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Polish Journal of Veterinary Sciences

Polish Journal of Veterinary Sciences

Opis

The Polish Journal of Veterinary Sciences is an open-access peer-reviewed journal (http://pjvsci.uwm.edu.pl/). It was established in 1998 and has been published continuously as a quarterly since then.

The Journal is aimed at publishing high-quality research (original and short communication) and review articles from the wide field of veterinary medicine. English-language articles from a variety of areas are welcome.

The following topics are particularly encouraged: new knowledge on the pathophysiology of animal diseases; new knowledge on animal disease therapy, therapeutics and prevention; new knowledge on animal-associated public health issues.

The Journal is indexed by Thomson Scientific (BIOSIS/Biological Records, Biotechnology Citation Index®, Current Contents®/Biology, Agriculture and Environmental Sciences, Science Citation Index® Expanded), Index Copernicus, the National Library of Medicine (PubMed/ MEDLINE®) and the Polish Scientific Journal Contents.

The Journal is owned in equal parts by the Polish Academy of Sciences Committee of Veterinary Sciences and by the University of Warmia and Mazury in Olsztyn.

Submissions are evaluated by reviewers using the single-blind peer review process. The policy of the journal is to accept articles only after a positive peer review.


Journal Metrics:

Impact Factor 2022: 0.8

CiteScore 2022: 1.5

SCImago Journal Rank (SJR) 2022: 0.242

Source Normalized Impact per Paper (SNIP) 2022: 0.346

ISSN
ISSN 1505-1773
Wydawcy
Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn

Editor-in-Chief:

Michał Załęcki, University of Warmia and Mazury, Faculty of Veterinary Medicine, Department of Animal Anatomy, Olsztyn, Poland

Deputy/ Managing Editors:

Kaleczyc Jerzy, University of Warmia and Mazury, Faculty of Veterinary Medicine, Department of Animal Anatomy, Olsztyn, Poland

Krzysztof Wąsowicz, University of Warmia and Mazury, Faculty of Veterinary Medicine, Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Olsztyn, Poland

Polish Journal of Veterinary Sciences

University of Warmia and Mazury

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Articles are published in open access under CC BY-NC-ND 4.0 license ( https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en), which allows re-users to copy and distribute the material in any medium or format in unadapted form and for noncommercial purposes, and only so long as attribution is given to the creator. In simple words: others can download published works and share them with others as long as they credit Authors, but they can’t change the articles in any way or use them commercially. Pease note: Under the CC BY-NC-ND license reuse is only applicable for non-commercial purposes. To obtain permission for commercial use please contact Editorial Office.




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Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4

1 Comparison of the results of sleeve gastrectomy, gastric pilication and liragulitide in obese rats
H. Bilge , O. Basol , E. Yıldızhan , B.V. Ulger , H. Temiz , M. Akkus , I. Yıldızhan
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 535-540 | DOI: 10.24425/pjvs.2023.145059
Słowa kluczowe: obesity liraglutide sleeve gastrectomy gastric plication
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Abstrakt

Obesity, which is generally seen in adults, is a serious health problem. Diseases caused by obesity are among the leading causes of death worldwide. Liraglutide (LG) is an analogue of glucagon-like peptide-1, which slows gastrointestinal motility, resulting in decreased food consumption. Gastric plication (GP) and sleeve gastrectomy (SG) is the reduction of stomach volume by surgical means. We examined and compared the body mass index (BMI) changes, metabolic changes and changes in gastric histology in obese rats after LG injection with surgical methods such as SG and GP.
In this research, 35 Wistar Albino female rats were used. Rats were divided into 5 groups with 7 rats in each group. Group (G) 1: The control group, fed with a normal calorie diet for 8 weeks. G 2: Sham group, G 3: SG group, G 4: GP group and G 5: LG group, fed with high-calorie feed for 4 weeks. At the end of the 4th week, the study was terminated by making appropriate interventions for the groups.
When the blood glucose (BG) levels measured at the beginning, 4 th week and 8 th week of the experiment were evaluated, it was monitored that the BG level at the 8 th week was the lowest in the LG group (p<0.05). It was observed that the preop Ghrelin and Leptin levels of the LG group were lower than those of the SG and GP groups (p<0.05). As a consequenc
As a consequence of our metabolic investigations, we observed that the use of LG is at least as effective as SG.

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Bibliografia

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Autorzy i Afiliacje

H. Bilge
1
O. Basol
1
E. Yıldızhan
2
B.V. Ulger
3
H. Temiz
4
M. Akkus
2
I. Yıldızhan
5
  1. Department of General Surgery, SBU Gazi Yaşargil Training and research Hospital, Elazığ Road 10.Km Üçkuyular location 21070, Diyarbakır, Turkey
  2. Dicle University, Faculty of Medicine, Department of Histology and Embryology, Kıtılbıl Mah. 21280, Diyarbakır, Turkey
  3. Dicle University, Faculty of Medicine, Department of General Surgery, Kıtılbıl Mah. 21280, Diyarbakır, Turkey
  4. Dicle University, Faculty of Medicine, Department of Microbiology, Kıtılbıl Mah. 21280, Diyarbakır, Turkey
  5. Iğdır University, Faculty of Agricultural Technology, Department of Agricultural Technology, Şehit Bülent Yurtseven Kampüsü, & Karaağaç Kampüsü, 76000 Iğdır, Turkey
2 The occurrence and seasonal variation of aflatoxin B1 and zearalenone concentrations in poultry feeds
A. Sohrabi , M.H. Movassaghghazani , J. Shayegh , A.R. Karamibonari , F. Tajedini
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 541-548 | DOI: 10.24425/pjvs.2023.145060
Słowa kluczowe: aflatoxin B1 high-performance liquid chromatography poultry diet zearalenone
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Abstrakt

The present study aimed to investigate the contamination of poultry feed with aflatoxin B1 and zearalenone at laying hen farms in Tehran suburbs. The poultry feed was selected from five laying hen farms. A total of 60 poultry feed samples were collected from each farm during four consecutive seasons, from spring to winter of 2021. High-performance liquid chromatography was used to determine the amount of aflatoxin B1 and zearalenone. The mean aflatoxin B1 and zearalenone concentrations in various seasons showed significant differences (p<0.01). The highest reported aflatoxin concentration was in winter, with a mean concentration of 1366.53±77.85 ng/kg. The lowest concentrations were reported in autumn and summer, indicating a significant difference (p<0.01). The highest concentration of zearalenone was reported in summer, with a mean concentration of 150.72±10.35 μg/kg. The lowest concentration was reported in winter, with a mean concentration of 22.87±10.35 μg/kg, indicating a statistically significant difference (p<0.01). Overall, the concentrations of aflatoxin B1 and zearalenone toxins significantly differed in various poultry farms. The poultry farm D had the highest aflatoxin contamination with a mean concentration of 648.08±59.89 ng/kg. Poultry farms A, B, and C had the highest zearalenone concentrations with mean concentrations of 125.17±20.61, 96.04±20.61, and 99.49±20.61 μg/kg, respectively. Autumn was the only season showing significant differences regarding zearalenone toxin concentration in poultry farms.
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Autorzy i Afiliacje

