Details
Title
Optimized high-yield expression and antigenic characterization of a soluble pseudorabies virus gE protein in E. coliJournal title
Polish Journal of Veterinary SciencesYearbook
2026Volume
Vol. 29Issue
No. 1Authors
Affiliation
Wang, Y.B. : School of Public Health, Henan Medical University, Xinxiang, Henan, China ; Wang, S.H. : School of Public Health, Henan Medical University, Xinxiang, Henan, China ; Gao, Y.C. : School of Public Health, Henan Medical University, Xinxiang, Henan, China ; Feng, J.W. : School of Public Health, Henan Medical University, Xinxiang, Henan, China ; Zhang, F.Q. : School of Public Health, Henan Medical University, Xinxiang, Henan, ChinaKeywords
pseudorabies virus ; optimization of expression conditions ; water-soluble gE protein ; antigenicity characterizationDivisions of PAS
Nauki Biologiczne i RolniczeCoverage
91–100Publisher
Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in OlsztynBibliography
- Ai J, Weng S, Cheng Q, Cui P, Li Y, Wu H, Zhu Y, Xu B, Zhang W (2018) Human endophthalmitis caused by pseudorabies virus infection, China, 2017. Emerg Infect Dis 24: 1087-1090.
- Ao J, Wang J, Chen X, Wang X, Long Q (2003) Expression of pseudorabies virus gE epitopes in Pichia pastoris and its utilization in an indirect PRV gE-ELISA. J Virol Methods 114: 145-150.
- Bjerga GE, Lale R, Williamson AK (2016) Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes. Bioengineered 7: 33-38.
- Bo Z, Li X (2022) A review of pseudorabies virus variants: genomics, vaccination, transmission, and zoonotic potential. Viruses 14: 1003.
- Bude SA, Lu Z, Zhao Z, Zhang Q (2024) Pseudorabies virus glycoproteins E and B application in vaccine and diagnosis kit development. Vaccines (Basel) 12: 1078.
- Costa SJ, Almeida A, Castro A, Domingues L, Besir H (2013) The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology. Appl Microbiol Biotechnol 97: 6779-6791.
- Crowther JR (2000) The ELISA guidebook. In: Methods Mol Biol. The Humana Press, USA, pp 1-448.
- Eskandari A, Nezhad NG, Leow TC, Rahman MB, Oslan SN (2024) Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli. Arch Microbiol 206: 152.
- Gómez-Sebastián S, Pérez-Filgueira DM, Gómez-Casado E, Nuñez MC, Sánchez-Ramos I, Tabarés E, Escribano JM (2008) DIVA diagnostic of Aujeszky’s disease using an insect-derived virus glycoprotein E. J Virol Methods 153: 29-35.
- Gopal GJ, Kumar A (2013) Strategies for the production of recombinant protein in Escherichia coli. Protein J 32: 419-425.
- Gupta SK, Shukla P (2015) Advanced technologies for improved expression of recombinant proteins in bacteria: perspectives and applications. Crit Rev Biotechnol 36: 1089-1098.
- Hayat SM, Farahani N, Golichenari B, Sahebkar A (2018) Recombinant protein expression in Escherichia coli (E.coli): what we need to know. Curr Pharm Des 24: 718-725.
- Hou Y, Wang Y, Zhang Y, Yu H, Zhao Y, Yi A (2022) Human encephalitis caused by pseudorabies virus in China: a case report and systematic review. Vector Borne Zoonotic Dis 22: 391-396.
- Huleani S, Roberts MR, Beales L, Papaioannou EH (2021) Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression. Crit Rev Biotechnol 42: 756-773.
- Kaur J, Kumar A, Kaur J (2018) Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements. Int J Biol Macromol 106: 803-822.
- Liu Q, Wang X, Xie C, Ding S, Yang H, Guo S, Li J, Qin L, Ban F, Wang D, Wang C, Feng L, Ma H, Wu B, Zhang L, Dong C, Xing L, Zhang J, Chen H, Yan R, Wang X, Li W (2022) A novel human acute encephalitis caused by pseudorabies virus variant strain. Clin Infect Dis 74: 756.
- Lozano Terol G, Gallego-Jara J, Sola Martínez RA, Martínez Vivancos A, Cánovas Díaz M, de Diego Puente T (2021) Impact of the expression system on recombinant protein production in Escherichia coli BL21. Front Microbiol 12: 682001.
