-alpha acids, rosemary extract and oleanolic acid in patients with arthritis and fibromyalgia. Phytother Res 19: 864-869.
  • Luo HQ, Shen J, Chen CP, Ma X, Lin C, Ouyang Q, Xuan CX, Liu J, Sun HB, Liu J (2018) Lipid-lowering effects of oleanolic acid in hyperlipidemic patients. Chin J Nat Med 16: 339-346.
  • Mao C, Yang C, Zhong H, Deng Q, Zhang T, Chu H, Shen J, Cao Y (2025) Development of a nomogram to predict risk factors for orchiectomy after testicular torsion in children. Sci Rep 15: 15154.
  • Matsushima S, Sadoshima J (2022) Yin and Yang of NADPH oxidases in myocardial ischemia-reperfusion. Antioxidants (Basel) 11: 1069.
  • Mdhluli MC, van der Horst G (2002) The effect of oleanolic acid on sperm motion characteristics and fertility of male Wistar rats. Lab Anim 36: 432-437.
  • Méndez-Valdés G, Pérez-Carreño V, Bragato MC, Hundahl M, Chichiarelli S, Saso L, Rodrigo R (2022) Cardioprotective mechanisms against reperfusion injury in acute myocardial infarction: targeting angiotensin II receptors. Biomedicines 11: 17.
  • Minich DM, Bland JS, Katke J, Darland G, Hall A, Lerman RH, Lamb J, Carroll B, Tripp M (2007) Clinical safety and efficacy of NG440: a novel combination of rho iso-alpha acids from hops, rosemary, and oleanolic acid for inflammatory conditions. Can J Physiol Pharmacol 85: 872-883.
  • Mohammadnehjad K, Mohammadi R, Soleimanzadeh A, Shalizar-Jalali A, Sarrafzadeh-Rezaei F (2025a) Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats. Vet Res Forum 16: 277-284.
  • Mohammadnejad K, Mohammadi R, Soleimanzadeh A, Shalizar-Jalali A, Sarrafzadeh-Rezaei F (2025b) The effect of β-cryptoxanthin on testicular ischemia-reperfusion injury in a rat model: evidence from testicular histology. Iran J Vet Surg 20: 110-115.
  • Mostahsan Z, Azizi S, Soleimanzadeh A, Shalizar-Jalali A (2024a) Protective effects of Mito-TEMPO on ischemiareperfusion injury in a mouse testicular torsion and detorsion model. Vet Res Forum 15: 665-672.
  • Mostahsan Z, Azizi S, Soleimanzadeh A, Shalizar-Jalali A (2024b) The protective roles of Mito-TEMPO on testicular ischemia-reperfusion injury based on biochemical and histopathological evidences in mice. Iran J Vet Surg 19: 97-105.
  • Pi Y, Zuo H, Wang Y, Zheng W, Zhou H, Deng L, Song H (2024) Oleanolic acid alleviating ischemia-reperfusion injury in rat severe steatotic liver via KEAP1/NRF2/ARE. Int Immunopharmacol 138: 112617.
  • Refaie MM, Abdel-Hakeem EA, Ahmed Ibrahim R, Hassan Mohamed Z, Shehata S, Soltan Shrekawy El Bakry H, Ali DM (2025) Role of sacubitril/valsartan in testicular ischaemia reperfusion injury via modulation of AngII/ASK/NF-κB and JAK/STAT pathways in juvenile rats. Br J Pharmacol 182: 4343-4362.
  • Santos-Lozano JM, Rada M, Lapetra J, Guinda Á, Jiménez-Rodríguez MC, Cayuela JA, Ángel-Lugo A, Vilches-Arenas Á, Gómez-Martín AM, Ortega-Calvo M, Castellano JM (2019) Prevention of type 2 diabetes in prediabetic patients by using functional olive oil enriched in oleanolic acid: The PREDIABOLE study, a randomized controlled trial. Diabetes Obes Metab 21: 2526-2534.
  • Sapkota A, Choi JW (2022) Oleanolic acid provides neuroprotection against ischemic stroke through the inhibition of microglial activation and NLRP3 inflammasome activation. Biomol Ther (Seoul) 30: 55-63.
  • Shakouri N, Soleimanzadeh A, Rakhshanpour A, Bucak MN (2021) Antioxidant effects of supplementation of 3,4-dihydroxyphenyl glycol on sperm parameters and oxidative markers following cryopreservation in canine semen. Reprod Domest Anim 56: 1004-1014.
  • Soleimanzadeh A, Karvani N, Davoodi F, Molaie R, Raisi A (2024) Efficacy of silver-doped carbon dots in chemical castration: a rat model study. Sci Rep 14:24132.
  • Tasci A, Uguralp S, Akatli AN, Arslan AK, Karabulut AB (2022) Long-term effects of orchiopexy and orchiectomy on the testes of rats with testicular torsion. J Pediatr Urol 18: 376.e1-376.e7.
  • Teschke R (2024) Hemochromatosis: Ferroptosis, ROS, gut microbiome, and clinical challenges with alcohol as confounding variable. Int J Mol Sci 25: 2668.
  • Wang W, Wu L, Li J, Ji J, Chen K, Yu Q, Li S, Feng J, Liu T, Zhang J, Chen J, Zhou Y, Mao Y, Wang F, Dai W, Fan X, Guo C, Wu J (2019) Alleviation of hepatic ischemia reperfusion injury by oleanolic acid pretreating via reducing HMGB1 release and inhibiting apoptosis and autophagy. Mediators Inflamm 2019: 3240713.
  • Wei J, Li Z, Wei Y, Tang D, Bai G, Men L, Wu S, Yan X (2025) Development and validation of a predictive model for testicular atrophy after orchiopexy in children with testicular torsion. World J Emerg Med 16: 387-391.
  • Wei SM, Huang YM (2024a) Effect of sulforaphane on testicular ischemia-reperfusion injury induced by testicular torsiondetorsion in rats. Sci Rep 14: 23420.
  • Wei SM, Huang YM (2024b) Safranal ameliorates testicular ischemia-reperfusion injury in testicular torsion-detorsion rat model. Rev Int Androl 22: 33-41.
  • Wei SM, Huang YM, Qin ZQ (2022) Salidroside exerts beneficial effect on testicular ischemia-reperfusion injury in rats. Oxid Med Cell Longev 2022: 8069152.
  • Yang J, Li X, Yang H, Long C (2021) Oleanolic acid improves the symptom of renal ischemia reperfusion injury via the PI3K/AKT pathway. Urol Int 105: 215-220.
  • Yildizhan E, Akkus M, Ulger BV, Asır F, Söker S, Gündüz E, Rençber M, Barçin M (2023) Protective effectiveness of anise against testicular ischemia and reperfusion injury: an experimental study in rats. Pol J Vet Sci 26: 249-255.
  • Zeynali E, Soleimanzadeh A, Azizi S (2025) Synergistic protection of alpha-glucosyl hesperidin and procyanidin against testicular ischemia-reperfusion injury. J Food Biochem 2025: 5634210.
  • Zolfaghari S, Soleimanzadeh A, Baqerkhani M (2025) The synergistic activity of fisetin on quercetin improves testicular recover in ischemia-reperfusion injury in rats. Sci Rep 15: 12053.

Date

17.03.2026

Type

Article

Identifier

DOI: 10.24425/pjvs.2026.158501
×