A. Sohrabi
1
M.H. Movassaghghazani
2
J. Shayegh
3
A.R. Karamibonari
3
F. Tajedini
4
  1. Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  2. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  3. Department of Pathobiology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  4. Department of Basic Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
3 Comparative evaluation of single and combined efficacy of dipyridamole, ketotifen and quercetin on cyclosporine induced hepatorenal toxicity
B. Dik , T.M. Parlak , O. Tufan , F.B. Ozgur , A. Er
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 549-557 | DOI: 10.24425/pjvs.2023.148275
Słowa kluczowe: hepatorenal toxicity cyclosporine ketotifen dipyridamole quercetin
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Abstrakt

Cyclosporine is an immunosuppressive drug that is used to prevent tissue rejection in organ transplants and to treat autoimmune diseases such as psoriasis and rheumatoid arthritis. It has important toxic effects in many organs such as the liver and kidney. The aim of this study was to determine and compare the effectiveness of the single and combined treatment of dipyridamole, which is a vasodilator and has an antioxidant effect, ketotifen which is toll-like receptor-4 inhibitory and has an antioxidant effect, quercetin which is an antioxidant and has an anti-inflammatory effect in cyclosporine-induced hepatorenal toxicity. Forty-eight Wistar Albino rats were divided into 7 groups. The research period was 21 days. The cyclosporine increased serum ALT and AST levels, in contrast to their increased levels prevented by all the treatments. The serum creatinine level decreased significantly with ketotifen and combined treatment, while cyclosporine partially increased serum creatinine and urea levels. The urine microalbumin and protein levels were increased significantly by cyclosporine, whereas they decreased with dipyridamole treatment. The protein levels decreased by quercetin and combined treatments. The kidney injury molecule- 1 and retinol-binding protein levels were increased by the cyclosporine, while ketotifen treatment partially decreased them. In conclusion, ketotifen and dipyridamole can prevent cyclosporine- induced hepatorenal toxicity and quercetin can increase the effectiveness of this treatment.
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Autorzy i Afiliacje

B. Dik
1
T.M. Parlak
1
O. Tufan
1
F.B. Ozgur
2
A. Er
1
  1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Selcuk University, Ardicli Neighborhood, 42130, Konya, Turkey
  2. Harran University, Birecik Vocational School, Laboratory and Veterinary Health Program, Karsiyaka Neighborhood, Prof. Dr. İbrahim Halil MUTLU Avenue, 63400, Sanliurfa, Turkey
4 Evaluation of the clinical efficacy of racecadotril in the treatment of neonatal calves with infectious diarrhea
B. Tras , M. Ok , M. Ider , T.M. Parlak , R. Yildiz , H. Eser Faki , Z. Ozdemir Kutahya , K. Uney
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 559-569 | DOI: 10.24425/pjvs.2023.148276
Słowa kluczowe: neonatal calf infectious diarrhea racecadotril treatment
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Abstrakt

Racecadotril, used as an antidiarrheal drug in humans and some animals such as the dog, inhibits peripheral enkephalinase, which degrades enkephalins and enkephalinase inhibition induces a selective increase in chloride absorption from the intestines. The study material consisted of 46 calves with infectious diarrhea and 14 healthy calves in the age 2-20 days. The calves were divided into eight groups; healthy calves (HG), healthy calves administered racecadotril (HRG), calves with E.coli-associated diarrhea (ECG), calves with E.coli-associated diarrhea administered racecadotril (ECRG), calves with bovine Rotavirus/Coronavirus-associated diarrhea (VG), calves with bovine Rotavirus/Coronavirus-associated diarrhea administered racecadotril (VRG), calves with C. parvum-associated diarrhea (CG) and calves with C. parvum-associated diarrhea administered racecadotril (CRG). Calves in the racecadotril groups received oral racecadotril at a dose of 2.5 mg/kg twice a day for 3 days. A routine clinical examination of all calves was performed. Hemogram and blood gas measurements were made from the blood samples. Standard diarrhea treatment was applied to the HG, ECG, CG, and VG groups. Clinical score parameters such as appetite, feces quality, dehydration, standing and death and some blood gas and hemogram parameters were evaluated to determine the clinical efficacy of racecadotril. Clinical score parameters were determined observationally. Blood gas measurements were performed using a blood gas analyzer. The hemogram was performed using an automated hematologic analyzer. Statistically significant differences were determined in the blood pH, bicarbonate, base deficit, lactate, and total leukocyte count in calves with diarrhea compared to healthy calves. After the treatments, these parameters were found to be within normal limits. At the end of treatment, 42 of the 46 diarrheal calves recovered, while 4 died. We found that racecadotril was effective in improving both clinical recovery and feces consistency in neonatal calves with diarrhea caused by E. coli. As a result, it can be stated that racecadotril, which has an antisecretory effect, is beneficial in the treatment of bacterial diarrhea caused by such as E. coli.
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Bibliografia

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Autorzy i Afiliacje

B. Tras
1
M. Ok
2
M. Ider
2
T.M. Parlak
1
R. Yildiz
3
H. Eser Faki
1
Z. Ozdemir Kutahya
4
K. Uney
1
  1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Ardicli Neighborhood, 42100, Konya, Turkey
  2. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Selcuk, Ardicli Neighborhood, 42100, Konya, Turkey
  3. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Yakakoy, 15030, Burdur, Turkey
  4. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Cukurova, Fatih Sultan Mehmet Avenue, 01930, Adana, Turkey
5 Expression of water-soluble nucleocapsid protein of SARS-CoV-2 and analysis of its immunogenicity
Y.B. Wang , S.W. Wang , Q.Y. Jin , L.P. Chen , F.Q. Zhang , J.J. Shi , Y. Yin , Z.X. Fan , X.Y. Liu , L.P. Wang , P. Li
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 571-579 | DOI: 10.24425/pjvs.2023.148277
Słowa kluczowe: SARS-CoV-2 optimization of expression conditions water-soluble nucleocapsid protein immunogenicity analysis
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Abstrakt

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be a major public health concern. Nucleocapsid (N) protein is the most abundant structural protein on SARS-CoV-2 virions and induces the production of antibodies at the early stage of infection. Large-scale preparation of N protein is essential for the development of immunoassays to detect antibodies to SARS-CoV-2 and the control of virus transmission. In this study, expression of water-soluble N protein was achieved through inducing protein expression at 25°C with 0.5 mM IPTG for 12 h. Western blot and ELISA showed that recombinant N protein could be recognized by sera collected from subjects immunized with Sinovac inactivated SARS-CoV-2 vaccine. Four monoclonal antibodies namely 2B1B1, 4D3A3, 5G1F8, and 7C6F5 were produced using hybridoma technology. Titers of all four monoclonal antibodies in ELISA reached more than 1.28×10 6.0. Moreover, all monoclonal antibodies could react specifically with N protein expressed by transfection of pcDNA3.1-N into BHK-21 cells in IPMA and IFA. These results indicated that water-soluble N protein retained high immunogenicity and possessed the same epitopes as that of native N protein on virions. In addition, the preparation of water-soluble N protein and its monoclonal antibodies laid the basis for the development of immunoassays for COVID-19 detection.
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Bibliografia