- Ma L, Wang Y, Wang X, Zhang M, Zhu M (2024) An isothermal recombinase polymerase assay coupled with lateral flow dipstick for differentiation of pseudorabies virus wild isolates and gE-deleted vaccine strains. Pol J Vet Sci 27: 621-630.
- Martínez-Alonso M, Vera A, Villaverde A (2007) Role of the chaperone DnaK in protein solubility and conformational quality in inclusion body-forming Escherichia coli cells. FEMS Microbiol Lett 273: 187-195.
- Mettenleiter TC (2000a) Aujeszky’s disease (pseudorabies) virus: the virus and molecular pathogenesis – state of the art, June 1999. Vet Res 31: 99-115.
- Mettenleiter TC (2020b) Aujeszky’s disease and the development of the marker/DIVA vaccination concept. Pathogens 9: 563.
- Morenkov O, Fodor N, Fodor I (1999) Indirect ELISAs based on recombinant and affinity-purified glycoprotein E of Aujeszky’s disease virus to differentiate between vaccinated and infected animals. Acta Vet Hung 47: 137-150.
- Ou J, Cai S, Zheng F, Lu G, Zhang G (2020) Human pseudorabies virus infection: a new threat in China. J Infect 80: 578-606.
- Polcicova K, Goldsmith K, Rainish BL, Wisner TW, Johnson DC (2005) The extracellular domain of herpes simplex virus gE is indispensable for efficient cell-to-cell spread: evidence for gE/gI receptors. J Virol 79: 11990-12001.
- Pomeranz LE, Reynolds AE, Hengartner CJ (2005) Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev 69: 462-500.
- Rosano GL, Ceccarelli EA (2014) Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol 5: 172.
- Serena MS, Geisler C, Metz GE, Corva SG, Mórtola EC, Larsen A, Jarvis DL, Echeverría MG (2013) Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs. Protein Expr Purif 90: 1-8.
- Terpe K (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 72: 211-222.
- Tischer BK, Osterrieder N (2010) Herpesviruses – a zoonotic threat? Vet Microbiol 140: 266-270.
- Upadhyay AK, Murmu A, Singh A, Panda AK (2012) Kinetics of inclusion body formation and its correlation with the characteristics of protein aggregates in Escherichia coli. PLoS One 7: e33951.
- Wang M, Wang L, Zhao Q (2019) Efficacy evaluation of two live virus vaccines against an emerging pseudorabies virus variant. Pol J Vet Sci 22: 639-645.
- Wang Y, Guo J, Qiao S, Li Q, Yang J, Jin Q, Zhang G (2016) GP5 protein-based ELISA for the Detection of PRRSV Antibodies. Pol J Vet Sci 19: 495-501.
- Wang Y, Qiao S, Li X, Xie W, Guo J, Li Q, Liu X, Hou J, Xu Y, Wang L, Guo C, Zhang G (2015) Molecular epidemiology of outbreak-associated pseudorabies virus (PRV) strains in central China. Virus Genes 50: 401-409.
- Wang YB, Li P, Gao YC, Hao PF, Feng JW, Hu NY, Cao J, Hu JH, Ding K, Wang L (2024) Development of a capsid protein-based ELISA for the detection of PCV2 antibodies in swine serum. Pol J Vet Sci 27: 529-529.
- Wang YB, Wang SW, Jin QY, Chen LP, Zhang FQ, Shi JJ, Yin Y, Fan ZX, Liu XY, Wang LP, Li P (2023) Expression of water-soluble nucleocapsid protein of SARS-CoV-2 and analysis of its immunogenicity. Pol J Vet Sci 26: 571-579.
- Wingfield PT, Palmer I, Liang SM (2014) Folding and purification of insoluble (inclusion body) proteins from Escherichia coli. Curr Protoc Protein Sci 78: 6.5.1-6.5.30.
- Wu CY, Wu CW, Liao CM, Chien MS, Huang C (2017) Enhancing expression of the pseudorabies virus glycoprotein E in yeast and its application in an indirect sandwich ELISA. J Appl Microbiol 123: 594-601.
- Yong T, Huan-chun C, Shao-bo X, Ya-li Q, Qi-gai H, Yu-qi R (2005) Development of a latex agglutination test using the major epitope domain of glycoprotein E of pseudorabies virus expressed in E. coli to differentiate between immune responses in pigs naturally infected or vaccinated with pseudorabies virus. Vet Res Commun 29: 487-497.