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Autorzy i Afiliacje

Y.B. Wang
1
S.W. Wang
2
Q.Y. Jin
3
L.P. Chen
4
F.Q. Zhang
1
J.J. Shi
1
Y. Yin
5
Z.X. Fan
1
X.Y. Liu
6
L.P. Wang
6
P. Li
6
  1. School of Public Health, Xinxiang Medical University, Xinxiang 453003, P.R. China
  2. School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, P.R. China
  3. Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China
  4. Gushi County Center for Animal Disease Control and Prevention, Xinyang 465200, P.R. China
  5. Mingde College of Xinxiang Medical University, Xinxiang 453003, P.R. China
  6. School of Biological Engineering, Xinxiang University, Xinxiang 453003, P.R. China
6 Study of autonomic modulation by non-linear analysis of heart rate variability in different age groups and analysis of health status, disease and risk of death in dogs
L. Martinello , F.G. Romão , M.F. Godoy , L.H.A. Machado , M.H. Tsunemi , M.L.G. Lourenço
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 581-590 | DOI: 10.24425/pjvs.2023.148278
Słowa kluczowe: dogs sympathetic system parasympathetic system and heart rate variability
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Abstrakt

The symbolic analysis of heart rate variability (biomarker of cardiac autonomic homeostasis) is a nonlinear and effective tool for pattern extraction and classification in a series analysis, which implies the transformation of an original time series into symbols, represented by numbers. Autonomic heart rate control is influenced by different factors, and better indicators of heart rate variability are found in healthy young individuals than in older and sicker individuals. The aim of this study was to compare the indicators of heart rate variability among healthy dogs in different age groups and in health status using the nonlinear method of symbolic analysis to evaluate the diagnostic accuracy of this method for the risk of death in dogs. An increase in cardiac sympathetic modulation was observed in puppies and dogs at risk of death, which was evidenced by a marked increase of 0 V% (without variation – associated with sympathetic modulation) and a decrease in patterns of 2 V% (two variations – associated with parasympathetic modulation), while the opposite was observed in young adult dogs with increased parasympathetic modulation. Elderly dogs showed a gradual decrease in parasympathetic activity, which tended to worsen with loss of health. It is concluded that the variables of symbolic analysis may be useful to evaluate autonomic modulation in dogs and assist in the differentiation between health states, advanced disease and death throughout the life cycle and have been shown to be indices with high specificity, sensitivity and diagnostic accuracy to help identify dogs at risk of death.
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Bibliografia

1. Baisan RA, Vulpe V, Ohad DG (2021) Short-term heart rate variability in healthy dogs and dogs in various stages of degenerative mitral valve disease evaluated before pharmacotherapy. J Vet Cardiol 274: 1-6.
2. Barbosa MPCR, Silva NT, Azevedo FM, Pastre CM, Vanderlei CM (2016) Comparison of Polar RS800G3TM heart rate monitor with Polar S810iTM and electrocardiogram to obtain the series of RR intervals and analysis of heart rate variability at rest. Clin Physiol Funct Imaging 36: 112-117.
3. Bogucki S, Noszczyk-Nowak A (2017) Short-term heart rate variability in dogs with sick sinus syndrome or chronic mitral valve disease as compared to healthy Controls. Pol J Vet Sci 20: 167-172.
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Autorzy i Afiliacje

L. Martinello
1
F.G. Romão
1
M.F. Godoy
2
L.H.A. Machado
1
M.H. Tsunemi
3
M.L.G. Lourenço
1
  1. São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science
  2. Department of Cardiology and Cardiovascular Surgery – São José do Rio Preto Medical School (FAMERP)
  3. São Paulo State University (Unesp), Institute of Biosciences, Botucatu, São Paulo, Brazil
7 Teriflunomide induces Foxp3 expression in murine CD8 + T cells while IL-27 and retinoic acid exert a synergistic effect on the induction of CD39 expression on these cells
T. Maślanka , A. Jasiecka-Mikołajczyk
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 591-609 | DOI: 10.24425/pjvs.2023.148279
Słowa kluczowe: CD8+ T cells Treg cells Foxp3 IL-27 ATRA teriflunomide
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Abstrakt

The purpose of this study was to verify the possibility of pharmacological induction of Foxp3 +CD25 +CD8 + and Foxp3 -CD103 +CD8 + T regulatory cells ‘armed’ with immunosuppressive molecules, i.e. CD39 and IL-10. To achieve this purpose, stimulated and unstimulated murine lymphocytes were exposed to IL-27, teriflunomide (TER) and all trans retinoic acid (ATRA). The study found that: (a) IL-27 induced CD39 expression on Foxp3 +CD25 +CD8 + T cells and the ability of CD103+Foxp3-CD8+ T cells to produce IL-10 as well as increasing the absolute number of IL-10 +CD103 +Foxp3 -CD8 + T cells; (b) TER induced Foxp3 expression in CD25+CD8+ T cells and CD103 expression on Foxp3 -CD8 + T cells as well as increasing the absolute number of Foxp3 +CD25 +CD8 + T cells; (c) ATRA induced the capacity of Foxp3 +CD25 +CD8 + T cells to produce IL-10. The following desired interactions were demonstrated between IL-27 and ATRA: (a) a strong synergistic effect with respect to increasing CD39 expression and the ability to produce IL-10 by Foxp3 +CD25 +CD8 + T cells; (b) a synergistic effect with respect to increasing the absolute count of CD39 +Foxp3 +CD25 +CD8 + T cells. The study revealed that TER abolished all these effects. Therefore, a combination of the tested agents did not induce the generation of Foxp3 +CD25 +CD8 + and Foxp3 -CD103+CD8+ T cells characterized by extensive CD39 expression and IL-10 production. Thus, in the context of the pharmacological induction of IL-10 +CD39 +Foxp3 +CD25 +CD8 + and IL-10 +CD103 +Foxp3 -CD8 + T cells, these findings strongly suggest that a combination of TER with IL-27 and/or ATRA does not provide any benefits over TER alone; moreover, such a combination may result in abolishing the desired effects exerted by IL-27 and/or ATRA.
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Bibliografia

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Autorzy i Afiliacje

T. Maślanka
1
A. Jasiecka-Mikołajczyk
1
  1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
8 Expression and self-assembly of virus-like particles from porcine parvovirus and its application in antibody detection
Y. Li , Q. Wang , W. Yue , X. Li , Y. Chen , Y. Gao
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 611-613 | DOI: 10.24425/pjvs.2023.148280
Słowa kluczowe: porcine parvovirus virus-like particles indirect ELISA
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Abstrakt

Porcine parvovirus (PPV) is a major causative agent in reproductive pig disease. The swine industry faces a significant economic and epizootic threat; thus, finding a reliable, quick, and practical way to detect it is essential. In this investigation, recombinant PPV VP2 protein was expressed in the Escherichia coli ( E. coli) expression systems. As shown by electron microscopy (TEM), Western blot, and hemagglutination (HA) assays, the recombinant VP2 protein was successfully assembled into virus-like particles (VLPs) after being expressed and purified. These VLPs had a structure that was similar to that of real PPV viruses and also exhibited HA activity. These VLPs induced high levels of PPV-specific antibody titers in mice after immunization, indicating that the VLPs may be beneficial as potential candidate antigens. VLPs were used as the coating antigens for the VLP ELISA, and the PPV VLPs-based ELISA displayed a high sensitivity (99%), specificity (93.0%) and agreement rate (98.3%) compared to HI assay, and the agreement rate of this ELISA was 97.5% compared to a commercial ELISA kit. Within a plate, the coefficient of variation (CV) was 10%, and between ELISA plates, the CV was 15%. According to a cross-reactivity assay, the technique was PPV-specific in contrast to other viral illness sera. The PPV VLP indirect-ELISA test for PPV detection in pigs with an inactivated vaccine showed that the PPV-positive rate varied among different sample sources from 88.2 to 89.6%. Our results indicate that this ELISA technique was quick, accurate, and repeatable and may be used for extensive serological research on PPV antibodies in pigs.
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Bibliografia

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Autorzy i Afiliacje

Y. Li
1
Q. Wang
2
W. Yue
1
X. Li
1
Y. Chen
1
Y. Gao
1
  1. Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China
  2. State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
9 Nitric oxide production by spermatozoa and sperm characteristics in dogs with benign prostatic hyperplasia
A. Domoslawska-Wyderska , A. Orzołek , S. Zduńczyk , A. Rafalska
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 621-628 | DOI: 10.24425/pjvs.2023.148281
Słowa kluczowe: dog benign prostatic hyperplasia nitric oxide semen
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Abstrakt

The aim of this study was to evaluate the effect of benign prostatic hyperplasia (BPH) on nitric oxide (NO) production by spermatozoa and sperm parameters in dogs. The study was conducted on 40 intact dogs of various breeds. The dogs were assigned to two groups: BPH group (n=20) and non-affected group (n=20). The sperm concentration and motility parameters of spermatozoa were assessed using computer-assisted sperm analysis. For the assessment of sperm morphology monochromatic Diff-Quick stain was used. Plasma membrane integrity, mitochondrial membrane potential and the spermatozoa producing nitric oxide and with apoptotic-like changes were determined using fluorescent stain methods. The percentages of motile sperm, sperm with progressive motility and normal sperm were statistically significantly (p<0.05) lower in dogs with BPH than in non-affected dogs. The proportion of sperm in motility subcategory RAPID was statistically significantly (p<0.05) lower in dogs with BPH than in control dogs, whereas in the STATIC motility subcategory the proportion was significantly (p<0.05) higher in dogs with BPH. The percentage of spermatozoa producing NO was significantly (p<0.05) higher in dogs with BPH than in control dogs. In conclusion, the results of this study showed that BPH adversely affects semen quality, especially motility, in dogs. The decreased semen quality was associated with an increased proportion of spermatozoa generating NO. Further research is needed to clarify the mechanisms by which BPH affects semen quality.
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Autorzy i Afiliacje

A. Domoslawska-Wyderska
1
A. Orzołek
2
S. Zduńczyk
1
A. Rafalska
1
  1. Department of Animal Reproduction with Clinic, University of Warmia and Mazury, Oczapowskiego 14, 10-719 Olsztyn, Poland
  2. Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland
10 PCR-based methods in detection and identification of dermatophytes in dogs and cats with suspected dermatophytosis in 2021 in Poland
Dawid Jańczak , Piotr Górecki , Aleksandra Kornelia Maj
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 629-634 | DOI: 10.24425/pjvs.2023.148282
Słowa kluczowe: dermatophytes dogs cats Poland nested PCR multiplex PCR
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Abstrakt

Dermatophytes from Microsporum, Trichophyton and Epidermophyton genera are divided into geophilic, zoophilic and anthropophilic species which cause skin infection in humans and wide group of animals, mainly mammals. Main species causing dermatophytosis in dogs and cats are Microsporum and Trichophyton. Conventional mycological diagnostic technique includes Saburaud Dextrose Agar (SAD) and others medium cultures, 10% KOH mount and direct microscopy of hairs and scraping. Molecular diagnostic become more frequent in veterinary practice due to shortening of waiting time. In this study we based on two PCR methods. The nested PCR amplified CHS1 gene for dermatophytes detection, and multiplex PCR coding ITS1 and ITS2 fragments for species identification of detected derpatophytes. Most frequently detected species was Microsporum canis, mainly in young cats. Geophilic Microsporum gypseum and anthropophilic Trichophyton rubrum was found primarily in dogs. Molecular methods in dermatophytosis identification are rapid in contrast to routinely, long lasting culture.
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Bibliografia

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Autorzy i Afiliacje

Dawid Jańczak
1
Piotr Górecki
1
Aleksandra Kornelia Maj
1
  1. Animallab Veterinary Laboratory, Środkowa 2/4, 03-430 Warsaw, Poland
11 ABHD2 deficiency aggravates ovalbumin-induced airway remodeling through the PI3K/Akt pathway in an animal model of chronic asthma
L. Qiang , X. Li , Q. Li , H. Bo , Y. Liu , M. Lv , X. Chen , H. Ju , X. Sang , Z. Li , S. Jin
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 635-646 | DOI: 10.24425/pjvs.2023.148283
Słowa kluczowe: Abhd2 airway remodeling, inflammation PI3K/Akt signalling pathway
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Abstrakt

Airway remodeling is a major pathological characteristic of chronic obstructive pulmonary disease (COPD). This study aimed to investigate the effect of Abhd2 deficiency on ovalbumin (OVA)-induced airway remodeling and inflammation in vivo. Abhd2-deficient mice were used to establish an OVA-induced asthma model. Lung tissues were analyzed using hematoxylin and eosin (HE) staining, Masson staining, immunohistochemistry, quantitative reverse transcription- polymerase chain reaction (qRT-PCR), and western blotting were used to determine the role of Abhd2 in the regulation of OVA-induced airway remodeling and inflammation. Our findings revealed that the RNA expression of inflammatory factors, including IL-1β, IL-6, IL-4, and IL-13, was significantly increased in OVA-induced Abhd2 Gt/Gt asthmatic mice. The expression of IFN-γ was decreased significantly in OVA-induced Abhd2 Gt/Gt asthmatic mice. The protein expression of airway remodeling factors, including α-SMA, type I collagen, and Ki67, was also increased in OVA-induced Abhd2 Gt/Gt asthmatic mice compared to that in OVA-induced wild-type (WT) mice. Additionally, Abhd2 deficiency promoted the expression of p-Akt in tissues of the asthma model. These results suggest that Abhd2 deficiency exacerbates airway remodeling and inflammation through the PI3K/Akt pathway in chronic asthma.
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Bibliografia

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Autorzy i Afiliacje

L. Qiang
1
X. Li
1
Q. Li
2
H. Bo
3
Y. Liu
1
M. Lv
1
X. Chen
1
H. Ju
1
X. Sang
1
Z. Li
4
S. Jin
1
  1. Department of Respiratory Medicine, Fourth Affiliated Hospital, Harbin Medical University, 37# Yiyuan Street, Harbin 150001, Heilongjiang, China
  2. Department of pulmonary diseases, Heilongjiang Academy of Traditional Chinese Medicine, 33# Xidazhi Street, Harbin 150036, Heilongjiang, China
  3. Department of Intensive Care Unit, Fourth Affiliated Hospital, Harbin Medical University, 37# Yiyuan Street, Harbin 150001, Heilongjiang, China
  4. University of Tokyo, 3-8-1# Bunkyo ku, Tokyo 1130033, Tokyo, Japan
12 Topical erythritol combined with L-ascorbyl-2-phosphate inhibits staphylococcal growth and alleviates staphylococcal overgrowth in skin lesions of canine superficial pyoderma
T. Tochio , K. Kawano , K. Iyori , R. Makida , Y. Kadota , T. Fujii , H. Ishikawa , T. Yasutake , A. Watanabe , K. Funasaka , Y. Hirooka , K. Nishifuji
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 647-655 | DOI: 10.24425/pjvs.2023.148284
Słowa kluczowe: dog erythritol L-ascorbyl-2-phosphate pyoderma skin microbiota staphylococci
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Abstrakt

Erythritol (ERT) and L-ascorbyl-2-phosphate (APS) are bacteriostatic, but their effects on staphylococcal skin infections remain unknown. We aimed to determine whether ERT combined with APS inhibits the growth of staphylococci that are commonly isolated from pyoderma skin lesions in dogs. We investigated the individual and combined effects of ERT and APS on the growth of Staphylococcus pseudintermedius, S. schleiferi, and S. aureus using turbidity assays in vitro. Skin lesions from 10 dogs with superficial pyoderma were topically treated with 5% ERT and 0.1% APS for 28 days, and swabbed skin samples were then analyzed using 16S rRNA amplicon sequencing and quantitative real-time PCR (qPCR). Results showed that ERT inhibited S. pseudintermedius growth regardless of harboring the mecA gene, and APS increased the inhibitory effects of ERT against S. pseudintermedius, S. schleiferi, and S. aureus in vitro. Moreover, combined ERT and APS decreased the prevalence of staphylococci on canine skin lesions at the genus level. The combination slightly increased the α-diversity but did not affect the β-diversity of the microbiota. The qPCR results revealed that the combination significantly decreased S. pseudintermedius and S. schleiferi in skin lesions. Topical administration of EPS combined with APS can prevent staphylococcal colonization on the surface of mammalian skin. The results of this study may provide an alternative to systemic antibiotics for treating superficial pyoderma on mammalian skin surfaces.
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Bibliografia

1. Akiyama H, Oono T, Huh WK, Yamasaki O, Katsuyama M, Shigeyuki O, Ichikawa H, Iwatsuki K (2002) Actions of farnesol and xyli-tol against Staphylococcus aureus. Chemotherapy 48: 122-128.
2. Bradley CW, Morris DO, Rankin SC, Cain CL, Misic AM, Houser T, Mauldin EA, Grice EA (2016) Longitudinal evaluation of the skin microbiome and association with microenvironment and treatment in canine atopic dermatitis. J Invest Dermatol 136: 1182-1190.
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6. González-Domínguez MS, Carvajal HD, Calle-Echeverri DA, Chinchilla-Cárdenas D. (2020) Molecular detection and characterization of the mecA and nuc genes From Staphylococcus species (S. aureus, S. pseudintermedius, and S. schleiferi) isolated from dogs suffering superficial pyoderma and their antimicrobial resistance profiles. Front Vet Sci 7: 376.
7. Hashino E, Kuboniwa M, Alghamdi SA, Yamaguchi M, Yamamoto R, Cho H, Amano A (2013) Erythritol alters microstructure and metabolomic profiles of biofilm composed of Streptococcus gordonii and Porphyromonas gingivalis. Mol Oral Microbiol 28: 435-451.
8. Ikeno H, Apel M, Zouboulis C, Luger TA, Böhm M (2015) L-ascorbyl-2-phosphate attenuates NF-κB signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion. Arch Dermatol Res 307: 595-605.
9. Iwasaki T, Nagata M, Ohmuro T, Ando J (2008) Validation of a novel guideline for clinical trial of antimicrobials in canine bacterial py-oderma. Jpn J Vet Dermatol 14: 71-75.
10. Japanese Ministry of Health, Labour and Welfare Good Clinical Practice. Ministry of Health, Labour and Welfare Ministerial Ordinance, No. 28 dated March 27, 1997, and No. 24 dated February 29, 2008. Tokyo, Japan.(https://www.pmda.go.jp/files/000152996.pdf)
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12. Kawakami T, Shibata S, Murayama N, Nagata M, Nishifuji K, Iwasaki T, Fukata T (2010) Antimicrobial susceptibility and methicillin resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in Ja-pan. J Vet Med Sci 72: 1615-1619.
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Autorzy i Afiliacje

T. Tochio
1 2
K. Kawano
2 3
K. Iyori
4
R. Makida
1
Y. Kadota
1
T. Fujii
1
H. Ishikawa
5
T. Yasutake
5
A. Watanabe
2
K. Funasaka
2
Y. Hirooka
2
K. Nishifuji
6
  1. B Food Science Co., Ltd., 24-12, Kitahama-machi, Chita, Aichi 478-0046, Japan
  2. Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Aichi 470-1192, Japan
  3. Tokyo Animal Allergy Center, 4-23-15, Kurihara, Adachi-ku, Tokyo 123-0842, Japan
  4. Vet Derm Tokyo, Dermatological and Laboratory Service for Animals, 910 Shoubusawa, Fujisawa, Kanagawa 252-0823, Japan
  5. Healthcare Systems Co., Ltd., Nagoya Aichi, 466-0058, Japan
  6. Division of Animal Life Science, Institute of Agriculture, Graduate School, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
13 The effects of proanthocyanidin on testicular toxicity in rats exposed to a glyphosate-based herbicide
F. Avdatek , M. Kirikkulak , D. Yeni
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 657-666 | DOI: 10.24425/pjvs.2023.148285
Słowa kluczowe: glyphosate proanthocyanidin rat spermatozoa testicular toxicity
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Abstrakt

The purpose of this study is to determine the effects of proanthocyanidin (PA) on spermatological parameters and testicular toxicity in male rats exposed to glyphosate (GLP). In our study, four groups were formed out of 24 male rats, each group would include 6 rats. The rats in the PA group were given a dose of 400 mg/kg/day dissolved in DMSO via gastric gavage. The rats in the GLP+PA groups were first given GLP at the LD50/10 dose of 787.85 mg/kg/day, followed by administering PA at a dose of 400 mg/kg/day dissolved in DMSO via gastric gavage. The rats in the GLP group were given GLP at the LD50/10 dose of 787.85 mg/kg/day dissolved in DMSO via gastric gavage. It was determined that in terms of motility, in comparison to the control group, the decreases in the GLP group and the increases in the PA and GLP+PA groups were statistically significant (p<0.001). The administration of GLP increased DNA damage compared to the control group, but the GLP+PA and PA applications reduced DNA damage (p<0.001). The analysis of testosterone levels indicated a statistically significant reduction in the GLP group compared to the other groups. Consequently, it was determined that PA effectively prevented the decreases in the spermatological parameters lowered as a result of GLP exposure and the oxidative stress and toxicity in testicular tissue.
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Bibliografia

1. Abarikwu SO, Akiri OF, Durojaiye MA, Adenike A (2015) Combined effects of repeated administration of Bretmont Wipeout (glypho-sate) and Ultrazin (atrazine) on testosterone, oxidative stress and sperm quality of Wistar rats. Toxicol Mech Methods 25: 70-80.
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6. Avdatek F, Birdane YO, Türkmen R, Demirel HH (2018) Ameliorative effect of resveratrol on testicular oxidative stress, spermatological parameters and DNA damage in glyphosate-based herbicide-exposed rats. Andrologia 50: e13036.
7. Bashir N, Shagirtha K, Manoharan V, Miltonprabu S (2019) The molecular and biochemical insight view of grape seed proanthocya-nidins in ameliorating cadmium-induced testes-toxicity in rat model: implication of PI3K/Akt/Nrf-2 signaling. Biosci Rep 39: BSR20180515.
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Autorzy i Afiliacje

F. Avdatek
1
M. Kirikkulak
1
D. Yeni
1
  1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Afyon Kocatepe, 03030, Afyonkarahisar, Turkey
14 The effect of benign prostatic hyperplasia on total antioxidant capacity and protein peroxidation in canine prostatic fluid and spermatozoa
A. Domosławska , S. Zduńczyk , A. Bielecka , M. Kankofer
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 667-673 | DOI: 10.24425/pjvs.2023.148286
Słowa kluczowe: dog BPH prostatic fluid total antioxidant capacity protein peroxidation
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Abstrakt

The aim of this study was to evaluate the antioxidative/oxidative status of spermatozoa and prostatic fluid in dogs with benign prostatic hyperplasia (BPH) by the determination of total antioxidant capacity and protein peroxidation markers. Study was conducted on 40 intact dogs of various breeds. The dogs were assigned to two groups: BPH group (n=20) and non-affected group (n=20). The second and third fractions of the ejaculate were collected separately by digital manipulation. Total antioxidant capacity (TAC) and the concentrations of SH-groups in sperm and prostatic fluid were determined spectrophotometrically, the concentrations of bityrosine and formylkynurenine were determined using spectrofluorimetric methods. The mean values of TAC in spermatozoa and prostatic fluid were significantly lower (p<0.05), whereas the mean contents of biotyrosine and formylkinurenine were significantly higher (p<0.05) in BPH dogs compared to control dogs. There was no statistically significant difference in the content of SH group between dogs with BPH and control dogs (p>0.05). In conclusion, the results indicate that BPH in dogs is associated with reduced total antioxidant capacity and increased protein oxidation in the prostatic fluid and spermatozoa, and suggest the importance of oxidative stress in the pathogenesis of this condition. The potential role of antioxidants in the prevention and therapy of canine BPH requires further studies.
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Bibliografia

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Autorzy i Afiliacje

A. Domosławska
1
S. Zduńczyk
1
A. Bielecka
2
M. Kankofer
2
  1. Department of Animal Reproduction with Clinic, University of Warmia and Mazury, 10-719 Olsztyn, Oczapowskiego 14, Poland
  2. Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Akademicka 12, Lublin, Poland
15 Effect of organic zinc supplementation on hematological, mineral, and metabolic profile in dairy cows in early lactation
S. Dresler , J. Illek , K. Cebulska , M. Šoch
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 675-686 | DOI: 10.24425/pjvs.2023.148287
Słowa kluczowe: trace elements zinc hematocrit biochemical parameters
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Abstrakt

The aim of the current trial was to evaluate the effect of organically chelated zinc – methionin (Zn-Met) supplementation (30 mg Zn /kg DM TMR) on hematological, biochemical, and mineral profile of dairy cows in early lactation (1 - 90 d p.p.). Twenty dairy cows were randomly allocated to one of two dietary treatments in a randomized design. Animals in group C were treated as control (no zinc supplementation); whereas animals in group S were supplemented with organic Zn.
Zn-Met supplementation had a significant effect on hematological parameters. White blood cell (WBC) counts 60 days p.p. and red blood cell (RBC) count, hemoglobin concentration (HGB), hematocrit level (HCT) and platelet count (PLT) on calving day, 30th- and 60th- day p.p were significantly higher in cows fed Zn than in the control group. In calves from supplemented mothers, there was a significant increase in RBC (p≤0.001), HCT (p≤0.01) and MCV (p≤0.05).
There was no difference in other parameters among the groups, except of the highly significant difference in Zn concentration in blood serum of the S-group during the entire experimental time. The results obtained confirm the beneficial effect on serum zinc level and hematological parameters with no negative effects of 30mg Zn/kg TMR addition on mineral and biochemical parameters.
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Autorzy i Afiliacje

S. Dresler
1
J. Illek
2
K. Cebulska
3
M. Šoch
1
  1. Department of Animal Husbandry Sciences, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Studentská 1668, 370 05 České Budějovice, Czech Republic
  2. Faculty of Veterinary Medicine, Large Animal Clinical Laboratory, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic
  3. Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland
16 Pelvic dimensions and occurrence of dystocia in Black-and-White and Holstein-Friesian heifers
Z. Nogalski , W. Barański
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 687-693 | DOI: 10.24425/pjvs.2023.148288
Słowa kluczowe: dairy heifers dystocia intrapelvic area pelvimeter rump angle
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Abstrakt

The Black-and-White (BW) breed, which until recently had dominated in Europe, was replaced by the Holstein-Friesian (HF) breed. As a result, the incidence of dystocia has increased. Dystocia occurs most frequently in heifers, and it is associated with high calf weight and/or too narrow pelvic openings in heifers. The aim of this study was to evaluate retrospectively the effects of pelvic dimensions and rump angle on calving ease in two cattle breeds. The research was carried out in four barns where BW and HF cattle were used. The course of parturition was evaluated in 317 heifers (BW, n=169; HF, n=148) based on direct observations. Calves were weighed, external and internal pelvic measurements were performed (using the Rice pelvimeter), and rump angle was determined in heifers. Based on the course of parturition, heifers of both breeds were divided into easy calving (EC) and difficult calving (DC) groups. The frequency of DC was 24.3% in HF heifers and 13.1% in BW heifers. In comparison with DC heifers, EC heifers had a larger pelvic area, in particular the internal dimensions of the bony pelvis, and a higher rump angle. In comparison with BW heifers, HF heifers had a smaller rump angle, a narrower pelvis and a lower ratio of pelvic area to calf weight. High dystocia rates in HF heifers could result from a relatively large fetus size and a less preferable pelvic size and rump angle. High variation in the internal pelvic dimensions in HF heifers indicates that the incidence of dystocia can be reduced through selection for a larger pelvic size and the optimal rump angle.
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Bibliografia

1. Bila L, Fourie PJ, Tyasi TL (2022) Using pelvic areas and linear body measurements in the selection for reduced dystocia rates in Sussex heifers. Indian J Anim Res 56: 928-932.
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Autorzy i Afiliacje

Z. Nogalski
1
W. Barański
2
  1. Department of Animal Nutrition, Feed Science and Cattle Breeding, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719 Olsztyn, Poland
  2. Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719 Olsztyn, Poland
17 Association between sperm morphology and sperm count of boar semen
S. Kondracki , K. Górski , M. Iwanina , W. Kordan , M. Lecewicz
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 695-704 | DOI: 10.24425/pjvs.2023.148289
Słowa kluczowe: boar sperm count sperm dimension sperm morphology
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Abstrakt

The number of spermatozoa in the ejaculate is important for its quality and that of the sperm contained in it. The number of ejaculated spermatozoa is also associated with sperm dimensions. The aim of this study was to assess the morphological structure of sperm and the frequency of morphological abnormalities in sperm on the ejaculation performance of boars, measured as the total number sperm per ejaculate. The study was conducted using 648 ejaculates collected from 31 Large White boars and 30 Landrace boars. All ejaculates were analysed for basic physical characteristics and the frequency of sperm with morphological abnormalities. In addition, morphometric measurements of the sperm were made and used to calculate their shape indexes. As a result of our study it was noted that sperm from ejaculates with the most spermatozoa have shorter heads with a smaller area than sperm from ejaculates with a small or intermediate number of spermatozoa. Landrace boars produce semen of better quality, with a smaller percentage of sperm with major abnormalities, and the differences between the breeds increase with the number of spermatozoa in the ejaculate. The sperm from Landrace boars have larger heads and longer flagella than the sperm from Large White boars. The differences in sperm dimensions between breeds decrease as the total number of spermatozoa in the ejaculate increases. The number of spermatozoa in the ejaculate was shown to influence the dimensions of the sperm. The effect of the number of ejaculated sperm on ejaculate characteristics and sperm morphology depends on the breed of the male.
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Bibliografia

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Autorzy i Afiliacje

S. Kondracki
1
K. Górski
1
M. Iwanina
1
W. Kordan
2
M. Lecewicz
2
  1. University of Siedlce, Faculty of Agricultural Sciences, Prusa 14, 08-110 Siedlce, Poland
  2. University of Warmia and Mazury in Olsztyn, Department of Animal Biochemistry and Biotechnology, Oczapowskiego 13, 10-719 Olsztyn, Poland
18 Clinical evaluation of antiviral combination treatment in cats with feline herpesvirus-1 infection
Y. Ozkanlar , N. Ulas , I. Sozdutmaz , S. Ozkanlar
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 705-713 | DOI: 10.24425/pjvs.2023.148290
Słowa kluczowe: acyclovir antiviral treatment famciclovir feline herpesvirus L-lysine
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Abstrakt

Feline herpesvirus-1 (FHV-1) can cause lifelong problems such as rhinotracheitis and ocular disease due to latency and reactivation in affected cats. The particular effects of antiviral drugs have been separately investigated in previous studies for decades and little is known about the combination treatment in active FHV-1 infection. Therefore, we aimed to evaluate the effects of antiviral combination on clinical effectiveness in cats with naturally occurring FHV-1 infection. 28 cats suffering from clinical signs of sneezing, nasal congestion, conjunctivitis, and eye/nose discharge were involved in this study following FHV-1 DNA detection by PCR assay in oculo-oropharyngeal samples. The treatment protocol was as follows: oral famciclovir and L-lysine, ophthalmic acyclovir, and subcutaneous amoxicillin plus clavulanic acid. The symptoms improved each day and total recovery success rate was 80% reduction in clinical scores at the end of the treatment on day 10 (p<0.001). Additionally, PCR was found to be negative for FHV-1 DNA in 82.1% of the samples after the treatment. There were mild decreases in neutrophil and monocyte counts (p>0.05). The arginine to lysine ratio decreased in favour of lysine (p<0.01). As a result, the antiviral combination treatment with famciclovir, L-lysine and ophthalmic acyclovir, and antibacterial drug appears to be clinically effective for the treatment of naturally occurring active FHV-1 infection in cats. In addition, any adverse clinical effect has not been determined associated with the antiviral combination during the study.
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Bibliografia

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Autorzy i Afiliacje

Y. Ozkanlar
1
N. Ulas
2
I. Sozdutmaz
3
S. Ozkanlar
4
  1. Department of Internal Medicine, Faculty of Veterinary, Ondokuz Mayis University, 55139 Atakum/Samsun, Turkey
  2. Department of Internal Medicine, Faculty of Veterinary, Ataturk University, 25240 Yakutiye/Erzurum, Turkey
  3. Department of Virology, Faculty of Veterinary, Erciyes University, 38280 Talas/Kayseri, Turkey
  4. Department of Biochemistry, Faculty of Veterinary, Ataturk University, 25240 Yakutiye/Erzurum, Turkey
19 The influence of lactic acid bacteria on the viability of the reference strain of Listeria monocytogenes 123 serotype I in plant foods
A. Yeleussizova , P. Sobiech , N. Kaumenov , A. Batyrbekov , J. Błażejak-Grabowska , A. Isabaev , A. Platt-Samoraj
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 715-721 | DOI: 10.24425/pjvs.2023.148291
Słowa kluczowe: Listeria monocytogenes lactic acid bacteria carrot juice compound feed
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Abstrakt

Listeria (L.) monocytogenes is the causative agent of human listeriosis, the frequent sourceof which is food of animal origin. The aim of this study was to determine the influence of lactic acid bacteria (LAB) on the viability of Listeria in carrot juice and compound feed inoculated with L. monocytogenes. The effect of homogenous cultures of Streptococcus (Str.) lactis distaticus, Str. thermophilus and Lactobacillus (Lac.) lactis subsp. Cremoris and the combination of Str. thermophilus with Lac. bulgaricus in the carrot juice and compound feed samples on viability of inoculated L. monocytogenes were examined. There were no statistically significant differences in the results between the experimental groups. Regardless of used LAB, the results showed that the mean pH values in the carrot juice samples decreased from an initial pH of 6.7 to a mean value of 3.7 on 15 experimental day. The Listeria concentration in carrot juice samples decreased from average of 4.94 on day 5 to 3.24 log CFU/mL on day 10, and on day 15 achieved <0.01 log CFU/mL. In the compound feed trials, the pH decreased average from initial 6.5 to 3.7 on day 15. The concentration of Listeria decreased, similarly to the carrot juice samples, from average 5.0 on day 5 to 4.68 on day 10, and on day 15 achieved <0.01 log CFU/mL. In control samples, the number of Listeria increased throughout the study period and amounted to 9.2-9.84 log CFU/mL/g in all the samples. The activity of LAB has been shown to be antagonistic to L. monocytogenes. The results of the study did not show any clear differences between the used LAB strains in limiting the L. monocytogenes concentration. Based on the obtained results it can be conducted that the addition of LAB to animal food increases its microbiological safety.
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Bibliografia

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Autorzy i Afiliacje

A. Yeleussizova
1
P. Sobiech
2
N. Kaumenov
1
A. Batyrbekov
1
J. Błażejak-Grabowska
4
A. Isabaev
1
A. Platt-Samoraj
3
  1. Department of Veterinary Sanitation, A. Baitursynov Kostanay Regional University, Baitursynov street 47, 110000 Kostanay, Kazakhstan
  2. Department of Internal Diseases with Clinic, Faculty of Veterinary Medicine, University of Warmia-Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland
  3. Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia-Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
  4. Department of Commodity Science and Animal Improvement, Faculty of Animal Bioengineering, University of Warmia-Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland
20 Ultrasonographic image of fatty infiltration of the liver correlates with selected biochemical parameters and back fat thickness of periparturient Holstein-Friesian cows
D. Grzybowska , P. Sobiech , D. Tobolski
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 723-732 | DOI: 10.24425/pjvs.2023.148292
Słowa kluczowe: blood biochemistry dairy cattle fatty liver disease ketosis liver ultrasound subcutaneous fat transition period
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Abstrakt

During the transition period, the cow’s body activates adaptive mechanisms aimed at adjusting to the changing demand for energy and nutrients, which are necessary for the growing fetus and the subsequent start of milk production. This time is also associated with an increased risk of metabolic diseases and reproductive disorders.
Our study aimed to identify prepartum and postpartum biochemical markers and weight loss patterns that could differentiate cows that would exhibit ultrasonographic signs of liver fatty infiltration during the latter half of the transition period.
The study was performed in a single herd of Holstein-Friesian cows and the animals were divided into two groups: CON (n=13) – cows without ultrasonographic signs of fatty liver, and FL (n=16) – cows with ultrasonographic signs of fatty liver. Backfat thickness and specific biochemical parameters were measured weekly from one week before parturition to 9 weeks postpartum.
Our study highlights the importance of using a combination of monitoring methods to assess the metabolic status of transition dairy cattle. The results showed that ultrasound measurements of backfat thickness, blood NEFA levels, glucose concentration, and AST activity were all different (p<0.05) between the control and FL groups, indicating the usefulness of these parameters in monitoring the health status of transition cows. Additionally, the results suggest that high prepartum glucose levels (4.99 mmol/l) could serve as a potential marker for future FL, while the elevated NEFA levels (0.51 mmol/l) and decreased AST activity (80.56 u/l) in FL animals indicate their potential as indicators of lipid mobilization and liver structural damage, respectively.
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Bibliografia

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Autorzy i Afiliacje

D. Grzybowska
1
P. Sobiech
1
D. Tobolski
1
  1. Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland
21 Vaginal aerobic bacteria of healthy bitches and those with fertility problems
D. Jagódka , E. Kaczorek-Łukowska , R. Graczyk , P. Socha
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 733-739 | DOI: 10.24425/pjvs.2023.148293
Słowa kluczowe: aerobic bacteria bitch fertility problems vagina
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Abstrakt

The most common problems in veterinary practice in bitches are bacterial infections of the reproductive tract associated with fertility problems. Research to determine the correlation between the health status of female dogs and bacterial flora of the genital tract has been ongoing for years, but the results obtained by different authors are often contradictory, and do not always concern breeding bitches. Our study identified the most common aerobic bacteria in the genital tract of numerous breeding bitches population. A total of 275 breeding dogs in anestrous phase of the estrous cycle were included in this study. 198 were qualified to the first group with no genital tract infections and no reproductive disorders. 68 bitches were qualified to the second group with complications such as: infertility, abortion, foetus resorptions and newborn mortality. The type of bacterial isolates was almost the same in the healthy bitches and the group with fertility problems. The most common bacteria obtained from the vaginal tract of the tested dogs were Streptococcus spp., Staphylococcus spp., Mycoplasma canis and Escherichia coli. There were no significant differences in bacterial prevalence in the group with reproductive problems versus healthy dogs; however, we found a statistically significant difference between both groups when the numbers of bacterial strains were compared. The number of one-strain bitches was statistically higher in the problematic group than in the non-problematic one. Bacterial culturing of vaginal swab specimens from breeding bitches without clinical signs of genital disease is of little value. Furthermore, it should always be preceded by an examination (clinical, cytological or vaginoscopy etc.). The request or requirement to perform vaginal cultures that is made by some breeders, while common, is not diagnostic for any pathologic condition and the results of these cultures should never be used to determine if antibiotic therapy is indicated.
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Bibliografia

1. Bjurström L (1993) Aerobic bacteria occurring in the vagina of bitches with reproductive disorders. Acta Vet Scand 34: 29-34.
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5. Delucchi L, Fraga M, Perelmuter K, Cidade E, Zunino P (2008) Vaginal lactic acid bacteria in healthy and ill bitches and evaluation of in vitro probiotic activity of selected isolates. Can Vet J 49: 991-994.
6. Dzięcioł M, Niżański W, Stańczyk E, Kozdrowski R, Najder-Kozdrowska L, Twardoń J (2013) The influence of antibiotic treatment of bitches in oestrus on their attractiveness to males during mating. Pol J Vet Sci 16: 509-516.
7. Golińska E, Sowińska N, Tomusiak-Plebanek A, Szydło M, Witka N, Lenarczyk J, Strus M (2021) The vaginal microflora changes in various stages of the estrous cycle of healthy female dogs and the ones with genital tract infections. BMC Vet Res 17: 8
8. Groppetti D, Pecile A, Barbero C, Martino PA (2012) Vaginal bacterial flora and cytology in proestrous bitches: role on fertility. Theri-ogenology 77: 1549-1556.
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Autorzy i Afiliacje

D. Jagódka
1
E. Kaczorek-Łukowska
2
R. Graczyk
3
P. Socha
4
  1. AURA Veterinary Clinic, Dębowa 31, 86-065 Lochowo, Poland
  2. Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719, Olsztyn, Poland
  3. Department of Biology and Animal Environment, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland
  4. Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland

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